Phenylgalactoside CAS:2818-58-8

Tšusumetso mosebetsing oa enzyme: Phenylgalactoside e sebelisoa hangata ho lekanya mosebetsi oa enzyme β-galactosidase. Ha phenylgalactoside e haelloa ke hydrolyzed ke β-galactosidase, e ntša p-nitrophenol. Ho bokellana ha p-nitrophenol ho ka lekanngoa ka bongata, ho fana ka temohisiso mabapi le mosebetsi oa β-galactosidase. Tšusumetso ena e sebelisoa lits'ebetsong tse kang liteko tsa enzyme le litsamaiso tsa tlhahlobo.

Tlhahlobo ea tlhahiso ea liphatsa tsa lefutso: Phenylgalactoside hangata e sebelisoa e le substrate litekong tsa baeloji ea limolek'hule ho ithuta tlhahiso ea liphatsa tsa lefutso. Jini ea lacZ, e kenyang β-galactosidase, hangata e kopanngoa le tatellano ea taolo ea liphatsa tse ling tsa lefutso tse khahlisang. Tlhaloso ea liphatsa tsa lefutso tsa lacZ le hydrolysis ea phenylgalactoside ke β-galactosidase li ka bontša mokhoa oa tlhahiso le boemo ba liphatsa tsa lefutso tse ithutoang.

Mekhoa ea ho hlahloba: Phenylgalactoside ke karolo ea bohlokoa ea mekhoa ea ho hlahloba e sebelisang ts'ebetso ea β-galactosidase. Mohlala o mong o tsebahalang haholo ke mokhoa oa ho hlahloba o moputsoa-o mosoeu, o sebelisoang ho khetholla lisele tse kopantsoeng kapa tse fetotsoeng litekong tsa baeloji ea limolek'hule. Likolone tse nkileng DNA e kopantsoeng ka katleho kapa tse fetotsoeng liphatsa tsa lefutso li tla hlahisa β-galactosidase, e leng se lebisang ho hydrolysis ea phenylgalactoside le ho thehoa ha 'mala o moputsoa.

Tlhoekiso ea protheine: Maemong a mang, phenylgalactoside e ka sebelisoa e le ligand bakeng sa chromatography ea affinity ho hloekisa liprotheine tse tlamang ka ho khetheha kapa tse kengoang tšebetsong ke β-galactosidase. Protheine e khahlisang e ka ba le tag ea affinity kapa tag ea fusion e nang le sebaka se tlamang sa β-galactosidase. Ka ho fetisa motsoako oa protheine ka har'a kholomo e nang le phenylgalactoside e sa sisinyeheng, protheine e lakatsehang e ka bolokoa ka khetho 'me hamorao ea hlakoloa.