4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Li-enzyme substrates: 4NPM e ka sebelisoa e le setsi sa li-enzyme tse fapaneng, ho kenyelletsa le li-glycosidase le li-enzyme tse amanang le tsona.Li-enzyme tsena li khaola maqhama a glycosidic pakeng tsa mannose le 4NPM, e leng se etsang hore ho lokolloe karolo ea nitrophenyl.Tekanyo ea substrate hydrolysis e ka lekanyetsoa spectrophotometrically ka ho beha leihlo ho monya ha sehlopha se lokolotsoeng sa nitrophenyl ka bolelele bo itseng ba maqhubu.Sena se lumella bafuputsi ho lekola tšebetso ea enzyme le kinetics.

Litlhahlobo tsa metabolism ea carbohydrate: Ka ho sebelisa 4NPM joalo ka substrate, bafuputsi ba ka ithuta tšebetso ea li-enzyme tse amehang ho metabolism ea carbohydrate, joalo ka alpha-mannosidase.Li-enzyme tsena li etsa hydrolyze litlamo tsa glycosidic ka har'a metsoako e nang le mannose, 'me mosebetsi oa bona o ka lekanyetsoa ka ho shebella ho lokolloa ha karolo ea nitrophenyl ho tloha 4NPM.

Lithuto tsa Glycosylation: 4NPM e ka boela ea sebelisoa litekong ho etsa lipatlisiso tsa li-enzyme tse amehang lits'ebetsong tsa glycosylation.Glycosylation ke mokhoa oa ho kopanya limolek'hule tsa tsoekere ho liprotheine kapa limolek'hule tse ling, 'me li-enzyme tse ngata li kenya letsoho ts'ebetsong ena.Ka ho sebelisa 4NPM joalo ka substrate e amohelang, bafuputsi ba ka lekanya phetisetso ea karolo ea tsoekere ho 4NPM ka li-enzyme tse ikhethileng tse amehang karabelong ea glycosylation.

Ho hlahloba li-enzyme inhibitors kapa activators: 4NPM e ka sebelisoa litekong tse phahameng tsa ho hlahloba ho fumana metsoako e thibelang kapa e kenyang li-enzyme tse itseng.Ka ho lekola phello ea metsoako ea liteko ho hydrolysis kapa ho fetoloa ha 4NPM ka li-enzyme tse shebiloeng, bafuputsi ba ka tseba ho tseba lisebelisoa tsa kalafo kapa li-probe tsa lik'hemik'hale tse sebetsang bakeng sa ho ithuta tšebetso ea enzyme.