-
Amylase, a-, Aspergillus oryzae CAS:9001-19-8 Theko ea moetsi
Amylase ke enzyme e thusang ho qhaqha starch hore e be tsoekere e bonolo joalo ka maltose le glucose. Hangata e hlahisoa ke lintho tse phelang tse fapaneng, ho kenyeletsoa libaktheria, li-fungus le limela.
“a-” ho “a-amylase” e bolela mofuta oa amylase o hasetsang ka ho khetheha litlamo tsa glycosidic tsa alpha-1,4 tse ka hare ho molek'hule ea starch. Sena se fella ka tlhahiso ea li-polysaccharides tse khutšoane tsa ketane, tse ka aroloang ho feta hore e be glucose ke li-enzyme tse ling tsa amylase.
Aspergillus oryzae ke mofuta oa fungus e nang le filamentous e tsejoang ka bokhoni ba eona ba ho hlahisa amylase e ngata. E sebelisoa hangata lits'ebetsong tsa tlhahiso ea li-enzyme tsa indasteri ka lebaka la ts'ebetso ea eona e matla ea amylolytic. Li-enzyme tse hlahisoang ke Aspergillus oryzae, ho kenyeletsoa le a-amylase, li sebelisoa haholo liindastering tse fapaneng, tse kang tlhahiso ea lijo, ea ho etsa biri, ea masela le ea sesepa.
Indastering ea lijo, a-amylase e nkiloeng ho Aspergillus oryzae hangata e sebelisoa ho bakeng, ho ritela le ho sebetsana le starch. E thusa ho qhaqha starch ka har'a hlama, malt le lisebelisoa tse ling tse tala, e leng se nolofalletsang phetoho ea lik'habohaedreite tse rarahaneng hore e be tsoekere e bonolo e ka fetoloang ke tomoso kapa libaktheria nakong ea ho belisoa. Enzyme ena e boetse e sebelisoa tlhahisong ea masela ho nolofatsa ho tlosoa ha metsoako ea boholo e thehiloeng ho starch masela.
-
Aprotinin CAS: 9087-70-1 Theko ea moetsi
Aprotinin ke sethibela-mafu sa tlhaho se thehiloeng protheineng sa lienzyme tse fapaneng tsa proteolytic, ho kenyeletsoa trypsin, chymotrypsin, le plasmin. E sebelisoa hangata libakeng tsa bongaka, haholo-holo liopereisheneng tsa pelo le methapo ea mali, ho fokotsa ho tsoa mali le ho fokotsa tahlehelo ea mali. Aprotinin e sebetsa ka ho thibela ts'ebetso ea lienzyme tsena, tse amehang ho senyeheng ha maqeba a mali le ho senyeha ha fibrin, protheine e thusang ho thehoeng ha maqeba a mali. Ka ho thibela ts'ebetso e feteletseng ea enzymatic, aprotinin e thusa ho boloka botsitso ba maqeba a mali, ka hona e fokotsa mathata a ho tsoa mali nakong ea opereishene.
-
Theko ea Thrombin CAS: 9002-04-4
Thrombin ke enzyme ea bohlokoa e amehang ho hoama ha mali, e tsejoang hape e le ho hoama ha mali. E hlahisoa ho tsoa ho protheine ea eona ea pele ho mali, prothrombin, e le karabelo ea kotsi kapa tšenyo ea methapo ea mali. Thrombin e bapala karolo ea bohlokoa ho thehoeng ha ho hoama ha mali, e thusang ho thibela ho tsoa mali ho feteletseng le ho khothalletsa pholiso ea maqeba.
Hang ha e se e kentswe tshebetsong, thrombin e fetola fibrinogen, protheine e nngwe e ka hara madi, hore e be fibrin. Fibrin e bopa sebopeho se kang letlooeng se tshwarang diplatelete le disele tse kgubedu tsa madi, e leng se etsang hore madi a dule a tiile. Thrombin e boetse e kenya tshebetsong dintho tse ding tse etsang hore madi a dule a qhibidihile, e leng se eketsang tshebetso ya ho qhibidiha ha madi.
Ntle le karolo ea eona ho theheng ha maqeba a mali, thrombin e na le mesebetsi e meng ea bohlokoa. E khothalletsa pholiso ea maqeba ka ho susumetsa ho falla le ho ata ha lisele tse amehang tokisong ea lisele. Thrombin e boetse e sebetsa e le vasoconstrictor, e bakang hore methapo ea mali e tšesaane le ho fokotsa phallo ea mali ho ea sebakeng seo kotsi e leng ho sona.
-
Urease CAS: 9002-13-5 Theko ea moetsi
Urease ke enzyme e susumetsang ho haelloa ha urea hore e be ammonia le carbon dioxide. E hlahisoa ke likokoana-hloko tse fapaneng, ho kenyeletsoa libaktheria, li-fungus le limela. Urease e bohlokoa bakeng sa metabolism ea naetrojene 'me e bapala karolo ea bohlokoa lits'ebetsong tse fapaneng tsa baeloji, joalo ka ho haelloa ha urea liphiong le ho monngoa ha naetrojene limela. E boetse e na le lits'ebetso tsa indasteri, ho kenyeletsoa le ho hlahlojoa ha libaktheria tse hlahisang urea, manyolo a thehiloeng ho urea, le ho fumanoa ha lithibela-mafu tsa urea bakeng sa kalafo ea maemo a itseng a bongaka.
-
Leupeptin hemisulfate CAS:103476-89-7
Leupeptin hemisulfate ke thibelo ea protease e sebelisoang haholo lipatlisisong tsa biochemical le tsa bongaka. E nkiloe libaktheria tsa Streptomyces 'me e sebetsa ka ho thibela ts'ebetso ea liprotheine tse fapaneng, ho kenyeletsoa trypsin, chymotrypsin le plasmin.
Leupeptin hemisulfate hangata e sebediswa ditekong tsa laboratori ho thibela ho senyeha ha protheine ho sa batleheng, kaha diprotheine di ka senya diprotheine le dipeptide, e leng se amang diphetho tsa diteko. Ka ho thibela tshebetso ya protease, leupeptin hemisulfate e thusa ho tsitsisa diprotheine le ho boloka botsitso ba tsona nakong ya diteko.
Ho phaella moo, leupeptin hemisulfate e sebelisitsoe lithutong tsa setso sa sele ho batlisisa mesebetsi ea li-protease lits'ebetsong tse fapaneng tsa sele, tse kang ho supa lisele, ho apoptosis le ho fetoha ha protheine.
-
Lipase e tsoang ho pancreas ea kolobe CAS:9001-62-1
Lipase ke enzyme e susumetsang ho haelloa ha mafura, haholo-holo ho senyeha ha triglycerides hore e be mafura a acid le glycerol. E hlahisoa ke lintho tse phelang tse fapaneng, ho kenyeletsoa liphoofolo, limela le likokoana-hloko. Lipase e phetha karolo ea bohlokoa tšilong ea lijo, kaha e thusa ho heletsa le ho monya mafura a lijong ka maleng a manyenyane. Ho feta moo, lipase e na le lits'ebetso tse ngata liindastering tse kang ts'ebetso ea lijo, tlhahiso ea sesepa, meriana le tlhahiso ea biofuel. Bokhoni ba eona ba ho haelloa ke mafura bo etsa hore e be ea bohlokoa bakeng sa mesebetsi e kang ho fetola mafura, ho ntlafatsa tatso le ho ntša oli.
-
Lysostaphine CAS:9011-93-2 Theko ea moetsi
Lysostaphin ke enzyme e matla ea likokoana-hloko e hlaselang le ho bolaea Staphylococcus aureus ka ho khetheha, baktheria e ikarabellang bakeng sa tšoaetso e fapaneng bathong. E nkiloe ho baktheria ea Staphylococcus simulans 'me e na le bokhoni ba ho heletsa lebota la sele la S. aureus, e leng se lebisang timetsong ea eona.
Enzyme ena e shebane ka ho khetheha lera la peptidoglycan la lerako la sele ea baktheria, e sitisa sebopeho sa eona le ho baka lysis ea baktheria. Mokhoa ona o ikhethang oa ts'ebetso o etsa hore lysostaphin e sebetse hantle haholo khahlanong le mefuta ea S. aureus, ho kenyeletsoa le tse hanyetsanang le lithibela-mafu tse sebelisoang hangata.
-
L-Lactate dehydrogenase CAS:9001-60-9
L-Lactate dehydrogenase (LDH) ke enzyme e bapalang karolo ea bohlokoa metabolism ea matla a sele, haholo-holo phetohong ea pyruvate ho lactate. E susumetsa phetoho e ka fetoloang ea L-lactate ho pyruvate, e sebelisa NAD+ e le cofactor.
LDH e fumanoa liseleng tse fapaneng tsa 'mele, ho kenyeletsoa sebete, pelo, mesifa le lisele tse khubelu tsa mali. Maemo a eona a ts'ebetso a ka sebetsa e le letšoao la tlhahlobo ea mafu a itseng, a kang tšenyo ea sebete, lefu la pelo le mefuta e meng ea mofetše.
Mosebetsi o ka sehloohong oa LDH ke ho boloka botsitso ba matla a sele ka ho nolofatsa phetoho ea pyruvate, sehlahisoa sa glycolysis, ho ba lactate. Ts'ebetso ena, e tsejoang e le anaerobic glycolysis, e etsahala ha oksijene e le sieo 'me e lumella lisele ho tsoela pele ho hlahisa matla ha phepelo ea oksijene e lekanyelitsoe.
-
Pepsin CAS:9001-76-7 Theko ea moetsi
Pepsin ke enzyme ea tšilo ea lijo e fumanoang ka mpeng e bapalang karolo ea bohlokoa tšilong ea lijo le ho qhala ha liprotheine. Ke ea sehlopha sa li-enzyme tse bitsoang li-protease, tse ikarabellang bakeng sa ho qhaqha liprotheine hore e be li-peptide tse nyane le li-amino acid, e leng se lumellang ho monngoa ha tsona ka maleng a manyenyane.
Pepsin e hlahisoa le ho ntšoa ke lisele tse ka hare ho mpa, tse tsejoang e le lisele tse ka sehloohong, ka mokhoa o sa sebetseng o bitsoang pepsinogen. Ha lijo li kena ka mpeng, li baka ho lokolloa ha asiti ea mpa, e fetolang pepsinogen hore e be mofuta oa eona o sebetsang, pepsin. Pepsin e sebetsa hantle tikolohong e nang le asiti, kaha pH e tlase ea mpa e thusa ho kenya tšebetsong le ho boloka ts'ebetso ea eona ea enzymatic.
-
Protheine Kinase Supplement KT5823 CAS:126643-37-6
Tlatsetso ea protheine kinase KT5823 ke thibelo e nyane ea molek'hule e shebaneng le le ho thibela ts'ebetso ea protheine kinase ka ho khetheha. Li-protein kinase ke li-enzyme tse fosforolang liprotheine ka ho eketsa lihlopha tsa phosphate ho masala a itseng a amino acid, hangata serine, threonine, kapa tyrosine. Ts'ebetso ena ea phosphorylation e bapala karolo ea bohlokoa litseleng tse fapaneng tsa lipontšo tsa sele, ho laola ts'ebetso ea protheine, le ho laola lits'ebetso tsa sele tse kang kholo ea sele, karohano, phapang le apoptosis.
KT5823 e sebediswa hangata diphuputsong tsa dipatlisiso ho fuputsa tshebetso le ditsela tsa ho bontsha dipontsho tsa di-protein kinase. Ka ho thibela di-protein kinase, KT5823 e ka thusa bafuputsi ho fumana diprotheine le ditsela tse itseng tse amehang ke mosebetsi wa kinase. Tlhahisoleseding ena e ka ba le temohisiso ho utlwisiseng karolo ya di-protein kinase mesebetsing ya sele le ho tsebahatsa dipheo tse ntjha tsa kalafo.
-
Pyridoxal-5′-phosphate monohydrate CAS:41468-25-1
Pyridoxal-5′-phosphate monohydrate, e tsejoang hape e le PLP, ke mofuta o sebetsang oa vithamine B6. Ke coenzyme ea bohlokoa e amehang liketsong tse fapaneng tsa enzymatic 'meleng. PLP e bapala karolo ea bohlokoa ho metabolism ea amino acid, tlhahiso ea neurotransmitter, le tlhahiso ea heme, karolo ea hemoglobin.
PLP e sebetsa e le coenzyme ka ho tlama dienzyme tse itseng le ho thusa dikarabelong tsa dikhemikhale. E sebetsa e le catalyst, e thusa ho fetola dintho tse fapaneng, tse kang di-amino acid, hore e be dihlahiswa tse fapaneng. PLP e bohlokwa haholo ho metabolism ya di-amino acid, jwalo ka ho fetola tryptophan hore e be serotonin, kapa ho senyeha ha glycine hore e be serine.
Ntle le karolo ea eona ho metabolism ea amino acid, PLP e boetse e ameha ho qaptjoeng ha li-neurotransmitter tse kang dopamine, norepinephrine, le gamma-aminobutyric acid (GABA). Li-neurotransmitter tsena li bohlokoa bakeng sa ts'ebetso e nepahetseng ea boko 'me li ameha taolong ea maikutlo, kutloisisong, le phetisong ea matšoao lipakeng tsa lisele tsa methapo.
Ho feta moo, PLP ea hlokahala bakeng sa ho etsoa ha heme, e leng karolo ea hemoglobin e fumanoang liseleng tse khubelu tsa mali. Hemoglobin e ikarabella bakeng sa ho jara oksijene ho pholletsa le 'mele, e leng se etsang hore PLP e be ea bohlokoa bakeng sa ho boloka maemo a tloaelehileng a oksijene le ho netefatsa ts'ebetso e nepahetseng ea litho le lisele tse fapaneng.
Ka lebaka la bohlokoa ba eona lits'ebetsong tse ngata tsa biochemical, tlatsetso ea PLP ka linako tse ling e khothaletsoa batho ba nang le maemo a itseng a bongaka kapa mefokolo e amang metabolism ea vithamine B6. E fumaneha ka mokhoa oa tlatsetso 'me e ka laeloa ke bafani ba tlhokomelo ea bophelo bakeng sa litlhoko tse itseng.
-
Reductase, glutathione CAS:9001-48-3
Glutathione reductase ke enzyme e bapalang karolo ea bohlokoa ho bolokeng botsitso ba glutathione e fokotsehileng le e nang le oxidized (GSH/GSSG) liseleng. Glutathione ke tripeptide e entsoeng ka li-amino acid glycine, cysteine, le glutamate. E sebetsa e le antioxidant e kholo ea sele 'me e ameha lits'ebetsong tse fapaneng tsa sele, ho kenyeletsoa ho hloekisa lisele, ho hlophisoa le ho lokisoa ha DNA, le ho hlophisoa ha protheine.
Glutathione reductase e thusa ho fokotsa glutathione e nang le oxidized (GSSG) ho ba sebopehong sa yona se fokotsehileng (GSH) ka ho sebedisa NADPH (nicotinamide adenine dinucleotide phosphate) e le cofactor. Karabelo ena e bohlokwa bakeng sa ho boloka boemo bo fokotsehileng ba glutathione diseleng, kaha GSSG e ka bokellana ha ho na le kgatello ya oxidative.
Ka ho boloka karolelano e nepahetseng ea GSH ho GSSG, glutathione reductase e thusa ho sireletsa lisele tšenyong ea oxidative e bakoang ke mefuta ea oksijene e arabelang (ROS) le limolek'hule tse ling tse kotsi. E sebetsa e le sebapali sa bohlokoa tsamaisong ea tšireletso ea li-antioxidant ea lisele.
Ho feta moo, glutathione reductase e boetse e ameha ho etseng hape glutathione disulfide (GSSG) e hlahisoang nakong ea lits'ebetso tse fapaneng tsa metabolic, ho netefatsa hore ho na le glutathione e fokotsehileng (GSH) bakeng sa mesebetsi ea eona e mengata ea sele.
