2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Substrate ea Enzyme: ONPG e sebelisoa hangata e le substrate bakeng sa beta-galactosidase, e leng enzyme e hydrolyzes ONPG ho hlahisa motsoako o mosehla (o-nitrophenol) o ka bonoang habonolo ka spectrophotometric. Karabelo ena ea enzymatic e ka sebelisoa ho lekanya mosebetsi oa beta-galactosidase, e leng se etsang hore ONPG e be sesebelisoa sa bohlokoa lithutong tsa enzymology.

Liteko tsa baeloji ea limolek'hule: ONPG e sebelisoa e le substrate litekong tse fapaneng tsa baeloji ea limolek'hule, haholo-holo litekong tsa liphatsa tsa lefutso tsa beta-galactosidase. Litekong tsena, substrate e thehiloeng ho ONPG e sebelisoa ho lekanya ts'ebetso ea gene ea moqolotsi, eo hangata e laoloang ke tatellano e itseng ea thahasello ea moqolotsi. Ts'ebetso ea beta-galactosidase, e bontšitsoeng ke phetoho ea 'mala e hlahisoang ke hydrolysis ea ONPG, e fana ka tlhahisoleseling ea bohlokoa mabapi le ts'ebetso ea moqolotsi.

Tlhahlobo ea tlhahiso ea liphatsa tsa lefutso: ONPG e boetse e sebelisoa tlhahlobong ea tlhahiso ea liphatsa tsa lefutso. Ka ho hokahanya tatellano ea thahasello ea promoter le gene ea beta-galactosidase, bafuputsi ba ka lekanya ts'ebetso ea beta-galactosidase ba sebelisa ONPG e le substrate. Boemo ba ts'ebetso ea beta-galactosidase bo bonahatsa matla le ts'ebetso ea promoter, e leng se lumellang tlhahlobo ea maemo a tlhahiso ea liphatsa tsa lefutso.

Ditshebediso tsa tlhahlobo: ONPG e ka sebediswa ditshebedisong tsa tlhahlobo, haholoholo ho fumaneng dikokwana-hloko tse bakang pathogenic. Dibaktheria tse fapaneng, tse kang Escherichia coli le mefuta e itseng ya Shigella le Salmonella, di hlahisa beta-galactosidase e ka kgaolang ONPG. Karabelo ena ya hydrolysis e hlahisa phetoho e bonahalang ya mmala, e ka sebediswang ho fumana boteng ba dibaktheria tsena disampoleng tsa bongaka.