4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Teko ea ts'ebetso ea enzyme: Hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside ke lienzyme tse kang β-glucosidase e ka sebelisoa ho lekanya ts'ebetso ea enzyme. Ho lokolloa ha 4-nitrophenol ho ka lekanngoa ho sebelisoa spectrophotometry, e lumellang ho fumana kinetics ea enzyme le lithuto tsa thibelo.

Tlhahlobo ea liphatsa tsa lefutso le liteko tsa moqolotsi oa litaba: 4-Nitrophenyl-beta-D-glucopyranoside e ka kopanngoa le li-tag tse itseng tsa limolek'hule tse kenyellelitsoeng liprotheineng tsa fusion. Mosebetsi oa enzyme oa protheine ea fusion o ka lekanngoa habonolo ka ho lekanya ho lokolloa ha 4-nitrophenol, ho fana ka temohisiso mabapi le tlhahiso ea liphatsa tsa lefutso le tšebelisano-'moho ea protheine le protheine.

Ho sibolloa ha meriana le tlhahlobo ea phallo e phahameng: Hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside e ka sebelisoa e le teko ea ts'ebetso e 'ngoe bakeng sa ho sibolloa ha meriana. E lumella tlhahlobo e potlakileng ea lilaebrari tse kholo tsa metsoako ho khetholla li-enzyme tse ka bang teng kapa li-activator.

Teko ea tlhahlobo: Mafu a mang a amahanngoa le liphetoho mesebetsing e itseng ea enzyme. Ka ho sebelisa hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside ke lienzyme tse itseng e le teko ea tlhahlobo, mafu ana a ka fumanoa kapa a hlokomeloa. Sena se kenyelletsa maemo a amanang le mathata a polokelo ea lysosomal le mafu a sebete.