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TAPSO CAS: 68399-81-5 Theko ea moetsi
TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) ke buffer ea zwitterionic e sebelisoang haholo lipatlisisong tsa baeloji le tsa biochemical. Ke sesebelisoa se sebetsang hantle sa buffer se nang le pKa e haufi le pH ea 'mele, e leng se etsang hore e lokele ho boloka pH e tsitsitseng litekong tsa baeloji. TAPSO hangata e sebelisoa ho hloekiseng protheine, litekong tsa enzyme, setso sa sele le lits'ebetsong tsa electrophoresis. Ho qhibiliha ha eona ka metsi a mangata le tšitiso e tlase lits'ebetsong tsa baeloji ho e etsa khetho e tsebahalang sechabeng sa mahlale. TAPSO e tsebahala ka phello ea eona e fokolang mosebetsing oa enzyme 'me hangata e sebelisoa e le mokhoa o mong oa ho sebelisa li-agent tse ling tsa buffer joalo ka Tris kapa phosphate buffers.
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6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, e tsejoang hape e le N-acetylglucosamine thiolactone, ke motsoako oa lik'hemik'hale o nang le sebopeho se ikhethileng se nang le lesale la pyridine, sehlopha sa acetylamino, le karolo ea thiolactone.
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Sodium Glycocholate CAS: 863-57-0
Sodium glycocholate ke motsoako oa letsoai la bile o thusang ho sileng le ho monya mafura a lijo. E entsoe ka glycine le cholic acid 'me e phetha karolo ea bohlokoa ho aroleng mafura hore e be marotholi a manyenyane, e eketsa sebaka sa 'ona bakeng sa tšilo ea lijo e sebetsang hantle. Sodium glycocholate e qhibiliha haholo metsing 'me e sebelisoa mefuteng ea meriana ho ntlafatsa ho qhibiliha le ho fumaneha ha meriana e sa qhibiliheng hantle. Ho feta moo, e sebelisoa lipatlisisong le libakeng tsa laboratori ho tšehetsa kholo ea libaktheria tse itseng le mefuta ea lisele..
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TAPS Letswai la sodium CAS: 70331-82-7
Letswai la sodium la TAPS, le tsejoang hape e le N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid sodium salt, ke sesebelisoa se sebetsang ka ho tenyetseha sa ho boloka pH le taolo ya pH. E sebediswa haholo dipatlisisong tsa baeloji le tsa dikhemikhale, meriana le dihlahlobo. TAPS-Na ke motswako wa zwitterionic o thusang ho boloka pH e tsitsitseng ditharollong, o etsang hore e lokele maemo a fapaneng a diteko. Ditshebediso tsa yona di kenyeletsa setso sa sele, dipatlisiso tsa protheine, electrophoresis, tlhahiso ya dikhemikhale le diforomo tsa meriana. TAPS-Na ke mofuta o qhibilihang le o tsitsitseng wa TAPS o bonolo ho o sebetsana le ho o sebedisa ditharollong tse metsi.
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NBD chloride CAS: 10199-89-0
NBD chloride, e tsejoang hape e le 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ke dae e fluorescent e sebelisoang haholo lits'ebetsong tsa lik'hemik'hale le tsa baeloji. Ke ea sehlopha sa li-derivatives tsa nitrobenzoxadiazole (NBD), tse bontšang thepa e matla ea fluorescence.
Kloride ea NBD e khetholloa ka bokhoni ba eona ba ho theha maqhama a covalent le li-nucleophile, tse kang li-amine tsa mantlha, li-thiol, le lihlopha tsa hydroxyl. Thepa ena e e lumella ho sebelisoa e le moemeli oa ho ngola mabitso bakeng sa li-biomolecule, ho kenyeletsoa liprotheine, li-peptide, li-nucleic acid le limolek'hule tse ling tse nyane.
Ha NBD chloride e arabela le nucleophile, e ba le karabelo e nkeloang sebaka, e leng se fellang ka ho thehwa ha sehlahisoa se tsitsitseng se nang le leibole ea NBD. Sehlahisoa sena se nang le leibole se bontša tlhahiso e matla ea fluorescence, e leng se etsang hore e be molemo bakeng sa mekhoa e fapaneng ea ho lemoha le ho etsa litšoantšo.
Ho ntšoa ha khanya ea limolek'hule tse nang le leibole ea NBD ho ka tsosoa ke khanya ea ultraviolet (UV) hoo e ka bang 470 nm, 'me khanya e ntšoang hangata e bonoa hoo e ka bang 530 nm. Leqhubu lena la maqhubu a ho ntšoa ha khanya le oela sebakeng se setala sa spectrum e bonahalang, moo tšitiso ea ho lemoha ho tsoa lisampoleng tsa baeloji e leng tlase haholo.
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Protheine K CAS:39450-01-6 Theko ea moetsi
Proteinase K ke enzyme e sebetsang haholo ea serine protease e ka susumetsang hydrolysis ea litlamo tsa peptide liprotheineng. E nkiloe ho fungus Engyodontium album 'me e tsejoa ka mefuta e mengata ea eona ea ho ikhetha ha substrate. Proteinase K e ka arola litlamo tsa peptide ka katleho lehlakoreng la C-terminal la li-amino acid tse sa rateheng, tse sa rateheng, kapa tse nkhang hamonate, e leng se etsang hore e be molemo lits'ebetsong tse fapaneng tsa baeloji ea limolek'hule le biochemistry..
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N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
N,N-Dimethyl-p-phenylenediamine dihydrochloride ke motsoako oa lik'hemik'hale o sebelisoang haholo lits'ebetsong tse fapaneng tsa saense le tsa indasteri. Ke phofo ea kristale e tšoeu ho isa ho e tšoeu e nang le foromo ea limolek'hule ea C8H14N2·2HCl.
Motsoako ona o na le mefuta e mengata ea ts'ebeliso ka lebaka la thepa ea oona le karabelo ea oona. Hangata o sebelisoa e le sesebelisoa sa ho sekaseka bakeng sa ho fumana li-peroxide le e le sesebelisoa sa ho kopanya dae tlhahisong ea mebala le mebala e fapaneng.
Lefapheng la k'hemistri ea tlhahlobo, N,N-Dimethyl-p-phenylenediamine dihydrochloride e sebelisoa khafetsa e le sesupo sa colorimetric bakeng sa ho fumana mefuta ea oxidative, joalo ka hydrogen peroxide kapa peroxide lisampoleng. Motsoako o feta phetohong ea 'mala ha o arabela ka metsoako ena ea oxidizing, e leng se lumellang ho lekanya ha tsona.
Ho feta moo, motsoako ona o ka boela oa fumanoa mofuteng oa lidae tsa moriri, moo o sebetsang e le moqapi oa mebala. Bokhoni ba oona ba ho hlahisa mebala e khanyang le e tšoarellang nako e telele bo etsa hore o ratoe indastering ea litlolo.
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Letswai la phosphate disodium la Creatine CAS:922-32-7
Letswai la disodium la Creatine phosphate ke motsoako o nang le creatine, e leng ntho e tswang ho amino acid ka tlhaho, e tlamilweng dihlopheng tsa phosphate le di-ion tsa sodium. E bapala karolo ya bohlokwa ho faneng ka matla bakeng sa ho honyela ha mesifa mme hangata e sebediswa e le tlatsetso ya dijo ke baatlelete le bakganni ba mmele.
Ha e jeoa, letsoai la disodium la creatine phosphate le fetoloa creatine phosphate 'meleng, ebe le bolokoa mesifa. Nakong ea mesebetsi e matla haholo, joalo ka ho phahamisa litšepe kapa ho matha ka lebelo le phahameng, creatine phosphate e sebetsa e le mohloli o fumanehang habonolo oa litlamo tsa phosphate tse nang le matla a mangata, e leng se lumellang ho tlatsa adenosine triphosphate (ATP) ka potlako.
ATP ke molek'hule ea matla ea mantlha e sebelisoang ke mesifa, 'me maemo a eona a fokotseha ka potlako nakong ea boikoetliso bo matla. Ka ho eketsa creatine phosphate, baatlelete ba ka eketsa ho fumaneha ha ATP, e leng se lebisang ho ntlafatseng ts'ebetso ea mesifa, matla a eketsehileng le ho fokotsa mokhathala.
Ho phaella moo, letsoai la creatine phosphate disodium le ka ba le melemo e meng e ka bang teng, ho kenyeletsoa le ho eketseha ha mesifa, ho hlaphoheloa ho ntlafetseng, le ts'ebetso e ntlafetseng ea kelello. Hangata e nooa ka molomo ka mokhoa oa phofo kapa letlapa, ebang ke tlatsetso e ikemetseng kapa e le karolo ea mokhoa oa pele ho boikoetliso kapa oa tlatsetso ea kamora boikoetliso.
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2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-NHS ester ke motsoako oa lik'hemik'hale o nang le sebopeho se rarahaneng sa limolek'hule. E na le sehlopha sa sulfopropylacridinium le sehlopha se sebetsang sa ester ea carboxylate. Boteng ba karolo ea ester bo bontša hore e arabela 'me e ka sebelisoa e le moemeli oa ho ngola kapa oa ho fetola limolek'hule.
Sehlopha sa sulfopropylacridinium sa motsoako ona se fana ka maikutlo a hore se ka ba le lits'ebeliso tse ka bang teng litekong tse thehiloeng ho fluorescence, moo se ka sebelisoang e le probe ea fluorescent kapa dae bakeng sa ho lemoha le ho sekaseka limolek'hule tsa biomolecule. E ka boela ea ba le bohlokoa lithutong tsa lits'ebetso tsa sele, joalo ka ho supa calcium ka hare ho sele.
Ho kenyelletsoa ha sehlopha sa NHS ester ho bontša hore se ka arabela le li-amine tsa mantlha, tse kang tse fumanoang liprotheineng kapa li-peptide, ho theha li-amide bond tse tsitsitseng. Ho arabela hona ho etsa hore e be molemo bakeng sa lits'ebetso tsa bioconjugation, moo e ka sebelisoang ho ngola kapa ho fetola li-biomolecule ka limolek'hule tse ling tse sebetsang, tse kang li-fluorophore kapa li-tag.
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ABEI CAS: 66612-29-1 Theko ea Moetsi
N-(4-Aminobutyl)-N-ethylisoluminol, e tsejoang hape e le ABEI, ke motsoako o khanyang o atisang ho sebelisoa e le probe ea chemiluminescent lits'ebetsong tsa bioanalytical. Ke oa sehlopha sa li-derivatives tsa isoluminol 'me o sebelisoa haholo litekong tsa immunoalysis le mekhoa ea ho lemoha DNA/RNA.
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Z-GLY-PRO-PNA CAS: 65022-15-3
Z-GLY-PRO-PNA ke motsoako oa peptide nucleic acid (PNA) o nang le tatellano ea Z-Gly-Pro. Motsoako ona o sebelisoa hangata lipatlisisong le tlhahlobong ea mafu ka lebaka la bokhoni ba oona ba ho hokahana ka ho khetheha le tatellano ea nucleic acid e tlatsanang. Ho eketsoa ha sehlopha sa Z ho N-terminus ho lumella ho shebana ka katleho le ho nehelana ka lipheo tse lakatsehang tsa limolek'hule. Ka motsoako oa eona o ikhethang oa thepa ea peptide le nucleic acid, Z-GLY-PRO-PNA e fana ka bokhoni bo boholo lits'ebetsong tse fapaneng, ho kenyeletsoa kalafo ea liphatsa tsa lefutso, tlhahlobo ea limolek'hule le nts'etsopele ea lithethefatsi..
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TRIS-Acetate CAS: 6850-28-8 Theko ea moetsi
TRIS-Acetate, ke sesebelisoa se sebelisoang haholo litekong tsa baeloji le tsa biochemical. Ke motsoako oa Tris base le acetic acid, e leng se fellang ka tharollo e tsitsitseng ea pH e sebelisoang ho laola le ho boloka mefuta e lakatsehang ea pH bakeng sa lits'ebetso tse fapaneng. TRIS-Acetate e na le thuso haholo lithutong tsa DNA le RNA, kaha e fana ka tikoloho e loketseng mesebetsi ea enzyme, electrophoresis, le gel electrophoresis. E thusa ho boloka botsitso le botšepehi ba nucleic acid nakong ea mekhoa e fapaneng, joalo ka ho latellana ha DNA, polymerase chain reaction (PCR), le agarose gel electrophoresis. Ntle le lipatlisiso tsa nucleic acid, TRIS-Acetate e boetse e sebelisoa mekhoeng ea ho itšehla thajana le ho hloekisa protheine, ho ntšoa ha protheine ea membrane, le liteko tsa setso sa sele. Bokhoni ba eona ba ho thibela ho ruruha bo e etsa sesebelisoa sa bohlokoa libakeng tse ngata tsa lipatlisiso tsa mahlale, ho netefatsa maemo a matle bakeng sa liketso tsa baeloji le ho boloka botsitso ba li-enzyme le liprotheine.
