P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Ho lemoha ts'ebetso ea beta-galactosidase: PNPG e sebelisoa hangata litekong ho lekanya ts'ebetso ea beta-galactosidase, e leng enzyme e susumetsang hydrolysis ea lactose ho ba glucose le galactose. Hydrolysis ea PNPG ke beta-galactosidase e lokolla molek'hule ea p-nitrophenol (pNP), e ka bonoang ka spectrophotometric ka lebaka la 'mala oa eona o mosehla.

Ho hlahloba li-inhibitor tsa enzyme le li-activator: PNPG e ka sebelisoa tlhahlobong ea ho sebelisa matla a mangata ho khetholla metsoako e fetolang ts'ebetso ea beta-galactosidase. Ka ho lekanya sekhahla sa hydrolysis ea PNPG boteng ba metsoako e fapaneng ea liteko, bafuputsi ba ka khetholla li-inhibitor tse fokotsang ts'ebetso ea enzyme kapa li-activator tse ntlafatsang ts'ebetso ea enzyme.

Thuto ea kinetics ea enzyme: Hydrolysis ea PNPG ke beta-galactosidase e latela kinetics ea Michaelis-Menten, e lumellang bafuputsi ho fumana liparamente tsa bohlokoa tsa enzyme tse kang lebelo le phahameng la karabelo (Vmax) le Michaelis constant (Km). Tlhahisoleseling ena e thusa ho utloisisa kamano ea substrate ea enzyme le katleho ea catalytic.

Ditshebediso tsa baeloji ya dimolek'hule: Beta-galactosidase, e kgaolang PNPG, e sebediswa hangata e le jini ya moqolotsi wa ditaba baelojing ya dimolek'hule. Karolo e ka tlase ya PNPG hangata e sebediswa ho lemoha le ho bona pontsho ya jini ya moqolotsi wa ditaba, e fanang ka tsela e bonolo le e utlwahalang ya ho lekola pontsho ya jini ditsamaisong tse fapaneng tsa diteko.