8-Anilino-1-naphthalenesulfonic acid CAS: 82-76-8

Probe ea Fluorescent: ANS e sebelisoa haholo e le probe ea fluorescent ho ithuta liphetoho tsa sebopeho sa protheine, lits'ebetso tsa ho mena/ho phutholoha ha protheine, le likamano tsa protheine le ligand. E hokahana le libaka tse sa rateng metsi kapa libaka tse pepesehileng tsa hydrophobic liprotheineng, e leng se fellang ka ho ntšoa ha fluorescence ho ka hlahlojoang ho sebelisoa fluorescence spectroscopy.

Ho lemoha ho se rate metsi: ANS e na le kutloelo-bohloko liphetohong tsa ho se rate metsi tikolohong e nyenyane. E ka sebelisoa ho lemoha le ho lekanya li-surfactants, sesepa sa ho hlatsoa le lintho tse ling tse sa rateng metsi.

Tlhahlobo ea botsitso ba protheine: ANS e sebelisoa hangata litekong tsa botsitso ba mocheso ho lekola botsitso ba protheine. Ka ho beha leihlo matla a fluorescence a liprotheine tse tlamiloeng ke ANS tlas'a mocheso o fapaneng, hoa khoneha ho fumana litšobotsi tsa ho fetoha ha mocheso le mocheso o qhibilihang (Tm) oa protheine.

Lithuto tsa ho kopanya protheine: ANS e ka sebelisoa ho ithuta mekhoa ea ho kopanya protheine, joalo ka ho thehoa ha li-fibril tsa amyloid. Dae e na le kutloelo-bohloko libakeng tse pepesehileng tsa hydrophobic lihlopheng tsa protheine, e leng se etsang hore e be sesebelisoa sa bohlokoa sa ho shebella tsoelo-pele le kinetics ea ho kopanya.

Tlhaloso ea likamano tsa protheine le protheine: ANS e sebelisitsoe ho batlisisa likamano tsa protheine le protheine. Ha liprotheine li sebelisana, ho ka ba le phetoho ho hydrophobicity ea tsona, e ka hlahlojoang ho sebelisoa khanya ea ANS.

Ho sibolloa le ntshetsopele ya meriana: ANS e ka sebediswa ho hlahlobisiseng ba ka bang teng ba ka bang teng bakeng sa meriana. E ka thusa ho lekola kamano e tlamang le tshebedisano ya dimolek'hule tse nyane le diprotheine tse rerilweng, e leng se dumellang bafuputsi ho hlwaya metswako e ka fetolang tshebetso ya protheine.