5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ke motsoako o sebelisoang lithutong tse fapaneng tsa biochemical, haholo-holo bakeng sa ho lemoha le ho bona tšebetso ea enzyme. Ke substrate e ka hydrolyzed ke li-enzyme tse itseng, e leng se fellang ka ho lokolloa ha sehlahisoa se 'mala kapa se khanyang.

Motsoako ona o sebediswa hangata ditekong ho lemoha boteng le tshebetso ya dienzyme tse kang beta-galactosidase le beta-glucuronidase. Dienzyme tsena di arola dihlopha tsa acetyl le glucosaminide ho tloha substrate, e leng se lebisang ho thehweng ha chromophore e putswa kapa e tala.

Sebopeho se ikhethang sa 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide se dumella ho lemohuwa habonolo le ho lekanya tshebetso ya enzyme. Tshebediso ya yona mekgweng e fapaneng ya diteko, ho kenyeletswa histochemistry, immunohistochemistry, le diteko tse thehilweng sele, e kentse letsoho kutlwisisong e betere ya mesebetsi ya enzyme.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ke substrate ya chromogenic e sebediswang haholo bakeng sa ho lemoha tshebetso ya beta-glucuronidase (GUS). GUS ke enzyme e fumanwang dipheding tse fapaneng, ho kenyeletswa dibaktheria, dimela le diphoofolo tse anyesang. X-Gluc e sebediswa kgafetsa ditekong tsa baqolotsi ba ditaba ba GUS le dipatlisisong tsa baeloji ya dimolek'hule.

Tšebeliso e ka sehloohong ea X-Gluc ke mekhoa ea ho taka ka histochemical, moo e ka bonang ponahalo le ts'ebetso ea enzyme ea GUS. Karolo ena e ka kenella ka har'a sele 'me e fetoha hydrolyzed ke GUS, e leng se fellang ka ho thehoa ha sehlahisoa se putsoa kapa se sa qhibiliheng. Ho taka hona ho putsoa ho lumella bafuputsi ho khetholla le ho fumana ts'ebetso ea GUS liseleng, liseleng le linthong tse phelang kaofela.

X-Gluc e ka boela ea sebelisoa litekong tsa bongata ho lekanya ts'ebetso ea enzyme ea GUS. Matla a 'mala o moputsoa kapa bongata ba sehlahisoa se entsoeng bo ka amahanngoa le boemo ba tlhahiso ea GUS kapa ts'ebetso ea eona ea enzyme.

Ho phaella moo, X-Gluc e sebelisitsoe dipatlisisong tsa lefutso la dimela ho ithuta tlhahiso ya lefutso, mosebetsi wa promoter, le phetoho ya dimela. E boetse e sebedisitswe ditsamaisong tsa baktheria bakeng sa ho etsa cloning le ho lemoha diprotheine tsa GUS fusion.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C16H18BrClN2O6
Teko	99%
Ponahalo	Phofo e tšoeu
Nomoro ea CAS	4264-82-8
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.