-
Heppso sodium CAS: 89648-37-3 Theko ea moetsi
Letswai la sodium la N-[2-Hydroxyethyl]piperazine-N'-[2-hydroxypropanesulfonic acid] ke motsoako oa lik'hemik'hale o nang le foromo ea C8H19N2NaO4S. Ke letsoai la sodium le tsoang ho piperazine, le nang le lihlopha tse sebetsang tsa hydroxyethyl le hydroxypropanesulfonic acid. Hangata le sebelisoa indastering ea meriana e le moemeli oa buffering le stabilizer mefuteng ea meriana. Motsoako ona o thusa ho boloka pH le botsitso ba meriana.
-
P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7
P-Nitrophenyl beta-D-lactopyranoside, e tsejoang hape e le PNPG, ke motsoako o atisang ho sebelisoa litekong tsa enzymatic ho lekanya mosebetsi oa beta-galactosidase, e leng enzyme e amehang metabolism ea lik'habohaedreite. PNPG ke substrate ea maiketsetso e ka aroloang ke beta-galactosidase, e leng se fellang ka ho lokolloa ha sehlahisoa se 'mala o mosehla. Bongata ba hydrolysis ea substrate bo ka lekanngoa ka spectrophotometric ka ho lekanya ho monngoa ha sehlahisoa ka bolelele bo itseng ba leqhubu. Sena se lumella bafuputsi ho lekola mosebetsi le kinetics ea beta-galactosidase maemong a fapaneng, joalo ka ho ithuta mosebetsi oa enzyme, ho hlahloba li-inhibitor tsa enzyme kapa li-activator, kapa ho lekola liphello tsa liphetoho mosebetsing oa enzyme.
-
popso sesquisodium CAS:108321-08-0
Letswai la sesquisodium la Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium, le tsejoang hape e le letswai la sesquisodium la PIPES, ke motsoako wa dikhemikhale o sebediswang e le sesebelisoa sa ho thibela diphetoho tse fapaneng tsa saense le tsa indasteri. Le thusa ho boloka boemo bo tsitsitseng ba pH ditharollong, haholoholo mofuteng wa pH ya mmele. Letswai la sesquisodium la PIPES le sebediswa hangata mefuteng ya setso sa sele, biochemistry ya protheine, electrophoresis, mekgwa ya baeloji ya dimolek'hule, le ditsamaiso tsa ho fana ka dithethefatsi. Le sebetsa jwalo ka taolo ya pH le ho ntlafatsa tshebetso ya enzyme le botsitso, e leng se etsang hore e be ya bohlokwa dipatlisisong tse ngata le ditsamaisong tsa indasteri.
-
ACES CAS: 7365-82-4 Theko ea moetsi
N-(2-Acetamido)-2-aminoethanesulfonic acid, e tsejoang hape e le ACES, ke sesebelisoa sa ho thibela likokoana-hloko se sebelisoang haholo lipatlisisong tsa saense. E thusa ho boloka pH e tsitsitseng litharollong 'me e qhibiliha haholo metsing. ACES e na le chefo e tlase ebile e sitisa lits'ebetso tsa biochemical hanyane, e leng se etsang hore e lokele lits'ebetso tse fapaneng. Hangata e sebelisoa e le sesebelisoa sa ho thibela likokoana-hloko lithutong tsa ho tsitsisa protheine le litekong tsa enzyme, moo e bolokang maemo a matle bakeng sa sebopeho sa protheine le ts'ebetso ea enzyme.
-
Phenylgalactoside CAS:2818-58-8
Phenylgalactoside, e tsejoang hape e le p-nitrophenyl β-D-galactopyranoside (pNPG), ke substrate ea maiketsetso e sebelisoang khafetsa litekong tsa baeloji ea lik'hemik'hale le ea limolek'hule. E sebelisoa hangata ho lemoha le ho lekanya ts'ebetso ea enzyme β-galactosidase.
Ha phenylgalactoside e haelloa ke β-galactosidase, e ntša p-nitrophenol, e leng motsoako o mosehla. Tokoloho ea p-nitrophenol e ka lekanngoa ka bongata ho sebelisoa spectrophotometer, kaha ho monngoa ha p-nitrophenol ho ka bonoa ka bolelele ba 405 nm.
-
DIPSO CAS: 68399-80-4 Theko ea moetsi
DIPSO e emetse “Diisopropyl azodicarboxylate,” e leng reagent e sebediswang haholo k'hemistring ya tlhaho. E sebediswa haholo-holo karabelong ya Mitsunobu, e leng mokgwa wa ho fetola dino tse tahang hore e be dihlopha tse fapaneng tse sebetsang jwalo ka di-ester, di-ether, kapa di-amine. DIPSO e sebetsa e le mohlodi wa ntho e arabelang haholo e bitswang azodicarboxylate, e thusang phetoho ena.
-
4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
4-Nitrophenyl-beta-D-glucopyranoside ke substrate e sebediswang haholo ditekong tsa biochemical ho lekola tshebetso ya dienzyme tse kang β-glucuronidase. Motswako ona o hydrolyzed ke enzyme, e leng se fellang ka ho lokollwa ha 4-nitrophenol, e ka lekanngwang ho sebediswa spectrophotometry. Tshebediso ya wona e thusa bafuputsi ho ithuta dikarolo tse fapaneng tsa metabolism ya dithethefatsi, chefo, le tlhahlobo ya bongaka e amanang le dikarabelo tsa glucuronidation.
-
Tris maleate CAS: 72200-76-1
Tris maleate ke motsoako oa lik'hemik'hale o sebetsang e le sesebelisoa sa ho thibela pH le ho lokisa liindasteri tse fapaneng. E sebelisetsoa ho boloka maemo a pH a tsitsitseng le ho hanyetsa liphetoho tse bakoang ke ho eketsoa ha li-acid kapa li-base. Tris maleate e sebelisoa hangata lipatlisisong tsa biochemical, tlhoekiso ea protheine, lits'ebetso tsa indasteri le k'hemistri ea tlhahlobo. E sebetsa haholo-holo ho thibela pH maemong a tlase 'me e tsebahala ka ho feto-fetoha ha eona ho boloka maemo a pH a matle ka ho fetisisa.
-
MOBS CAS:115724-21-5 Theko ea moetsi
4-morpholin-4-ylbutane-1-sulfonic acid, e tsejoang hape e le MOBS, ke motsoako oa tlhaho o entsoeng ka maiketsetso o sebelisoang e le moemeli oa buffering lipatlisisong tsa baeloji le tsa biochemical. E tsitsitse 'me e ka boloka pH e tsitsitseng ho tloha ho e sa nke lehlakore ho isa ho e nang le alkaline e nyane.BS e sebelisoa haholo temong ea sele, litekong tsa enzyme, mekhoa ea baeloji ea limolek'hule le electrophoresis. E lumellana le mefuta e fapaneng ea li-reagent 'me e tsebahala ka botsitso ba eona le ho hanyetsa liphetoho mochesong le pH.
-
1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose CAS:4163-59-1
1,2,3,4,6-penta-O-acetyl-alpha-D-galactopyranose ke motsoako oa lik'hemik'hale o leng oa lelapa la lik'habohaedreite. Ke motsoako oa alpha-D-galactopyranose, tsoekere e hlahang ka tlhaho. Motsoako ona o ikhethileng o na le lihlopha tse hlano tsa acetyl tse hoketsoeng lihlopha tse itseng tsa hydroxyl molek'huleng ea tsoekere. E sebelisoa hangata lits'ebetsong tse fapaneng tsa lik'hemik'hale le meriana, ho kenyeletsoa le e le thepa ea ho qala bakeng sa ho kopanya metsoako e meng. Sebopeho sa eona sa acetylated se ntlafatsa botsitso ba eona le ho sebetsa hape, e leng se etsang hore e be motheo o thusang oa k'hemistri ea lintho tse phelang.
-
POPSO CAS: 68189-43-5 Theko ea moetsi
POPSO, e khutsufalitsoeng bakeng sa letsoai la sesquisodium la Piperazine-N,N'-bis(2-hydroxypropanesulfonic acid) sesquisodium, ke sesebelisoa sa ho thibela se sebelisoang haholo lipatlisisong tsa baeloji le tsa biochemical. E thusa ho boloka boemo bo tsitsitseng ba pH litharollong, haholo-holo ka har'a mefuta ea pH ea 'mele. Letsoai la sesquisodium la PIPES le sebelisoa moetlong oa sele, biochemistry ea protheine, electrophoresis, mekhoa ea baeloji ea limolek'hule, litsamaiso tsa ho fana ka meriana, le tse ling. Bokhoni ba eona ba ho laola pH bo e etsa sesebelisoa sa bohlokoa lipatlisisong tse fapaneng le lits'ebetsong tsa indasteri.
-
BES CAS: 10191-18-1 Theko ea moetsi
N,N-Bis(hydroxyethyl)-2-aminoethanesulfonic acid, e tsejoang hape e le BES kapa N,N-Bis(2-hydroxyethyl)aminoethanesulfonic acid, ke motsoako oa lik'hemik'hale o sebelisoang hangata e le moemeli oa buffering lits'ebetsong tse fapaneng tsa saense le tsa indasteri.
BES ke motsoako oa zwitterionic, ho bolelang hore e na le litefiso tse ntle le tse mpe ka har'a sebopeho sa eona. Thepa ena e e lumella ho boloka pH e tsitsitseng ka katleho litharollong.
BES e na le boleng ba pKa ba hoo e ka bang 7.4, e leng se etsang hore e be molemo haholo bakeng sa ho thibela maemo a pH a fisioloji. Hangata e sebelisoa litekong tsa baeloji le tsa biochemical, tse kang tlhoekiso ea protheine, liketso tsa enzyme, le setso sa sele, moo ho boloka pH e itseng ho leng bohlokoa.
Ho phaella moo, BES e sebediswa haholo mekgweng ya electrophoresis, kaha e thusa ho boloka pH e hlokahalang bakeng sa karohano le tlhahlobo ya di-biomolecule tse tjhajisitsweng, tse kang diprotheine le di-nucleic acid.
