Nitrotetrazolium Blue Chloride CAS:298-83-9

Nitrotetrazolium Blue Chloride (NBT) ke sesupo sa redox se sebediswang haholo ditekong tsa baeloji le tsa biochemical. Ke phofo e mosehla o mosehla e fetohang boputswa ha e fokotswa, e leng se etsang hore e be molemo bakeng sa ho lemoha boteng ba dienzyme tse itseng le tshebetso ya metabolism.

Phello e ka sehloohong ea NBT ke ho thehoa ha formazan precipitate e putsoa ha e fokotsoa ke lienzyme tse itseng. Phetoho ena ea 'mala e lumella ho lemoha mosebetsi oa lienzyme ka pono kapa ka spectrophotometric.

NBT e na le mefuta e fapaneng ea lits'ebeliso lipatlisisong le tlhahlobong ea mafu. Tse ling tsa ts'ebeliso ea eona ea mantlha ke tsena:

Liteko tsa ts'ebetso ea enzyme: NBT e ka sebelisoa ho lekanya ts'ebetso ea li-dehydrogenase, tse amehang lits'ebetsong tse kang ho hema ha sele le metabolism. Ka ho beha leihlo phokotso ea NBT ho formazan, bafuputsi ba ka hlahloba ts'ebetso ea li-enzyme tsena.

Tlhahlobo ea ts'ebetso ea sele ea 'mele ea ho itšireletsa mafung: NBT e sebelisoa hangata tekong ea phokotso ea NBT ho lekola ts'ebetso ea ho phatloha ha ho hema ha lisele tsa 'mele ea ho itšireletsa mafung, haholo-holo li-phagocyte. Teko ena e lekanya bokhoni ba lisele tsena ba ho hlahisa mefuta ea oksijene e arabelang, e ka fokotsang NBT le ho theha ho putlama ho putlama.

Patlisiso ea microbiology: NBT e sebelisoa ho microbiology ho ithuta metabolism ea microbial le ho lekola ts'ebetso ea lienzyme tse itseng. Mohlala, e sebelisitsoe ho lemoha li-reductase tsa baktheria tsa nitrate kapa libaktheria tse etsang formazan.

Lithuto tsa ho phela ha sele: Phokotso ea NBT e lumella bafuputsi ho lekola ts'ebetso ea metabolism le ho phela ha lisele. Ka ho lekanya matla a sehlahisoa sa blue formazan, hoa khoneha ho fumana palo ea lisele tse phelang sampole e fanoeng.