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1,2,3,4,6-Penta-O-acetyl-D-mannopyranose CAS:25941-03-1
1,2,3,4,6-Penta-O-acetyl-D-mannopyranose ke motsoako oa lik'hemik'hale o tsoang ho D-mannose, tsoekere e bonolo.Ke motsoako moo lihlopha tsa acetyl li hokeloang ho lihlopha tse hlano ho tse tšeletseng tsa hydroxyl tse teng ka har'a molek'hule ea mannose.Mofuta ona oa acetylated oa D-mannose o atisa ho sebelisoa ho organic synthesis le lipatlisiso tsa lik'hemik'hale e le mohaho oa mohaho kapa thepa ea ho qala bakeng sa motsoako oa limolek'hule tse rarahaneng.Lihlopha tsa acetyl li fana ka botsitso 'me li ka fetola reactivity le thepa ea motsoako.
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1,2,3,4-Di-O-Isopropylidene-alpha-D-galactopyranose CAS:4064-06-6
1,2:3,4-Di-O-isopropylidene-D-galactopyranose ke motsoako oa lik'hemik'hale oa lelapa la galactopyranose derivatives.E sebelisoa hangata ho k'hemistri ea lintho tse phelang e le ntho e sireletsang lihlopha tsa hydroxyl tse teng ka tsoekere, haholo-holo galactose.Motsoako ona o entsoe ka ho sebetsana le D-galactose le acetone ho etsa derivative ea diacetone, e ntan'o phekoloa ka asiti ho etsa motsoako oa di-O-isopropylidene.Motsoako ona o sireletsa lihlopha tsa hydroxyl, ho thibela liketso tse sa batleheng nakong ea motsoako oa lik'hemik'hale, 'me o ka tlosoa ka mokhoa o khethiloeng ho tsosolosa motsoako oa pele.Sebopeho sa eona se kopaneng le botsitso li etsa hore e be molemo lits'ebetsong tse fapaneng tšimong ea organic synthesis.
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X-GAL CAS: 7240-90-6 Theko ea Moetsi
5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) ke substrate e tloaelehileng ea chromogenic e sebelisoang ho baeloji ea limolek'hule le lits'ebetsong tsa biochemistry.E sebelisoa haholo bakeng sa ho lemoha mofuta oa lacZ, o kenyelletsang enzyme β-galactosidase.
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PNPG CAS: 3150-24-1 Theko ea Moetsi
PNPG, kapa p-nitrophenyl β-D-glucopyranoside, ke karoloana e nyenyane ea molek'hule e atisang ho sebelisoa litlhahlobong tsa lik'hemik'hale tsa biochemical ho lekanya tšebetso ea li-enzyme tsa glucosidase.Ha e na 'mala ebile ha e na fluorescent, empa ha hydrolysis e etsoa ke glucosidase, e fetoloa ho p-nitrophenol, e nang le' mala o mosehla 'me e bonahala habonolo spectrophotometrically.
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ONPG CAS: 369-07-3 Theko ea Moetsi
O-nitrophenyl-β-D-galactopyranoside (ONPG) ke substrate ea maiketsetso e sebelisoang litlhahlobong tsa biochemical le molecular biology ho lekanya tšebetso ea enzyme β-galactosidase.Hangata e sebelisoa litlhahlobong tsa ho lemoha liphatsa tsa lefutso tsamaisong ea baktheria, joalo ka Escherichia coli.ONPG ke motsoako o se nang 'mala o phunyeletsoeng ke β-galactosidase, e leng se hlahisang ho lokolloa ha motsoako o mosehla, o-nitrophenol.Mmala o mosehla o hlahisoang o ka lekanyetsoa spectrophotometrically, ho fana ka tekanyo e sa tobang ea ts'ebetso ea enzyme.Teko e sebelisang ONPG hangata e bitsoa tlhahlobo ea ONPG mme ke mokhoa o sebelisoang haholo lipatlisisong tsa baeloji ea limolek'hule ho lekola maemo a polelo a liphatsa tsa lefutso tse laoloang ke lac. operon liseleng tsa baktheria.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) ke sesupo sa redox se atisang ho sebelisoa litlhahlobong tsa baeloji le tsa biochemical.Ke phofo e mosehla e mosehla e fetohang boputsoa ha e fokotsehile, e etsa hore e be molemo bakeng sa ho lemoha boteng ba li-enzyme tse itseng le ts'ebetso ea metabolism.
NBT e sebetsana le li-electron carriers le li-enzyme tse kang dehydrogenases, tse amehang mekhoeng e sa tšoaneng ea lisele.Ha NBT e fokotsoa ke li-enzyme tsena, e etsa putsoa ea formazan precipitate, e lumellang ho lemoha pono kapa spectrophotometric.
Reagent ena e atisa ho sebelisoa litlhahlobong tse kang tlhahlobo ea phokotso ea NBT, moo e sebelisetsoang ho lekola tšebetso ea phefumoloho ea lisele tsa 'mele.E ka boela ea sebelisoa ho ithuta mesebetsi ea enzyme le litsela tsa metabolic lipatlisisong tse amanang le khatello ea oxidative, bokhoni ba lisele, le phapang ea lisele.
NBT e fumane lits'ebetso mafapheng a fapaneng, ho kenyeletsoa microbiology, immunology, le cell biology.E na le mekhoa e mengata, e tsitsitse, 'me e bonolo ho e sebelisa, e etsa hore e be khetho e tsebahalang bakeng sa liprothokholo tse ngata tsa liteko.
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Neocuproine CAS: 484-11-7 Theko ea Moetsi
Neocuproine ke chelating agent e sebelisoang hangata lits'ebetsong tse fapaneng, ho kenyeletsoa chemistry ea tlhahlobo le meriana.E na le kamano e matla bakeng sa li-ion tsa koporo 'me e etsa li-complexes tse tsitsitseng le tsona.Thepa ena e etsa hore neocuproine e be molemo bakeng sa ho bona le ho lekanya koporo ka tharollo kapa lisampole.Ho feta moo, neocuproine e ithutoe bakeng sa ts'ebeliso ea eona ea kalafo e ka bang teng, haholoholo kalafong ea mofetše le mafu a neurodegenerative.
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IPTG CAS: 367-93-1 Theko ea Moetsi
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ke analoge ea maiketsetso ea lactose e sebelisoang hangata liphuputsong tsa baeloji ea limolek'hule le lits'ebetsong tsa biotechnology.IPTG e sebelisoa haholo-holo ho susumetsa tlhahiso ea liphatsa tsa lefutso tsamaisong ea baktheria, moo e sebetsang e le sesosa sa molek'hule ho qala ho ngoloa ha liphatsa tsa lefutso.
Ha e kenyelelitsoe mocheng oa ho hōla, IPTG e nkoa ke libaktheria 'me e ka tlama protheine ea lac repressor, e thibela ho thibela mosebetsi oa lac operon.Lac operon ke sehlopha sa liphatsa tsa lefutso tse amehang ho metabolism ea lactose, 'me ha protheine e hatellang e tlosoa, liphatsa tsa lefutso li hlahisoa.
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HATU CAS:148893-10-1 Theko ea Moetsi
HATU (1- [bis(dimethylamino) methylene] -1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) ke reagent e atisang ho sebelisoa ho kopanya peptide synthesis le organic chemistry.
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D-fucose CAS: 3615-37-0 Theko ea Moetsi
D-fucose ke monosaccharide, haholo-holo tsoekere e nang le carbon e tšeletseng, e leng ea sehlopha sa tsoekere e bonolo e bitsoang hexoses.Ke isomer ea tsoekere, e fapaneng ka tlhophiso ea sehlopha se le seng sa hydroxyl.
D-fucose ka tlhaho e fumaneha linthong tse fapaneng, ho kenyelletsa libaktheria, li-fungus, limela le liphoofolo.E bapala karolo ea bohlokoa lits'ebetsong tse 'maloa tsa tlhaho, joalo ka ho bonts'a lisele, ho khomarela lisele, le glycoprotein synthesis.Ke karolo ea glycolipids, glycoprotein, le proteoglycans, tse amehang puisanong ea sele le lisele le kananelo.
Ho batho, D-fucose e boetse e ameha ho biosynthesis ea likarolo tsa bohlokoa tsa glycan, tse kang li-antigen tsa Lewis le li-antigens tsa sehlopha sa mali, tse nang le liphello tsa ho lumellana le tšelo ea mali le ho ba le tšoaetso ea mafu.
D-fucose e ka fumanoa mehloling e fapaneng, ho kenyelletsa le limela tsa leoatleng, limela le ho belisoa ha likokoana-hloko.E sebelisoa lipatlisisong le lits'ebetsong tsa biomedical, hammoho le tlhahiso ea meriana e itseng le metsoako ea phekolo.
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DDT CAS: 3483-12-3 Theko ea Moetsi
DL-Dithiothreitol, eo hape e tsejoang e le DTT, ke ntho e fokotsang e sebelisoang haholo lipatlisisong tsa biochemical le molecular biology.Ke molek'hule e nyane e nang le sehlopha sa thiol (se nang le sebabole) pheletsong ka 'ngoe.
Hangata DTT e sebelisoa ho khaola maqhama a disulfide ka liprotheine, tse thusang ho li hlahisa kapa ho li hlahisa.Phokotso ena ea litlamo tsa disulfide e bohlokoa lits'ebetsong tse fapaneng tsa laboratori tse kang tlhoekiso ea protheine, gel electrophoresis, le lithuto tsa sebopeho sa protheine.DTT e ka boela ea sebelisoa ho sireletsa lihlopha tsa thiol le ho thibela oxidation nakong ea mekhoa ea liteko.
DTT ka tloaelo e eketsoa ho tharollo ea liteko maemong a manyane, mme ts'ebetso ea eona e ipapisitse le boteng ba oksijene.Ke habohlokoa ho sebetsana le DTT ka hloko kaha e na le maikutlo a moea, mocheso le mongobo, e leng se ka fokotsang katleho ea eona.
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D-(+)-Galactose CAS: 59-23-4 Theko ea Moetsi
D-(+) -Galactose ke tsoekere ea monosaccharide le karolo ea bohlokoa ea mekhoa e mengata ea likokoana-hloko.Ke tsoekere ea tlhaho e fumanehang lijong tse ngata, joalo ka litholoana, lihlahisoa tsa lebese le meroho.
Galactose e atisa ho sebelisoa 'meleng ka letoto la karabelo ea enzymatic.E bapala karolo ea bohlokoa puisanong ea lisele, tlhahiso ea matla, le biosynthesis ea limolek'hule tsa bohlokoa joalo ka glycolipids, glycoprotein le lactose.
Mabapi le lits'ebetso tsa eona, D-(+) -Galactose e sebelisoa hangata ho microbiology le biotechnology e le mohloli oa khabone mecheng ea litaba tsa setso bakeng sa kholo ea likokoana-hloko tse fapaneng.E boetse e sebelisoa ho hlahisa metsoako e fapaneng ea bioactive, meriana le lihlahisoa tsa lijo.Ho feta moo, hangata e sebelisoa e le moemeli oa tlhahlobo ea bongaka, haholo litekong tsa ho lekola tšebetso ea sebete le ho lemoha mathata a lefutso a amanang le metabolism ea galactose.
