4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Tšusumetso ea 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ke ho sebetsa e le substrate bakeng sa enzyme beta-glucosidase. Enzyme ena e arola tlamo ea glucosidase ea MUG, e leng se fellang ka ho lokolloa ha 4-Methylumbelliferone (4-MU). Tšebeliso ea MUG e haholo-holo lefapheng la baeloji, haholo-holo bakeng sa ho lemoha le ho khetholla libaktheria tse hlahisang beta-glucosidase. MUG e sebelisoa haholo ho fumaneng Escherichia coli (E. coli) metsing le lisampoleng tsa lijo. E. coli e na le enzyme beta-glucosidase, e ka etsang hore MUG e haelloe ke metsi 'me ea hlahisa lets'oao la fluorescent moo ho nang le khanya ea ultraviolet (UV). Thepa ea fluorescent ea 4-MU e lumella ho fumanoa le ho lekanngoa habonolo. Ha substrate ea MUG e haelloa ke metsi, 4-MU e hlahisoang e ntša fluorescence e putsoa, ​​​​e nolofalletsang ho khetholla libaktheria tse nang le ts'ebetso ea beta-glucosidase. Mokhoa ona o atisa ho sebelisoa ho lekola tikoloho le ho lekola polokeho ea lijo, kaha o fana ka mokhoa o potlakileng le o bonolo oa ho lemoha tšilafalo ea baktheria. Ntle le ts'ebeliso ea oona ho microbiology, MUG e ka boela ea sebelisoa ho ithuta ts'ebetso le thibelo ea beta-glucosidase lipatlisisong tsa biochemistry le baeloji ea limolek'hule. Phatsimo ea eona e nolofalletsa ho lekanya kinetics ea enzyme 'me e ka sebelisoa ho hlahloba li-inhibitor kapa li-activator tse ka bang teng tsa ts'ebetso ea beta-glucosidase. Ka kakaretso, MUG ke motsoako o feto-fetohang o fumanang ts'ebeliso e pharaletseng masimong a microbiology, biochemistry le baeloji ea limolek'hule bakeng sa ho lemoha ts'ebetso ea beta-glucosidase le ho tsebahatsa libaktheria tse hlahisang enzyme ena.