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Ninhydrine hydrate CAS: 485-47-2 Theko ea moetsi
Ninhydrine hydrate ke motsoako oa lik'hemik'hale o sebelisoang haholo tlhahlobong ea forensic le k'hemistri ea lintho tse phelang. Ke phofo ea kristale e se nang 'mala kapa e mosehla o bobebe e qhibilihang ka metsing le li-solvents tsa lintho tse phelang.
Ninhydrin hydrate e tsebahala ka bokgoni ba yona ba ho arabela le di-amino acid le di-amine tsa mantlha, e leng se hlahisang mmala o bonahalang wa pherese-putswa. Karabelo ena e sebediswa hangata tlhahlobong ya menoana ya forensic ho bona le ho ntlafatsa ponahalo ya menoana e patehileng hodima dibaka tse nang le masoba. Tharollo ya ninhydrin hangata e fafatswa hodima bokahodimo, mme menoana ya menwana e a hola ha nako e ntse e ya ha ninhydrin e arabela le di-amino acid tse teng masalleng a menoana ya menwana.
K'hemistring ea lintho tse phelang, ninhydrin hydrate e sebelisoa e le reagent bakeng sa ho lemoha le ho sekaseka li-amino acid, li-peptide le liprotheine. E ka sebelisoa ho fumana boteng le ho lekanya bongata ba li-amino acid sampole. Karabelo le ninhydrin e fella ka ho thehoa ha sehlahisoa se mebala, se ka lekanyetsoang ka spectrophotometric ho hakanya mahloriso a analyte.
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D-Luciferin CAS: 2591-17-5 Theko ea moetsi
D-Luciferin ke motsoako o monyane o ntšang khanya o fumanehang haholo-holo linthong tse phelang tse kang li-fireflies, libaktheria le lintho tse phelang metsing. Ke karolo ea bohlokoa ea karabelo ea luciferase, ts'ebetso ea biochemical e hlahisang leseli.
Hangata D-Luciferin e sebelisoa e le substrate litekong tsa bioluminescence le mekhoa ea ho nka litšoantšo, moo karabelo ea eona le luciferase e hlahisang leseli le ka bonoang le ho lekanngoa. Sena se etsa hore e be sesebelisoa sa bohlokoa libakeng tse fapaneng tsa lipatlisiso, ho kenyeletsoa baeloji ea limolek'hule, ho sibolloa ha lithethefatsi le ho nka litšoantšo tsa bongaka.
Ka lebaka la thepa ea eona e ikhethang ea mahlo, D-Luciferin e sebelisitsoe haholo lithutong tse kenyeletsang tlhahiso ea liphatsa tsa lefutso, tšebelisano ea protheine le protheine, le lipontšo tsa sele. E fana ka mokhoa o sa hlaseleng le o bonolo oa ho batlisisa lits'ebetso tsa baeloji ka nako ea sebele.
Ho phaella moo, di-derivative tsa D-Luciferin le di-analogue di se di hlahisitswe, tse dumellang diphetoho le ditshebediso tse fapaneng. Di-derivative tsena tse fetotsweng di nolofalletsa maqhubu a fapaneng a kganya, botsitso bo ntlafetseng, le ho kenella ha dinama tse nyane ho ntlafetseng.
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asiti ea p-hydroxybenzoic, monosodium CAS:114-63-6
p-Hydroxybenzoic acid, monosodium (e tsejoang hape e le sodium 4-hydroxybenzoate) ke motsoako oa lik'hemik'hale o nang le foromo ea molek'hule ea C7H5NaO3. Ke letsoai la sodium le nkiloeng ho p-hydroxybenzoic acid, e leng motsoako oa tlhaho oa lintho tse phelang o fumanoang limela tse kang litholoana le meroho.
p-Hydroxybenzoic acid, monosodium, e sebelisoa liindastering tse fapaneng ka lebaka la thepa ea eona ea ho loantša likokoana-hloko. E sebelisoa hangata e le sesebelisoa sa ho boloka lintho tse kotsi lijong, lino, litlolong le lihlahisoa tsa tlhokomelo ea botho ho thibela kholo ea libaktheria, li-fungus le tomoso. E thusa ho lelefatsa bophelo ba lihlahisoa tsena ka ho thibela ho senyeha le ho boloka boleng ba tsona.
Litšebelisong tsa meriana, p-Hydroxybenzoic acid, monosodium li sebelisoa e le setlatsetsi merianeng e nooang le mefuteng ea letlalo. Litšobotsi tsa eona tsa likokoana-hloko li thusa ho boloka botsitso le ho se bolaee likokoana-hloko tsa lihlahisoa tsena, ho netefatsa polokeho le katleho ea tsona.
Ho phaella moo, p-Hydroxybenzoic acid, monosodium e na le thepa ea antioxidant, e etsang hore e be molemo ho thibeleng tšenyo ea oxidative lihlahisoa tse fapaneng. E ka thusa ho sireletsa khahlanong le ho senyeha ha metsoako le ho atolosa ho tšoarella ha sehlahisoa ka kakaretso..
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4-Nitrophenyl phosphate disodium letsoai hexahydrate CAS:4264-83-9
4-Nitrophenyl phosphate disodium salt hexahydrate ke motsoako oa lik'hemik'hale o sebelisoang hangata e le substrate bakeng sa ho lemoha ts'ebetso ea li-enzyme tsa phosphatase. O hlaha e le phofo e tšoeu ho isa ho e tšoeu 'me o qhibiliha haholo ka metsing. Ha o sebelisoa ke li-enzyme tsa phosphatase, o feta karabelong e fellang ka tlhahiso ea 'mala o mosehla, o ka lekanyetsoang ka spectrophotometric. Motsoako ona o fumana lits'ebetso litekong tse fapaneng tsa biochemical le lisebelisoa tsa tlhahlobo bakeng sa ho lemoha le ho lekanya ts'ebetso ea phosphatase lisampoleng..
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Methylphenazinium methosulfate CAS:299-11-6
Methylphenazinium methosulfate (MPMS) ke motsoako o sebetsang ka redox o atisang ho sebelisoa e le mojari oa lielektrone lithutong tse fapaneng tsa biochemical le biophysical. Ke letsoai le nang le methylphenazinium cation (motsoako oa heterocyclic) le methosulfate anion.
Hangata MPMS e sebelisoa e le mokhoa o mong oa ho jara lielektrone tsa setso, joalo ka ferricyanide kapa phenazine ethosulfate, ka lebaka la botsitso ba eona le ho qhibiliha ho hoholo metsing. E na le thepa e ntle ea redox, e e lumellang ho amohela le ho fetisetsa lielektrone nakong ea liketso tsa enzymatic.
E 'ngoe ea lits'ebetso tsa bohlokoa tsa MPMS ke litekong tse kenyeletsang ho lekanya phetiso ea lielektrone kapa ts'ebetso ea enzymatic. E sebelisoa khafetsa hammoho le sistimi ea enzyme ho beha leihlo phetiso ea lielektrone lipakeng tsa likarolo tse fapaneng. Phokotso ea MPMS e ka bonoa ka spectrophotometric, moo monya oa eona o fetohang ka lebaka la lits'ebetso tsa phetiso ea lielektrone.
MPMS e boetse e sebelisoa lithutong tse amanang le ho hema ha mitochondrial le phosphorylation ea oxidative. E ka sebetsa e le moamoheli oa elektronike oa maiketsetso, e lumellang bafuputsi ho batlisisa ts'ebetso le taolo ea lits'ebetso tsena litsamaisong tse fapaneng tsa baeloji.
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4-Nitrophenyl-alpha-L-fucopyranoside CAS:10231-84-2
4-Nitrophenyl-alpha-L-fucopyranoside ke motsoako oa lik'hemik'hale o leng oa lelapa la li-glycoside. E entsoe ka molek'hule ea tsoekere ea fucose e hoketsoeng sehlopheng sa 4-nitrophenyl. Motsoako ona o sebelisoa hangata e le substrate litekong tsa enzymatic ho ithuta ts'ebetso ea li-fucosidase, e leng li-enzyme tse amehang ho heleheng ha limolek'hule tse nang le fucose. Ha e sebetsoa ke enzyme ea fucosidase, 4-nitrophenyl-alpha-L-fucopyranoside ea petsoha, e leng se fellang ka ho lokolloa ha 4-nitrophenol, e ka lekanngoang ka bongata ho sebelisoa spectrophotometry. Substrate ena e na le thuso haholo lithutong tse amanang le ts'ebetso ea enzymatic, ho khetheha ha substrate, tlhahlobo ea li-inhibitor, le kinetics ea li-enzyme tsa fucosidase.
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N-Ethylmaleimide CAS:128-53-0 Theko ea moetsi
N-Ethylmaleimide (NEM) ke motsoako o monyane wa tlhaho o sebediswang haholo dipatlisisong tsa biochemistry le baeloji ya dimolek'hule. E sebetsa e le thibelo e ikgethang ya dihlopha tsa protheine sulfhydryl (thiol) ka ho fetola le ho thibela tshebetso ya tsona ka tsela e ke keng ya fetolwa. NEM e arabela haholo dihlopheng tsa sulfhydryl, tse kang tse fumanwang ho amino acid cysteine, mme e ka arabela ka dihlopha tsa free sulfhydryl le tse ka hara diprotheine. Sena se etsa hore NEM e be sesebediswa se molemo bakeng sa ho ithuta tshebetso ya protheine, dikamano tsa protheine le protheine, le mosebetsi wa enzyme. Dithoto tsa yona tsa thibelo di sebedisitswe dikopong tse fapaneng, ho kenyeletswa proteomics, enzymology, biology ya sebopeho, le ho sibolla dithethefatsi.
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5-Sulfosalicylic acid dihydrate CAS: 5965-83-3
5-Sulfosalicylic acid dihydrate ke motsoako oa lik'hemik'hale o sebelisoang haholo lilaboratoring tsa biochemical le tsa meriana. Ke ntho e tšoeu ea kristale e qhibilihang haholo metsing. 5-Sulfosalicylic acid dihydrate ke sehlahisoa sa salicylic acid 'me se na le foromo ea limolek'hule ea C7H6O6S. Hangata e sebelisoa e le reagent bakeng sa pula ea protheine 'me e ka sebelisoa ho lekanya liprotheine lisampoleng tsa baeloji, joalo ka moroto le serum. Ho feta moo, e ka sebelisoa ho fumana lintho tse fapaneng, ho kenyeletsoa li-nucleic acid, li-enzyme, lihormone le lithethefatsi. Sebopeho sa dihydrate sa motsoako se bontša hore se na le limolek'hule tse peli tsa metsi ka molek'hule ea asiti.
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Potassium iodide CAS: 7681-11-0
Potassium iodide (KI) ke motsoako o sa pheleng o nang le di-cation tsa potassium (K+) le di-anion tsa iodide (I-). Ke ntho e tiileng e tshweu, e kang kristale e qhibilihang haholo metsing. Potassium iodide e na le tshebediso le ditshebediso tse fapaneng ka lebaka la thepa ya yona.
E 'ngoe ea tšebeliso e ka sehloohong ea potassium iodide ke meriana. E atisa ho sebelisoa e le tlatsetso ho phekola le ho thibela maemo a amanang le khaello ea iodine, joalo ka goiter, ho se leka-lekane ha lihormone tsa thyroid, le mefuta e itseng ea kankere ea thyroid. E ka boela ea sebelisoa e le phekolo ea tšohanyetso bakeng sa ho pepesehela mahlaseli a kotsi, kaha e thusa ho thibela ho monngoa ha iodine ea mahlaseli a kotsi ke tšoelesa ea thyroid.
Iodide ea potassium e boetse e na le ts'ebeliso libakeng tsa laboratori. E sebelisoa e le reagent k'hemistring ea tlhahlobo ho lemoha boteng ba likarolo tse itseng, tse kang loto le mercury, ka ho thehoa ha li-precipitate tse mosehla tse sa qhibiliheng. Ho feta moo, e ka sebelisoa e le mohloli oa li-ion tsa iodide liketsong tse fapaneng tsa lik'hemik'hale.
Lefapheng la ho pheha, iodide ea potassium ka linako tse ling e eketsoa letsoai la tafole (letsoai le nang le iodized) e le mokhoa oa ho thibela khaello ea iodine ho baahi. Letsoai le nang le iodized le jeoa ke batho ba bangata lefatšeng ka bophara e le mohloli oa lijo oa iodine.
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8-Anilino-1-naphthalenesulfonic acid letswai la ammonium CAS:28836-03-5
8-Anilino-1-naphthalenesulfonic acid Letswai la ammonium ke motsoako oa lik'hemik'hale o sebelisoang hangata e le dae e khanyang. E na le sebopeho se entsoeng ka sehlopha sa aniline se hoketsoeng molek'huleng ea naphthalenesulfonic acid e nang le letsoai la ammonium. Motsoako ona o qhibiliha ka metsing 'me o bontša ho monngoa le ho ntšoa ho matla khanyeng e bonahalang.
Ka lebaka la thepa ea eona ea fluorescent, letsoai la ammonium la 8-Anilino-1-naphthalenesulfonic acid hangata le sebelisoa e le probe kapa sesupo lithutong tse fapaneng tsa biochemical le biophysical. Le ka sebelisoa ho lemoha liphetoho pH, liphetoho tsa sebopeho liprotheineng, likamano tsa protheine le ligand, le boteng ba li-ion tse itseng.
Ha e thabisoa ke bolelele ba leqhubu bo loketseng ba khanya, motsoako ona o ntša khanya e putsoa e khanyang, e leng se lumellang ho bonoa le ho lekanngoa habonolo. Litšobotsi tsa eona tsa khanya li etsa hore e be sesebelisoa sa bohlokoa libakeng tsa lipatlisiso tse kang baeloji ea limolek'hule, biochemistry le baeloji ea sele.
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Sodium dodecyl sulfate CAS:151-21-3
Sodium dodecyl sulfate (SDS) ke anionic surfactant e sebediswang haholo e sebediswang diindastering tse fapaneng le dikopong tsa dipatlisiso. Ke ntho e tiileng e tshweu kapa phofo e qhibilihang metsing mme e etsa foam e matla, e tsitsitseng ha e sisinyeha. SDS e tsebahala ka bokgoni ba yona ba ho qhibilihisa le ho ntsha diprotheine, e leng se etsang hore e be molemo mekgweng ya ho ntsha protheine, ho e hlwekisa le ho e sebedisa ka electrophoresis. Ho feta moo, SDS e sebediswa haholo e le sesepa sa ho hlwekisa dihlahisweng tsa lapeng le tsa tlhokomelo ya motho ka mong, hammoho le ditshebedisong tsa ho hlwekisa diindasteri. Hape ke motsoako wa bohlokwa ditekong tse ngata tsa biochemical le tsa baeloji ya dimolek'hule, moo e sebediswang bakeng sa lysis ya sele, ho ikarola ha DNA, le jwalo ka moemedi wa ho ntsha nature ho gel electrophoresis.
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BCA-2K CAS:207124-63-8 Theko ea moetsi
BCA-2K e emetse Beta-Carotene Apocarotenoid-2-Ketolase. Ke enzyme e bapalang karolo ea bohlokoa tlhahisong ea mebala ea limela e bitsoang apocarotenoids. Mebala ena e nkiloe molek'huleng ea pele ho beta-carotene 'me e ameha lits'ebetsong tse fapaneng tsa baeloji limela.
BCA-2K e susumetsa ka ho khetheha phetoho ea beta-carotene hore e be li-apocarotenoid tse itseng ka ho eketsa sehlopha sa ketone ho molek'hule. Karabelo ena ea enzymatic e bohlokoa bakeng sa tlhahiso ea li-apocarotenoid tse itseng tse nang le mesebetsi le mesebetsi e fapaneng ea baeloji limela.
Li-apocarotenoid tse hlahisoang ke BCA-2K li fumanoe li phetha karolo ea nts'etsopele ea limela, likarabelo tsa khatello ea maikutlo, le tšireletso khahlanong le likokoana-hloko le likokoanyana. Li ka boela tsa sebetsa e le limolek'hule tse bontšang lintho tse amehang lits'ebetsong tse fapaneng tsa 'mele.
Thuto ea BCA-2K le karolo ea eona ho etseng apocarotenoid ha e bohlokoa feela bakeng sa ho utloisisa baeloji ea limela empa hape e na le lits'ebetso tse ka bang teng libakeng tse kang ntlafatso ea lijalo, ho ikatisa limela, le nts'etsopele ea lihlahisoa tsa tlhaho tse nang le melemo ea meriana kapa ea phepo e nepahetseng.
