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4-Fluoro-7-nitrobenzofurazan CAS:29270-56-2
4-Fluoro-7-nitrobenzofurazan ke motsoako oa lik'hemik'hale o nang le foromo ea limolek'hule C6H2FN3O3. Ke kristale e mosehla e tiileng e sebelisoang haholo-holo e le reagent liketsong tse fapaneng tsa lik'hemik'hale. 4-Fluoro-7-nitrobenzofurazan e tsebahala ka bokhoni ba eona ba ho arabela le li-amine tsa mantlha, e leng se etsang li-derivatives tsa fluorescent tse ka bang molemo lits'ebetsong tsa tlhahlobo tse kang ho ngola liprotheine le tlhahlobo ea amino acid. E boetse e sebelisoa lithutong tse amanang le kinetics ea enzyme le ho fumana tatellano ea li-nucleic acid.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium letsoai CAS:18656-96-7
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Letswai la cyclohexylammonium, le tsejwang hape e le X-glucuronide, ke motsoako wa dikhemikhale o sebediswang haholo ditekong tsa baeloji ya dimolek'hule le enzymology. Ke substrate ya chromogenic e atisang ho sebediswa ho lemoha mosebetsi wa enzyme beta-D-glucuronidase.
Motswako ona o na le sehlopha sa indole se kopantsoeng le karolo ea glucuronic acid ka tlamo ea glycosidic. Lintho tse nkeloang sebaka ke bromo le chloro li fana ka botsitso le ho ntlafatsa ho qhibiliha ha motsoako.
Ha e futhumatsoa ka sampole e nang le beta-D-glucuronidase, enzyme e kgaola tlamo ya glycosidic, e lokolla karolo ya indole. Karabelo ena e fella ka ho thehwa ha precipitate e putsoa kapa phetoho ya mmala, e leng se dumellang ho lemoha habonolo mosebetsi wa enzyme ka pono kapa ka spectrophotometric.
Ka lebaka la ho ikhetha ha eona bakeng sa beta-D-glucuronidase, X-glucuronide e sebelisoa haholo mekhoeng ea baeloji ea limolek'hule joalo ka liteko tsa phetoho ea baktheria le tomoso, liteko tsa liphatsa tsa lefutso tsa reporter, le ho taka ka histochemical. E fana ka mokhoa o bonolo le o bonolo oa ho lemoha boteng kapa tlhahiso ea beta-D-glucuronidase 'me hangata e sebelisoa hammoho le liteko tse ling tsa liphatsa tsa lefutso kapa tsa biochemical.
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8-Anilino-1-naphthalenesulfonic acid CAS: 82-76-8
8-Anilino-1-naphthalenesulfonic acid, e tsejoang hape e le ANS, ke dae e khanyang e sebelisoang haholo lipatlisisong tsa biochemical le biophysical. Ke ea sehlopha sa metsoako ea lintho tse phelang e tsejoang e le li-sulfonic acid tse thehiloeng ho aniline. ANS e tsebahala ka bokhoni ba eona ba ho ikamahanya le libaka tse sa rateng metsi kapa libaka tse pepesehileng tsa hydrophobic liprotheineng le limolek'hule tse ling.
ANS e phatsima ha e tlameletsoe libakeng tse sa rateng metsi, e leng se etsang hore e be probe e thusang ho ithuta liphetoho tsa sebopeho sa protheine, likamano tsa protheine le protheine, le lits'ebetso tsa ho mena/ho phutholoha ha protheine. Ho tlamahana ha ANS le libaka tse sa rateng metsi ho lebisa phetohong e putsoa ho spectrum ea emission, e ka hlahlojoang ka ho sebelisa fluorescence spectroscopy.
Hangata ANS e sebelisoa mekhoeng e fapaneng ea lipatlisiso, ho kenyeletsoa le polarization ea fluorescence, phetisetso ea matla a fluorescence resonance (FRET), le liteko tsa botsitso ba mocheso. E sebelisitsoe ho ithuta sebopeho le botsitso ba liprotheine, likamano tsa protheine le ligand, le lits'ebetso tsa ho kopanya liprotheine.
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CHLOROPHENOLRED-BETA-D-GALACTOPYRANOSIDE, SODIUM LETSOAI CAS:99792-50-4
CHLOROPHENOLRED-BETA-D-GALACTOPYRANOSIDE, SODIUM SALT ke motsoako oa lik'hemik'hale o sebelisoang haholo litekong tsa laboratori ho fumana boteng ba lienzyme tse ka senyang lactose, joalo ka beta-galactosidase. Ke phofo e se nang 'mala ho isa ho e mosehla o moputsoa e fetohang e khubelu ha lienzyme tsena li le teng, e leng se etsang hore e be sesupo se molemo bakeng sa ts'ebetso ea tsona. Sebopeho sa letsoai la sodium sa motsoako ona se ntlafatsa ho qhibiliha ha sona litharollong tsa metsi, e leng se lumellang ho lokisoa le ho sebelisoa habonolo litekong tse fapaneng tsa enzymatic.
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Tert-Butylamine CAS 75-64-9 – Boleng bo Tšepahalang bakeng sa Likopo tse Tsoetseng Pele
Tert-Butylamine (CAS 75-64-9) e hlahella ka bohloeki ba eona bo phahameng, ts'ebetso e tsitsitseng, le botsitso bo botle ba lik'hemik'hale. Amine ena ea aliphatic e feto-fetohang e ananeloa ka lebaka la ho qhibiliha ha eona ho ikhethang le ho lumellana ha eona liindastering tse fapaneng. Melemo ea bohlokoa e kenyelletsa katleho ea eona e le motheo oa tlhahiso ea lintho tse phelang, e mahareng meriana, le ho potlakisa ts'ebetso ea rabara. Tert-Butylamine e boetse e fumana bohlokoa lik'hemik'haleng tsa temo le e le thibelo ea mafome lilubricants. Ho khetha sehlahisoa sa rona ho bolela ho sebetsa le litsebi tse behang pele taolo e thata ea boleng le litharollo tsa bareki tse etselitsoeng bareki, ho netefatsa hore u fumana phepelo e tšepahalang, e nakong ka tšehetso ea tekheniki. Na u na le thahasello ea ho eketsa ts'ebetso bakeng sa projeke ea hau e latelang? Ikopanye le rona hona joale 'me re buisane ka litlhoko tsa hau!
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Neocuproine CAS 484-11-7 - Ligand e Nepahetseng bakeng sa Lipatlisiso tse Tsoetseng Pele
Neocuproine (CAS 484-11-7) e hlahella e le sesebelisoa se ikhethang sa chelating se hloekileng haholo, se tsebahalang ka khetho ea sona e ikhethang mabapi le li-ion tsa koporo (I). Ho tsitsa ha eona ka lik'hemik'hale tse matla le sebopeho sa kristale se hlalositsoeng hantle ho e etsa khetho e ratoang lilaboratoring tsa thuto le tsa indasteri. Melemo ea bohlokoa e kenyelletsa ts'ebetso ea eona e tšepahalang lithutong tsa spectroscopic, lipatlisiso tsa bioinorganic, le catalysis, hammoho le ho lumellana ha eona le lits'ebetso tse fapaneng tsa tlhahlobo le tsa maiketsetso. Bokhoni bo pakiloeng ba Neocuproine bo thusa nts'etsopele ea mekhoa ea ho lemoha e bonolo le metsoako e mecha lipatlisisong tsa lik'hemik'hale le tsa biochemical. Ho re khetha ho netefatsa hore u fumana boleng bo lekiloeng ka thata, mohloli o pepeneneng, le tšehetso ea botekgeniki ea litsebi e etselitsoeng litlhoko tsa hau tse ikhethang. Haeba u labalabela ho tseba hore na Neocuproine e ka ntlafatsa projeke ea hau joang, u se ke ua tsilatsila ho ikopanya le 'na—khatelopele ea hau e latelang ea lipatlisiso e ka qala mona. Ikopanye le 'na kajeno ho buisana ka litlhoko tsa hau kapa ho kopa khotheishene!
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Tapso Sodium CAS: 105140-25-8 Theko ea moetsi
3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,Ke 'nasa zwitterionic buffering agent ka sebopeho sa letsoai la sodium. E sebelisoa hangata litekong tsa biochemical le tsa baeloji ea limolek'hule bakeng sa ho boloka pH e tsitsitseng litharollong.It E qhibiliha haholo metsing 'me e na le pKa e haufi le pH ea fisioloji, e leng se etsang hore e lokele mefuta e mengata ea lits'ebetso tse kang tlhoekiso ea protheine, liteko tsa enzyme, setso sa sele le electrophoresis. Sebopeho sa eona se ikhethang sa lik'hemik'hale le ho lumellana ha eona le litsamaiso tsa baeloji ho etsa hore e be karolo ea bohlokoa liprothokholong tse fapaneng tsa laboratori.
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Letswai la 4-Nitrophenyl phosphate di(tris) CAS:68189-42-4
Letswai la 4-Nitrophenyl phosphate di(tris) ke motsoako wa dikhemikhale o sebediswang dipatlisisong tsa dikhemikhale le dihlahlobong. Hangata le sebediswa e le substrate bakeng sa diteko tsa alkaline phosphatase enzyme. Letswai lena, ha le hydrolyzed ke alkaline phosphatase, le hlahisa sehlahisoa se mmala o mosehla se ka lekanngwang habonolo ka spectrophotometric. Karabelo ena e sebediswa haholo dikopong tse fapaneng, ho kenyeletswa diteko tsa immunosorbent tse amanang le enzyme (ELISAs) le ho lemoha mesebetsi ya phosphatase. Matla a mmala o mosehla a lekana ka ho toba le mosebetsi wa enzyme, e leng se dumellang ho lekanya ka nepo maemo a alkaline phosphatase disampoleng tsa baeloji. Letswai la 4-Nitrophenyl phosphate di(tris) le tsitsitse, le qhibiliha metsing, mme le na le kutlo e matla haholo, e leng se etsang hore e be kgetho e tsebahalang bakeng sa diteko tsa enzyme dipatlisisong tsa baeloji le tsa bongaka.
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Nitrotetrazolium blue chloride CAS:298-83-9
Nitrotetrazolium blue chloride, e tsejoang hape e le NBT kapa NBT-Cl, ke motsoako oa lik'hemik'hale o sebelisoang haholo litekong tsa baeloji le tsa biochemical. Ke phofo e kristale e mosehla o moputsoa e qhibilihang ka metsing.
NBT e sebediswa kgafetsa e le sesupo sa ho lemoha boteng ba di-radical tsa superoxide kapa di-enzyme tse di hlahisang. E fetoha mmala ha e fokotswa ke di-radical tsa superoxide, e fetoha ho tloha bosehla ho ya ho boputswa bo tebileng ba formazan. Karabelo ena hangata e sebediswa ditekong tsa ho ithuta kgatello ya oxidative, ho hema ha sele, le mosebetsi wa di-enzyme.
NBT e atisa ho sebelisoa mekhoeng e fapaneng, joalo ka ho fokotsa nitroblue tetrazolium, ho bona tlhahiso ea superoxide liseleng le liseleng. E boetse e sebelisitsoe ho immunohistochemistry le histochemistry ho lemoha le ho fumana li-enzyme tse ling le likarolo tsa sele.
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NSP-AS CAS:211106-69-3 Theko ea moetsi
Letswai la kahare la 3-[9-((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate ke molek'hule e rarahaneng e nang le karolo ea acridinium e hoketsoeng letswai la kahare la propanesulfonate. E na le sehlopha sa carboxypropyl, sehlopha sa 4-Methxylphenylsulfonyl, le sehlopha sa amine. Motswako ona ho ka etsahala hore o entswe bakeng sa ditshebediso tse itseng dibakeng tse kang k'hemistri, meriana, kapa dipatlisiso.
Ho feta moo, NAC e bontšitse tšepiso ea ho tšehetsa bophelo bo botle ba sebete ka ho thusa ho tlosa lintho tse chefo, tse kang acetaminophen, e leng moriana o tloaelehileng oa bohloko. E ka boela ea ba le litlamorao tse sireletsang khahlanong le tšenyo ea sebete e bakoang ke ho noa joala.
Ntle le thepa ea eona ea antioxidant le tšehetso ea ho hema, NAC e 'nile ea hlahlojoa bakeng sa melemo ea eona e ka bang teng bophelong ba kelello. Lipatlisiso tse ling li bontša hore e ka ba le tšusumetso e ntle mathateng a maikutlo, a kang khatello ea maikutlo le bothata ba ho qobella ho hema (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3
L-Leucine p-nitroanilide hydrochloride ke motsoako oa lik'hemik'hale o leng sehlopheng sa li-derivatives tsa p-nitroanilide. Ke phofo ea kristale e tšoeu ho isa ho e tšoeu e sebelisoang hangata lipatlisisong tsa biochemical le litekong tsa enzyme.
Hangata L-Leucine p-nitroanilide hydrochloride e sebediswa e le substrate ho lekanya tshebetso ya dienzyme tse kang diprotheine le di-peptidase. Ha dienzyme tsena di arola L-Leucine p-nitroanilide hydrochloride, e lokolla p-nitroaniline, e ka bonwang ka spectrophotometric. Sekgahla sa phetoho ya mmala se tsamaellana ka ho toba le mosebetsi wa enzymatic, e leng se dumellang ho lekanngwa ha mosebetsi wa enzymatic disampoleng tsa baeloji.
Motsoako ona o qhibiliha ka metsing 'me o ka qhibiliha habonolo litharollong tse loketseng tsa buffer bakeng sa tšebeliso litekong tsa enzymatic. Ho bohlokoa ho boloka L-Leucine p-nitroanilide hydrochloride sebakeng se pholileng le se omileng ho boloka botsitso ba eona.
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Guanidine Thiocyanate Sulfate CAS:593-84-0
Guanidine thiocyanate sulfate ke motsoako oa lik'hemik'hale o nkiloeng ho li-ion tsa guanidine, thiocyanate, le sulfate. E sebelisoa hangata lipatlisisong tsa baeloji ea limolek'hule le biochemistry e le moemeli oa chaotropic.
Guanidine thiocyanate sulfate hangata e sebediswa diprotheineng tsa ho arola DNA le RNA ho sitisa lera la sele le diprotheine tse qhibilihisitsweng, e leng se dumellang ho ntshwa ha di-nucleic acid ka katleho. E thusa ho qhibilihisa DNA le RNA, e nolofalletsang ho arolwa le ho hlwekiswa ha tsona disampoleng tse fapaneng tsa baeloji.
Ka lebaka la thepa ea eona ea chaotropic, guanidine thiocyanate sulfate e thusa ho roba khokahano ea haedrojene le likamano tse ling tse seng tsa covalent, e nolofalletsa karohano ea li-nucleic acid ho liprotheine, lipids le likarolo tse ling tsa sele. E boetse e thusa ho thibela ts'ebetso ea RNase, ho netefatsa botšepehi le bohloeki ba li-nucleic acid tse ntšitsoeng..
