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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
2-Nitrophenyl-beta-D-glucopyranoside ke motsoako oa lik'hemik'hale o nang le molek'hule oa glucopyranoside o kopantsoeng le sehlopha sa nitrophenyl.Hangata e sebelisoa e le karoloana litlhahlobong tsa enzymatic ho bona le ho lekanya tšebetso ea li-enzyme tse kang beta-glucosidase.Sehlopha sa nitrophenyl se ka phunyeletsoa ke enzyme, e leng se etsang hore ho lokolloe sehlahisoa sa 'mala o mosehla se ka lekanngoang spectrophotometrically.Motsoako ona o bohlokoa haholo ho ithuteng kinetics ea enzyme le tlhahlobo e phahameng ea li-enzyme inhibitors kapa activator.E boetse e sebelisoa lipatlisisong tsa biochemical bakeng sa lipatlisiso tsa metabolism ea carbohydrate hape e le karoloana e ikhethileng ea glycosidic-linkage-specific.
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Tris Base CAS: 77-86-1 Theko ea Moetsi
Tris Base, eo hape e tsejoang e le Tromethamine kapa THAM, ke motsoako oa lintho tse phelang tse sebelisoang hangata lefapheng la biochemistry le biology ea limolek'hule.Ke phofo e tšoeu, e kristale e qhibilihang haholo metsing 'me e na le monko o monate oa amine.Hangata Tris Base e sebelisoa e le sesebelisoa sa ho thibela pH ho boloka pH e tsitsitseng litekong le mekhoa e fapaneng ea likokoana-hloko, tse kang DNA le lithuto tsa protheine.E ka boela ea sebelisoa ha ho etsoa meriana le ho hlahisa li-agent tse sebetsang ka holim'a metsi.Ka kakaretso, Tris Base ke karolo ea bohlokoa lits'ebetsong tse ngata tsa laboratori moo ho boloka pH e nepahetseng ho leng bohlokoa.
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4-Morpholineethanesulfonic acid CAS: 4432-31-9
4-Morpholineethanesulfonic acid, eo hangata e tsejoang e le MES, ke motsoako oa zwitterionic o sebeletsang e le sesebelisoa se thibelang lipatlisiso tsa bioloji le lik'hemik'hale.E thusa ho boloka pH e tsitsitseng ho pota 6-7.5 mme e sebelisoa haholo ka lebaka la chefo ea eona e tlaase le ho lumellana le mekhoa e sa tšoaneng ea likokoana-hloko le li-enzyme.MES e sebelisoa ho electrophoresis, lithuto tsa enzyme, setso sa lisele, tlhoekiso ea protheine, le mekhoa e meng ea liteko e hlokang taolo e nepahetseng ea pH.
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4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
4-Nitrophenyl-beta-D-glucopyranoside ke substrate e atisang ho sebelisoa litekong tsa lik'hemik'hale ho hlahloba mosebetsi oa li-enzyme tse kang β-glucuronidase.Motsoako ona o na le hydrolyzed ke enzyme, e hlahisang ho lokolloa ha 4-nitrophenol, e ka lekanyetsoang ho sebelisa spectrophotometry.Tšebeliso ea eona e thusa bafuputsi ho ithuta likarolo tse fapaneng tsa metabolism ea lithethefatsi, toxicology, le tlhahlobo ea bongaka e amanang le karabelo ea glucuronidation.
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MOPSO letsoai la sodium CAS: 79803-73-9
MOPSO sodium letsoai ke motsoako oa lik'hemik'hale o tsoang ho MOPS (3-(N-morpholino)propanesulfonic acid).Ke letsoai la zwitterionic buffer, ho bolelang hore e na le tefiso e ntle le e mpe, e e lumellang ho boloka botsitso ba pH ka nepo litekong tse fapaneng tsa baeloji le lik'hemik'hale.
Mofuta oa letsoai oa sodium oa MOPSO o fana ka melemo e kang ho ntlafatsa ho qhibiliha ka har'a litharollo tsa metsi, ho etsa hore ho be bonolo ho sebetsana le ho itokisetsa.E sebelisoa hangata joalo ka sesebelisoa sa "buffering" mecheng ea litaba tsa setso sa lisele, mekhoa ea baeloji ea limolek'hule, tlhahlobo ea protheine le karabelo ea enzyme.
Letsoai la sodium la MOPSO le thusa ho boloka pH ea sebaka sa kholo ho setso sa lisele, ho fana ka tikoloho e tsitsitseng bakeng sa kholo ea lisele le ts'ebetso.Ka mekhoa ea baeloji ea limolek'hule, e tsitsisa pH ea metsoako ea karabelo le li-buffers tse tsamaisang, e netefatsa liphetho tse nepahetseng le tse tšepahalang ho DNA le RNA isolation, PCR, le gel electrophoresis.
E boetse e sebelisoa tlhahlobong ea liprotheine, e sebetsa joalo ka ntho e sireletsang nakong ea tlhoekiso ea protheine, quantification, le electrophoresis.Letsoai la sodium la MOPSO le netefatsa maemo a nepahetseng a pH bakeng sa botsitso ba protheine le ts'ebetso ho pholletsa le lits'ebetso tsena.
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4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4
4-Methylumbelliferyl-beta-D-glucopyranoside ke substrate e atisang ho sebelisoa litlhahlobong tsa enzymatic ho ithuta mosebetsi oa li-enzyme tsa beta-glucosidase.Ha e sebelisoa ke beta-glucosidase, e kenella ka hydrolysis, e leng se fellang ka ho lokolloa ha 4-methylumbelliferon, e ka fumanoang le ho lekanyetsoa ka ho sebelisa fluorescence spectroscopy.Motsoako ona o sebelisoa haholo mafapheng a biochemistry, biology ea limolek'hule, le biotechnology bakeng sa liteko tsa ts'ebetso ea enzyme le merero ea tlhahlobo.Thepa ea eona ea fluorescence e etsa hore e be bonolo haholo ebile e loketse lits'ebetso tse phahameng.
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METHYL Beta-D-GLUCOPYRANOSIDE HEMIHYDRATE CAS:7000-27-3
Methyl beta-D-glucopyranoside hemihydrate ke motsoako oa lik'hemik'hale oo e leng oa sehlopha sa glucopyranosides.Ke phofo e tšoeu ea kristale e qhibilihang ka metsing.Motsoako ona o sebelisoa hangata joalo ka mohloli oa lik'habohaedreite mecheng ea litaba tsa setso sa lisele le karoloana ea karabelo ea enzymatic lipatlisisong tsa biochemical le biotechnological.E ka sebetsa e le mohlala oa ho ithuta metabolism ea carbohydrate, lipalangoang le ts'ebeliso lits'ebetsong tse fapaneng tsa tlhaho.Methyl beta-D-glucopyranoside hemihydrate e fumana lits'ebetso lefapheng la glycobiology, enzymology, le nts'etsopele ea lithethefatsi, moo e sebelisoang e le motsoako oa lisebelisoa bakeng sa liteko le liteko tse fapaneng.
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AMPSO CAS: 68399-79-1 Theko ea Moetsi
AMPSO, kapa 3-[(1,1-dimethyl-2-hydroxyethyl) amino] -2-hydroxypropanesulfonic acid, ke zwitterionic buffer e atisang ho sebelisoa liphuputsong tsa baeloji le biochemical.E na le boleng ba pKa ba hoo e ka bang 7.9, e leng se etsang hore e tšoanelehe bakeng sa ho boloka maemo a tsitsitseng a pH maemong a sa tšoaneng a liteko.AMPSO e atisa ho sebelisoa mecheng ea litaba tsa setso sa lisele, ho hloekisa liprotheine, litlhahlobo tsa li-enzyme, li-gel electrophoresis, le DNA sequencing.E thusa ho boloka pH e lakatsehang, ho netefatsa maemo a nepahetseng bakeng sa kholo ea lisele, botsitso ba protheine, ts'ebetso ea enzyme, le karohano e nepahetseng le tlhahlobo ea li-biomolecules.Ka bokhoni ba eona ba ho hanyetsa liphetoho tsa pH tse bakoang ke ho eketsoa ha li-acids kapa metheo, AMPSO ke sesebelisoa sa bohlokoa ho ho boloka taolo e nepahetseng ea pH mefuteng e mengata ea liteko tsa baeloji le tsa biochemical.
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Bis-tris hydrochloride CAS: 124763-51-5
Bis-tris hydrochloride ke motsoako o nang le thepa ea ho thibela hangata e sebelisoang litekong tsa biochemical le tsa baeloji.E thusa ho boloka pH e tsitsitseng mme e sebelisoa ho protheine electrophoresis, liteko tsa ts'ebetso ea enzyme, setso sa lisele, le litlhahlobo tsa meriana.Mosebetsi oa eona o ka sehloohong ke ho hanela liphetoho ho pH ha li-acid kapa li-base li kenngoa tharollong, e leng se etsang hore e be sesebelisoa sa bohlokoa lits'ebetsong tse fapaneng tsa saense le tsa indasteri.
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Alpha-D-Glucose pentaacetate CAS: 3891-59-6
Alpha-D-glucose pentaacetate ke motsoako oa lik'hemik'hale o fumanoang ka acetylating lihlopha tsa hydroxyl tsa alpha-D-glucose ka lihlopha tse hlano tsa acetyl.Hangata e sebelisoa ho organic synthesis e le sehlopha se sireletsang bakeng sa lihlopha tsa hydroxyl tse teng ka lik'habohaedreite.E ka boela ea sebelisoa e le motsoako oa litšupiso lipatlisisong le tlhahlobong ea lik'hemik'hale, hape e le ntho e qalang bakeng sa ho kopanya metsoako e fapaneng.Ho feta moo, glucose pentaacetate e 'nile ea etsoa lipatlisiso bakeng sa ts'ebeliso ea eona e ka bang teng lits'ebetsong tsa phano ea lithethefatsi, ka lebaka la thepa ea eona ea tokollo e laoloang.
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AMPD CAS: 115-69-5 Theko ea Moetsi
2-Amino-2-methyl-1,3-propanediol, e tsejoang hape e le AMPD kapa α-methyl serinol, ke motsoako oa lik'hemik'hale o nang le mokhoa oa molek'hule oa C4H11NO2.Ke joala ea amino e sebelisoang ka tloaelo e le lik'hemik'hale tse lipakeng tsa motsoako oa meriana le metsoako ea tlhaho.AMPD e tsejoa ka bokhoni ba eona ba ho sebetsa e le mothusi oa chiral ka ho itšoara ka mokhoa o sa tsitsang, ho etsa hore e be ea bohlokoa tlhahisong ea metsoako e hloekileng ea enantiomerically.Ho feta moo, e 'nile ea sebelisoa e le motsoako oa tlhokomelo ea botho le lihlahisoa tsa litlolo bakeng sa thepa ea eona ea mongobo.
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CAPS SODIUM LETSWAI CAS:105140-23-6
CAPS sodium letsoai ke zwitterionic buffer e sebelisoang hangata lits'ebetsong tsa biochemical le molecular biology.E na le boleng ba pKa ba hoo e ka bang 10.4, e etsa hore e sebetse bakeng sa mefuta ea pH e pakeng tsa 9.7 le 11.1.Letsoai la sodium la CAPS le sebelisoa ho protheine electrophoresis, karabelo ea enzymatic, litlhahlobo tsa baeloji le lik'hemik'hale, le mecha ea litaba ea setso sa lisele.E fana ka khanyetso ho liphetoho tsa pH tse bakoang ke litšila 'me e na le ho qhibiliha hantle metsing.
