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Vancomycin hydrochloride CAS:1404-93-9
Vancomycin hydrochloride ke moriana o thibelang likokoana-hloko o sebelisetsoang ho phekola tšoaetso e matla e bakoang ke mefuta e itseng ea libaktheria. Ke oa sehlopha sa lithethefatsi tsa glycopeptide 'me o sebetsa ka ho thibela tlhahiso ea peptidoglycan, karolo ea bohlokoa ea marako a lisele tsa baktheria. Hangata o sebelisoa ho phekola tšoaetso e kang MRSA, letšollo le amanang le Clostridium difficile, le endocarditis ea baktheria..
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HEPPS CAS:16052-06-5 Theko ea moetsi
HEPPS, e emelang N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, ke sesebelisoa sa ho thibela zwitterionic se sebelisoang haholo lipatlisisong tsa baeloji le tsa biochemical. E tsebahala ka bokhoni ba eona bo botle ba ho thibela le botsitso holim'a pH e pharaletseng, haholo-holo pakeng tsa pH 6.8-8.2. HEPPS hangata e sebelisoa mecheng ea temo ea sele, tlhoekisong ea protheine, liketsong tsa enzymatic, le lits'ebetsong tse ling tse hlokang tikoloho ea pH e laoloang hantle. E qhibiliha ka metsing 'me hangata e sebelisoa maemong a pakeng tsa 10 mM le 100 mM. HEPPS e nkoa e se chefo ho lisele 'me e na le tšitiso e tlase ea morao, e leng se etsang hore e be khetho e tsebahalang bakeng sa liprothokholo tse ngata tsa liteko.
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2,3,5-Triphenyl-2H-tetrazolium chloride CAS:298-96-4
2,3,5-Triphenyl-2H-tetrazolium chloride, e tsejoang hape e le TTC, ke motsoako o sebelisoang haholo e le sesupo sa redox lits'ebetsong tsa baeloji le tsa lik'hemik'hale. E hlaha e le phofo e kristale e mosehla-tšoeu.
TTC e sebelisoa khafetsa litekong tse fapaneng tsa baeloji le litekong ho fumana hore na sele e phela hantle hakae le ts'ebetso ea metabolism. Ha e eketsoa liseleng tse phelang, e fokotsoa ka enzymatic ho etsa sehlahisoa sa formazan e khubelu. Phokotso ena e itšetlehile ka lienzyme tse sebetsang tsa dehydrogenase tse teng liseleng tse sebetsang. Ka lebaka leo, bongata ba formazan e khubelu e hlahisoang bo lekana le palo ea lisele tse sebetsang ka metabolism.
Bokhoni ba TTC ba ho lekola ho phela ha sele bo etsa hore e be molemo libakeng tsa lipatlisiso tse kang liteko tsa chefo ea lisele, liteko tsa ho ata ha sele, le tlhahlobo ea katleho ea lithethefatsi. Ho phaella moo, e sebelisoa ho microbiology bakeng sa ho lekola kholo ea baktheria, hammoho le lithutong tse kenyeletsang setso sa lisele tsa limela le tlhahlobo ea ho phela ha lisele.
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Letswai la Gly-Pro p-nitroanilide p-toluenesulfonate CAS: 65096-46-0
Letswai la Gly-Pro p-nitroanilide p-toluenesulfonate ke motsoako oa lik'hemik'hale o sebelisoang litekong tsa enzymatic ho lekanya ts'ebetso ea li-peptidase tse ikhethileng tsa proline. Le entsoe ka dipeptide Gly-Pro e hokahaneng le sehlopha sa p-nitroanilide, se ka lokolloang ke ts'ebetso ea li-peptidase tse ikhethileng tsa proline.
Litekong tsa enzymatic, motsoako ona o eketsoa sampole e nang le enzyme e khahlisang. Enzyme e arola tlamo pakeng tsa glycine (Gly) le proline (Pro), e lokolla sehlopha sa p-nitroanilide. P-nitroanilide e lokollotsoeng e ka lekanyetsoa ka spectrophotometric, e leng se lumellang ho fumana hore na enzyme e sebetsa joang.
Mofuta oa letsoai la p-toluenesulfonate oa Gly-Pro p-nitroanilide o fana ka botsitso le ho qhibiliha ha litharollo tsa metsi, o nolofatsa tšebeliso ea oona litekong tsa enzymatic..
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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8
5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ke motsoako o sebelisoang lithutong tse fapaneng tsa biochemical, haholo-holo bakeng sa ho lemoha le ho bona tšebetso ea enzyme. Ke substrate e ka hydrolyzed ke li-enzyme tse itseng, e leng se fellang ka ho lokolloa ha sehlahisoa se 'mala kapa se khanyang.
Motsoako ona o sebediswa hangata ditekong ho lemoha boteng le tshebetso ya dienzyme tse kang beta-galactosidase le beta-glucuronidase. Dienzyme tsena di arola dihlopha tsa acetyl le glucosaminide ho tloha substrate, e leng se lebisang ho thehweng ha chromophore e putswa kapa e tala.
Sebopeho se ikhethang sa 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide se dumella ho lemohuwa habonolo le ho lekanya tshebetso ya enzyme. Tshebediso ya yona mekgweng e fapaneng ya diteko, ho kenyeletswa histochemistry, immunohistochemistry, le diteko tse thehilweng sele, e kentse letsoho kutlwisisong e betere ya mesebetsi ya enzyme.
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AMPPD CAS: 122341-56-4 Theko ea moetsi
AMPPD, khutšoanyane bakeng sa 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, ke karoloana ea chemiluminescent e sebelisoang haholo lits'ebetsong tsa baeloji le tsa tlhahlobo ea 'mele ea ho itšireletsa mafung. E sebelisoa haholo lipatlisisong tsa baeloji ea limolek'hule, tlhahlobong ea mafu le mahlaleng a bophelo.
AMPPD e etselitsoe ka ho khetheha ho sebelisoa hammoho le li-conjugates tsa alkaline phosphatase enzyme. Ha e pepesehetse alkaline phosphatase, AMPPD e feta karabelong e potlakileng le e sebetsang ea enzyme cleavage, e lebisang ho ntšeng ha leseli.
Leseli le ntšitsoeng ho tsoa ho AMPPD le fumanoa ka ho sebelisa litsamaiso tsa litšoantšo tse bonolo kapa li-luminometer, tse lumellang ho lekanya le ho bona limolek'hule kapa li-analyte tse reretsoeng. Mokhoa ona oa ho lemoha o thehiloeng ho chemiluminescence o fana ka kutlo e phahameng, lerata le tlase la bokamorao, le mefuta e mengata e fapaneng ea matla.
Ka lebaka la botsitso ba eona le lets'oao le tšoarellang nako e telele, AMPPD e loketse hantle lits'ebetsong tse kang Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, le in situ hybridization. E nolofalletsa bafuputsi ho lemoha le ho sekaseka liprotheine, nucleic acid, li-antibodies le li-biomolecule tse ling.
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2,6-Dichloroindophenol sodium letsoai CAS: 620-45-1
3,5-Dichlorophenyldiazonium tetrafluoroborate ke motsoako oa lik'hemik'hale o sebelisoang haholo tlhahisong ea lintho tse phelang. Ke letsoai la diazonium, ho bolelang hore le na le sehlopha sa diazonium (N2+) se hoketsoeng lesale la phenyl le nkeloang sebaka ke liathomo tsa chlorine maemong a 3 le a 5.
Motsoako ona o atisa ho sebelisoa liketsong tse kenyeletsang ho kopana le metsoako e meng e nkhang hamonate. O ka feta liketsong tse kang ho nkeloa sebaka ha monko o monate ka electrophilic, moo o ka fetisetsang sehlopha sa oona sa diazonium ho motsoako o mong o nkhang hamonate oa nucleophilic ho theha kamano e ncha. Sena se thusa haholo bakeng sa ho kenyelletsa lihlopha tse sebetsang masaleng a monko o monate..
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3,3′-Diaminobenzidine CAS:91-95-2
3,3′-Diaminobenzidine (DAB) ke motsoako oa lik'hemik'hale o sebelisoang haholo mekhoeng ea ho taka immunohistochemistry (IHC) le immunocytochemistry (ICC). Ke substrate ea chromogenic e hlahisang sehlahisoa sa karabelo e sootho ha e pepesehetse li-enzyme tsa peroxidase, e leng se bontšang boteng ba antigen e reriloeng.
Hangata DAB e sebediswa ho bona diprotheine kapa dimolekhule tse ding tse kgahlehang ka hara disampole tsa baeloji, tse kang dikarolo tsa dinama kapa disele. E ka fana ka letshwao le fumanehang sebakeng se itseng le le bonahalang bakeng sa antigen e rerilweng, e leng se dumellang bafuputsi ho hlwaya le ho sekaseka kabo le bongata ba yona.
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(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid CAS:53188-07-1
Trolox ke motsoako oa maiketsetso o leng oa sehlopha sa li-antioxidants tse tsejoang e le li-analog tsa vithamine E. Ke antioxidant e matla e ka senyang li-free radical le ho sireletsa lisele le lisele ho tsoa tšenyo ea oxidative. Trolox e ithutiloe ka botlalo bakeng sa melemo ea eona ea bophelo bo botle, ho kenyeletsoa bokhoni ba eona ba ho fokotsa ho ruruha, ho thibela tšenyo ea lisele le ho tšehetsa bophelo bo botle ba pelo le methapo ea mali. E sebelisoa hangata e le motsoako oa litšupiso lipatlisisong tsa antioxidant le e le tlatsetso lijong tse fapaneng le lihlahisoa tsa litlolo.
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride CAS:25952-53-8
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, e tsejoang hape e le EDC hydrochloride kapa EDAC, ke motsoako oa lik'hemik'hale o sebelisoang haholo ho biochemistry le baeloji ea limolek'hule e le moemeli oa ho kopanya bakeng sa ho kenya tšebetsong lihlopha tsa carboxyl. Ke phofo e tšoeu ea kristale e qhibilihang haholo metsing.
EDC hydrochloride e sebediswa haholoholo ho kopanyeng dipeptide le diprotheine ka ho thusa ho thehwa ha dibonto tsa amide pakeng tsa dihlopha tsa carboxyl le amino. E sebetsa ka ho kenya tshebetsong dihlopha tsa carboxyl, tse arabelang le dihlopha tsa amino boteng ba moemedi wa ho kopanya jwalo ka N-hydroxysuccinimide (NHS). Karabelo ena e fella ka ho thehwa ha dibonto tsa amide tse tsitsitseng.
EDC hydrochloride e boetse e sebediswa dipatlisisong tsa DNA le RNA bakeng sa ho fetola le ho hokahanya di-nucleic acid, hammoho le ho thibela dienzyme le di-biomolecule tse ding ho di-surface. Ke reagent e feto-fetohang e ka ntlafatsang bokgoni le ho hlaka ha dikarabelo tsena.
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HEPES CAS: 7365-45-9 Theko ea moetsi
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) ke sesebelisoa sa baeloji se sebelisoang haholo lipatlisisong tsa baeloji le tsa lik'hemik'hale. E tsebahala ka bokhoni ba eona ba ho boloka pH e tsitsitseng litharollong tsa metsi, e leng se etsang hore e lokele lits'ebetso tse fapaneng tse kenyeletsang lits'ebetso tsa sele le tsa enzymatic. HEPES ke motsoako oa zwitterionic, ho bolelang hore o na le litefiso tse ntle le tse mpe lihlopheng tse fapaneng tsa ts'ebetso, e leng se e lumellang ho hanela liphetoho pH tse bakoang ke ho eketsoa ha li-acid kapa metheo. E sebelisoa khafetsa moetlong oa sele, litekong tsa enzyme, lithutong tsa protheine, litekong tsa electrophoresis, le mefuteng ea meriana.
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p-Nitrophenyl -aL-Fucopyranoside CAS:10231-84-2
p-Nitrophenyl-α-L-Fucopyranoside ke motsoako oa lik'hemik'hale o leng oa sehlopha sa nitrophenyl glycosides. E sebelisoa hangata lipatlisisong tsa biochemical e le substrate ea enzyme α-L-fucosidase, e susumetsang hydrolysis ea eona. Motsoako ona ha o na 'mala ebile oa qhibiliha ka har'a li-solvents tsa tlhaho, e leng se etsang hore o be bonolo bakeng sa liteko tsa enzymatic.
