Letswai la 4-Nitrophenyl phosphate di(tris) CAS:68189-42-4

Letswai la 4-Nitrophenyl phosphate di(tris) le atisa ho sebediswa e le substrate bakeng sa alkaline phosphatase ditekong tse fapaneng tsa biochemical le enzyme. Ha e hahelletswa ke alkaline phosphatase, e hlahisa sehlahiswa se mmala o mosehla se ka lekanngwang habonolo ka spectrophotometric. Karabelo ena e sebediswa haholo ditshebedisong tse kang diteko tsa immunosorbent tse amanang le enzyme (ELISAs) le ho lemoha tshebetso ya phosphatase.

Matla a 'mala o mosehla a hlahisoang a lekana ka ho toba le ts'ebetso ea enzyme, e leng se lumellang ho lekanya ka nepo maemo a alkaline phosphatase lisampoleng tsa baeloji. Sena se etsa hore e be molemo maemong a bongaka le lipatlisiso bakeng sa ho ithuta ts'ebetso ea alkaline phosphatase liseleng, liseleng le maro a baeloji.

Ho phaella moo, letsoai la 4-Nitrophenyl phosphate di(tris) le tsitsitse ebile le qhibiliha ka metsing haholo, e leng se eketsang ho loketse ha lona bakeng sa maemo a fapaneng a liteko. Kutloisiso ea lona e phahameng le bonolo ba ho lekanya li etsa hore e be khetho e tsebahalang e le substrate litekong tsa enzyme, e thusang tlhahlobong ea kinetics ea enzyme, ts'ebetso ea enzyme le ho sibolloa ha lithethefatsi.