4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Di-substrate tsa enzyme: 4NPM e ka sebediswa e le substrate bakeng sa di-enzyme tse fapaneng, ho kenyeletswa le di-glycosidase le di-enzyme tse amanang le tsona. Di-enzyme tsena di arola tlamo ya glycosidic pakeng tsa mannose le 4NPM, e leng se fellang ka ho lokollwa ha karolo ya nitrophenyl. Bongata ba hydrolysis ya substrate bo ka lekanyetswa ka spectrophotometric ka ho lekola ho monyela ha sehlopha sa nitrophenyl se lokollotsweng ka bolelele bo itseng ba leqhubu. Sena se dumella bafuputsi ho lekola mosebetsi wa enzyme le kinetics.

Liteko tsa metabolism ea lik'habohaedreite: Ka ho sebelisa 4NPM e le substrate, bafuputsi ba ka ithuta mosebetsi oa lienzyme tse amehang metabolism ea lik'habohaedreite, joalo ka alpha-mannosidases. Lienzyme tsena li hydrolyze li-glycosidic bond ka har'a metsoako e nang le mannose, 'me mosebetsi oa tsona o ka lekanngoa ka ho beha leihlo ho lokolloa ha nitrophenyl moiety ho tsoa ho 4NPM.

Lithuto tsa Glycosylation: 4NPM e ka boela ea sebelisoa litekong ho batlisisa lienzyme tse amehang lits'ebetsong tsa glycosylation. Glycosylation ke ts'ebetso ea ho hokela limolek'hule tsa tsoekere ho liprotheine kapa limolek'hule tse ling, 'me lienzyme tse ngata li ameha ts'ebetsong ena. Ka ho sebelisa 4NPM e le substrate ea kamohelo, bafuputsi ba ka lekanya phetisetso ea karolo ea tsoekere ho 4NPM ke lienzyme tse itseng tse amehang liketsong tsa glycosylation.

Ho hlahloba li-enzyme inhibitors kapa li-activator: 4NPM e ka sebelisoa litekong tsa tlhahlobo ea ho sebelisa matla a phahameng ho khetholla metsoako e thibelang kapa e kenyang tšebetsong lienzyme tse itseng. Ka ho hlahloba phello ea metsoako ea liteko holim'a hydrolysis kapa phetoho ea 4NPM ke lienzyme tse reriloeng, bafuputsi ba ka khetholla metsoako e ka bang teng ea phekolo kapa li-probe tsa lik'hemik'hale tse thusang bakeng sa ho ithuta mosebetsi oa lienzyme.