TRIS-Acetate CAS: 6850-28-8 Theko ea moetsi

Tris-acetate (TRIS-Acetate) ke sesebelisoa se sebelisoang haholo litekong tsa baeloji le tsa biochemical. E na le motsoako oa tris(hydroxymethyl)aminomethane (Tris) le acetic acid, e sebetsang e le taolo ea pH le stabilizer. PH ea sesebelisoa sa TRIS-Acetate hangata e tloha ho 7.4 ho isa ho 8.4.
Tšusumetso e kholo ea TRIS-Acetate ke ho boloka pH e tsitsitseng, e leng ea bohlokoa bakeng sa liketso tse ngata tsa baeloji le tsa biochemical. E sebetsa e le buffer ka ho fokotsa liphetoho life kapa life tse kholo pH tse ka hlahang ka lebaka la li-acid kapa metheo e eketsehileng nakong ea mekhoa ea liteko.
TRIS-Acetate e fumana lits'ebetso tse fapaneng baelojing ea limolek'hule, biochemistry le biotechnology:
Electrophoresis ea DNA le RNA: TRIS-Acetate e sebelisoa hangata e le sesebelisoa se sebetsang sa ho thibela ho electrophoresis ea agarose le polyacrylamide gel. E fana ka tikoloho e tsitsitseng ea pH nakong ea karohano ea likaroloana tsa DNA le RNA ho latela boholo ba tsona.
Tlhahlobo ea Protheine: Li-buffer tsa TRIS-Acetate li sebelisoa bakeng sa electrophoresis ea protheine, joalo ka SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). E netefatsa botsitso le karohano ea protheine nakong ea ts'ebetso.
Likarabelo tsa Enzyme: Li-buffer tsa TRIS-Acetate li sebelisoa khafetsa litekong le lithutong tsa enzyme. E fana ka mefuta e metle ea pH bakeng sa likarabelo tse fapaneng tsa enzymatic 'me e thusa ho boloka ts'ebetso ea enzyme.
Temo ea Lisele le Lisele: Li-buffer tsa TRIS-Acetate li sebelisoa mecheng ea temo ea lisele ho boloka pH e loketseng bakeng sa kholo le ho ata ha lisele. E thusa ho boloka maemo a 'mele a hlokahalang bakeng sa ho phela ha sele.