NBD chloride CAS: 10199-89-0

NBD chloride, e tsejoang hape e le 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, ke dae e fluorescent e sebelisoang haholo lits'ebetsong tsa lik'hemik'hale le tsa baeloji. Ke ea sehlopha sa li-derivatives tsa nitrobenzoxadiazole (NBD), tse bontšang thepa e matla ea fluorescence.

Kloride ea NBD e khetholloa ka bokhoni ba eona ba ho theha maqhama a covalent le li-nucleophile, tse kang li-amine tsa mantlha, li-thiol, le lihlopha tsa hydroxyl. Thepa ena e e lumella ho sebelisoa e le moemeli oa ho ngola mabitso bakeng sa li-biomolecule, ho kenyeletsoa liprotheine, li-peptide, li-nucleic acid le limolek'hule tse ling tse nyane.

Ha NBD chloride e arabela le nucleophile, e ba le karabelo e nkeloang sebaka, e leng se fellang ka ho thehwa ha sehlahisoa se tsitsitseng se nang le leibole ea NBD. Sehlahisoa sena se nang le leibole se bontša tlhahiso e matla ea fluorescence, e leng se etsang hore e be molemo bakeng sa mekhoa e fapaneng ea ho lemoha le ho etsa litšoantšo.

Ho ntšoa ha khanya ea limolek'hule tse nang le leibole ea NBD ho ka tsosoa ke khanya ea ultraviolet (UV) hoo e ka bang 470 nm, 'me khanya e ntšoang hangata e bonoa hoo e ka bang 530 nm. Leqhubu lena la maqhubu a ho ntšoa ha khanya le oela sebakeng se setala sa spectrum e bonahalang, moo tšitiso ea ho lemoha ho tsoa lisampoleng tsa baeloji e leng tlase haholo.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Ho ngolwa ha di-covalent: NBD chloride e ka theha maqhama a covalent le dihlopha tsa nucleophilic ka di-biomolecule, tse kang di-amine tsa mantlha, di-thiol, le dihlopha tsa hydroxyl. Sena se dumella ho ngolwa ha di-biomolecule tsena ka ho kgetheha bakeng sa merero ya ho lemoha le ho etsa ditshwantsho.

Ntlafatso ea khanya: Ha NBD chloride e arabela le li-nucleophiles, e etsa sehlahisoa se nang le leibole ea NBD e khanyang. Sehlahisoa sena se nang le leibole se bontša tlhahiso e matla ea khanya, e leng se etsang hore e be molemo bakeng sa ho bona le ho latela limolek'hule tse nang le leibole.

Ho ba le kutlo le ho totobala: Ho ntsha ha fluorescence ha dihlahiswa tse ngotsweng ka leibole ya NBD ho bonolo haholo, ho dumella ho lemoha bongata bo tlase ba dimolek'hule tse ngotsweng. Ho feta moo, ho thehwa ha tlamo ya covalent pakeng tsa NBD chloride le di-nucleophile ho fana ka ho totobala, kaha ho netefatsa ho hokela dae ho tsitsitseng le ho ikgethang ho biomolecule e rerilweng.

Ho ngolwa ha dimolekhule: NBD chloride e ka sebediswa ho ngolwa dimolekhule tse fapaneng tsa dimolekhule, ho kenyeletswa diprotheine, dipeptide, di-nucleic acid, le dimolekhule tse ding tse nyane. Sena se nolofalletsa bafuputsi ho batlisisa sebaka, matla le dikamano tsa dimolekhule tsena tsa dimolekhule tsamaisong ya baeloji.

Microscopy ea Fluorescence: Li-biomolecule tse nang le leibole ea NBD li ka bonoa ka ho sebelisa microscopy ea fluorescence. Mokhoa ona o lumella ho shebella limolek'hule tse nang le leibole boemong ba sele kapa ba subcellular, ho fana ka tlhahisoleseling ea bohlokoa mabapi le kabo le boitšoaro ba tsona.

Cytometry ea phallo: Li-biomolecule tse nang le leibole ea NBD li ka hlahlojoa le ho lekanngoa ho sebelisoa cytometry ea phallo. Mokhoa ona o lumella tlhahlobo e phahameng ea lisele kapa likaroloana tse nang le leibole, e fanang ka tlhahisoleseling mabapi le maemo a tlhahiso kapa thepa ea bokaholimo ba li-biomolecule tse nang le leibole.

Ho ngolwa le ho latedisa protheine: NBD chloride e ka sebediswa ho ngolwa masalla a itseng a amino acid kapa dihlopha tse sebetsang diprotheineng. Sena se nolofalletsa bafuputsi ho ithuta sebopeho sa protheine, ho mena, matla le dikamano ka nako ya sebele.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C6H2ClN3O3
Teko	99%
Ponahalo	Phofo e mosehla
Nomoro ea CAS	10199-89-0
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.