5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt CAS:18656-96-7 Setšoantšo se Hlahisitsoeng

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium letsoai CAS:18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Letswai la cyclohexylammonium, le tsejwang hape e le X-glucuronide, ke motsoako wa dikhemikhale o sebediswang haholo ditekong tsa baeloji ya dimolek'hule le enzymology. Ke substrate ya chromogenic e atisang ho sebediswa ho lemoha mosebetsi wa enzyme beta-D-glucuronidase.

Motswako ona o na le sehlopha sa indole se kopantsoeng le karolo ea glucuronic acid ka tlamo ea glycosidic. Lintho tse nkeloang sebaka ke bromo le chloro li fana ka botsitso le ho ntlafatsa ho qhibiliha ha motsoako.

Ha e futhumatsoa ka sampole e nang le beta-D-glucuronidase, enzyme e kgaola tlamo ya glycosidic, e lokolla karolo ya indole. Karabelo ena e fella ka ho thehwa ha precipitate e putsoa kapa phetoho ya mmala, e leng se dumellang ho lemoha habonolo mosebetsi wa enzyme ka pono kapa ka spectrophotometric.

Ka lebaka la ho ikhetha ha eona bakeng sa beta-D-glucuronidase, X-glucuronide e sebelisoa haholo mekhoeng ea baeloji ea limolek'hule joalo ka liteko tsa phetoho ea baktheria le tomoso, liteko tsa liphatsa tsa lefutso tsa reporter, le ho taka ka histochemical. E fana ka mokhoa o bonolo le o bonolo oa ho lemoha boteng kapa tlhahiso ea beta-D-glucuronidase 'me hangata e sebelisoa hammoho le liteko tse ling tsa liphatsa tsa lefutso kapa tsa biochemical.

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Qaqiso ea Sehlahisoa

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Tšebeliso le Phello

Tšusumetso ea letsoai la 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium (X-glucuronide) ke bokhoni ba lona ba ho hlahisa phetoho e putsoa kapa 'mala boteng ba enzyme beta-D-glucuronidase. Karabelo ena ea enzymatic e etsahala ha tlamo ea glycosidic pakeng tsa sehlopha sa indole le karolo ea glucuronic acid e khaoloa ke beta-D-glucuronidase.

Tšebeliso ea X-glucuronide e etsoa haholo-holo litekong tsa baeloji ea limolek'hule le tsa enzymology. Hangata e sebelisoa e le substrate ho lemoha ts'ebetso ea beta-D-glucuronidase. Litšebeliso tse ling tse ikhethileng li kenyelletsa:

Liteko tsa liphatsa tsa lefutso tsa moqolotsi oa litaba: Hangata X-glucuronide e sebelisoa e le substrate litekong tsa liphatsa tsa lefutso tsa moqolotsi oa litaba moo beta-D-glucuronidase e sebelisoang e le enzyme ea moqolotsi oa litaba. Ka ho kopanya sebaka sa mokhothaletsi kapa tatellano ea khoutu ea liphatsa tsa lefutso tse khahlisang le liphatsa tsa lefutso tsa beta-D-glucuronidase, bafuputsi ba ka lekanya tlhahiso kapa ts'ebetso ea liphatsa tsa lefutso ka ho beha leihlo ho thehoa ha phetoho e putsoa kapa 'mala ha X-glucuronide e eketsoa.

Liteko tsa phetoho ea baktheria le tomoso: X-glucuronide e ka sebelisoa ho lemoha phetoho e atlehileng ea baktheria kapa tomoso ka vector e nang le gene ea beta-D-glucuronidase. Lisele tse fetotsoeng tse hlahisang enzyme li ka hydrolyze X-glucuronide, tsa hlahisa phetoho e bonahalang ea 'mala kapa ho potlaka.

Ho daea ka histochemical: X-glucuronide e sebediswa mekhoeng ya ho daea ka histochemical ho bona boteng le kabo ya tshebetso ya beta-D-glucuronidase dinama kapa diseleng. Ho daea hona ho dumella sebaka le ho hlwaya disele tse hlahisang enzyme.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C26H36BrClN2O7
Teko	99%
Ponahalo	Phofo e tšoeu
Nomoro ea CAS	18656-96-7
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.