3,3′-Diaminobenzidine CAS:91-95-2

Phello ea 3,3'-Diaminobenzidine (DAB) ke ho hlahisa 'mala o sootho ha e arabela le li-enzyme tsa peroxidase. Phetoho ena ea 'mala e lumella pono ea li-antigen tse reretsoeng kapa li-biomolecule tse nang le thahasello litekong tsa ho taka tsa immunohistochemistry (IHC) le immunocytochemistry (ICC). Hangata DAB e sebelisoa lipatlisisong tsa bongaka le lits'ebetsong tsa tlhahlobo ho lemoha le ho fumana li-antigen kapa liprotheine tse itseng ka har'a lisampole tsa baeloji. Ka ho eketsa DAB e le substrate le karabelo e latelang ka li-enzyme tsa peroxidase, ho thehoa ha sootho sebakeng sa li-antigen tse reretsoeng. Tšebeliso ea ho taka ha DAB e thusa haholo ho ithuteng mafu le maemo a fapaneng. E nolofalletsa bafuputsi le litsebi tsa mafu ho bona le ho sekaseka kabo, bongata le sebaka sa li-biomolecule tse itseng liseleng kapa liseleng. Tlhahisoleseding ena e thusa ho utlwisisa mekgwa ya lefu, ho ithuta mekgwa ya sele, le ho hlwaya dipheo tse ka bang teng tsa tlhahlobo kapa kalafo. Ho feta moo, ho taka DAB ho ka sebediswa mekgweng e fapaneng ya dimolek'hule ho batlisisa dikamano tsa protheine le protheine, ho hlwaya mekgwa ya tlhahiso ya protheine, ho fumana boteng ba matshwao a itseng, le ho lekola katleho ya mekgwa ya kalafo. Ka lebaka la ho taka ha yona ho tshepahalang le ho matla, hammoho le ho tsamaellana ha yona le ditsamaiso tse fapaneng tsa ho lemoha le mekgwa ya microscopy, DAB e se e fetohile substrate ya chromogenic e sebediswang haholo ditekong tsa immunohistochemistry le immunocytochemistry.