AMPPD CAS: 122341-56-4 Theko ea moetsi

AMPPD, khutšoanyane bakeng sa 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, ke karoloana ea chemiluminescent e sebelisoang haholo lits'ebetsong tsa baeloji le tsa tlhahlobo ea 'mele ea ho itšireletsa mafung. E sebelisoa haholo lipatlisisong tsa baeloji ea limolek'hule, tlhahlobong ea mafu le mahlaleng a bophelo.

AMPPD e etselitsoe ka ho khetheha ho sebelisoa hammoho le li-conjugates tsa alkaline phosphatase enzyme. Ha e pepesehetse alkaline phosphatase, AMPPD e feta karabelong e potlakileng le e sebetsang ea enzyme cleavage, e lebisang ho ntšeng ha leseli.

Leseli le ntšitsoeng ho tsoa ho AMPPD le fumanoa ka ho sebelisa litsamaiso tsa litšoantšo tse bonolo kapa li-luminometer, tse lumellang ho lekanya le ho bona limolek'hule kapa li-analyte tse reretsoeng. Mokhoa ona oa ho lemoha o thehiloeng ho chemiluminescence o fana ka kutlo e phahameng, lerata le tlase la bokamorao, le mefuta e mengata e fapaneng ea matla.

Ka lebaka la botsitso ba eona le lets'oao le tšoarellang nako e telele, AMPPD e loketse hantle lits'ebetsong tse kang Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, le in situ hybridization. E nolofalletsa bafuputsi ho lemoha le ho sekaseka liprotheine, nucleic acid, li-antibodies le li-biomolecule tse ling.

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