4-Morpholineethanesulfonic acid CAS: 4432-31-9

Ho thibela pH: MES e na le boleng ba pKa bo ka bang 6.1, e leng se etsang hore e be buffer e sebetsang hantle ho pH e pakeng tsa 5.5 le 6.7. E thusa ho boloka pH e tsitsitseng ka ho hanela diphetoho tsa asiti kapa alkalinity. Sena se thusa haholo ditekong le ditekong tse hlokang tikoloho e itseng ya pH.

Lithuto tsa Enzyme: MES e sebelisoa hangata lipatlisisong tsa enzyme le litekong ka lebaka la ho lumellana ha eona le lienzyme tse fapaneng. E thusa ho boloka maemo a pH a matle bakeng sa ts'ebetso ea enzyme, ho netefatsa liphetho tse nepahetseng le tse tšepahalang.

Ho Hloekisa Protheine: MES e sebelisoa lits'ebetsong tsa ho hloekisa protheine, joalo ka chromatography, ho boloka botsitso le ts'ebetso ea protheine e reriloeng. E thusa ho boloka sebopeho le ts'ebetso ea protheine nakong ea mehato ea ho hloekisa.

Electrophoresis: MES e sebelisoa khafetsa lits'ebetsong tsa gel electrophoresis, haholo-holo bakeng sa ho arola le ho sekaseka liprotheine tse nyane le li-peptide. Bokhoni ba eona ba ho boloka liprotheine bo tiisa pH e tsitsitseng, e leng ea bohlokoa bakeng sa pono e nepahetseng le tlhaloso ea lihlopha tsa protheine.

Temo ea Lisele: MES e sebelisoa hangata lithutong tsa temo ea lisele le mefuteng ea mecha ea phatlalatso e le sesebelisoa sa ho thibela. E thusa ho boloka pH e le ka har'a sebaka se loketseng bakeng sa kholo ea lisele, ho phela hantle le lits'ebetso tsa biochemical ntle le ho sitisa mesebetsi ea sele.

Likarabelo tsa Lik'hemik'hale: MES e ka boela ea sebelisoa e le reagent liketsong tsa lik'hemik'hale kaha e ka sebetsa e le motheo o fokolang kapa asiti. Bokhoni ba eona ba ho boloka li-acid bo thusa ho boloka pH e tsitsitseng nakong ea karabelo, e leng se nolofalletsang taolo e betere le ho ikatisa hape.