ONPG CAS:369-07-3 Theko ea moetsi

Tšusumetso ea ONPG e le substrate e tla khaoloa ke enzyme β-galactosidase, e leng se fellang ka ho lokolloa ha sehlahisoa se setala, o-nitrophenol. Phetoho ena ea 'mala e ka lekanngoa ka spectrophotometric, e lumellang ho lekanngoa ha mosebetsi oa β-galactosidase. Tšebeliso ea ONPG e haholo-holo tlhahlobong ea tlhahiso ea liphatsa tsa lefutso lipatlisisong tsa baeloji ea limolek'hule le microbiology. Hangata e sebelisoa ho lekanya mosebetsi oa β-galactosidase e le moqolotsi oa litaba bakeng sa lithuto tsa tlhahiso ea liphatsa tsa lefutso, haholo-holo libaktheria tse kang E. coli. Jini ea lacZ, e kenyang β-galactosidase, hangata e sebelisoa e le letšoao la tlhahlobo ea tlhahiso ea liphatsa tsa lefutso, kaha tlhahiso ea eona e ka susumetsoa ke maemo a itseng kapa ea laoloa ke batšehetsi ba itseng. Teko ea ONPG e fana ka mokhoa o bonolo le o tšepahalang oa ho lekola boemo ba tlhahiso ea liphatsa tsa lefutso ka ho lekanya mosebetsi oa β-galactosidase. Teko ena e sebelisoa haholo lits'ebetsong tse fapaneng, joalo ka ho ithuta mosebetsi oa batšehetsi, taolo ea liphatsa tsa lefutso, le likamano tsa protheine le protheine. Ho phaella moo, e ka sebediswa ho fumana kinetics ya enzyme le ho lekola ditlamorao tsa diphetoho kapa kalafo hodima tshebetso ya enzyme.