X-GAL CAS: 7240-90-6 Theko ea moetsi

Phetoho ea 'Mala: Hangata X-Gal ha e na 'mala empa, ha e haelloa ke hydrolysis ka β-galactosidase, e fetoha e putsoa. ​​Phetoho ena ea 'mala e lumella ho lemoha ka pono le ho lekanya mosebetsi oa β-galactosidase.

Ho Fumana Lefutso la LacZ: X-Gal e sebelisetsoa ho khetholla lisele kapa metsoako ea liphatsa tsa lefutso e hlahisang jini ea lacZ. LacZ e atisa ho sebelisoa e le jini ea moqolotsi oa litaba baelojing ea limolek'hule ho lekola tlhahiso ea jini kapa mosebetsi oa ho khothaletsa thuto.

Tlhahlobo ea Kolone: ​​X-Gal e atisa ho sebelisoa litekong tsa tlhahlobo ea likolone tsa baktheria. Likolone tsa baktheria tse hlahisang LacZ li bonahala li le putsoa ha li holisoa holim'a agar e nang le X-Gal, e leng se nolofalletsang ho khetholla le ho khetha likolone tse nang le lacZ habonolo.

Tlhahlobo ea ho Kopanya Liphatsa tsa Lefutso: X-Gal e boetse e sebelisoa litekong tsa ho kopanya liphatsa tsa lefutso. Ha liphatsa tsa lefutso tse reretsoeng li hokahantsoe le liphatsa tsa lefutso tsa lacZ, ho taka ha X-Gal ho ka senola mokhoa oa tlhahiso ea protheine ea ho kopanya ka har'a sele kapa lisele.

Ho ba Sebakeng sa Protheine: Ho daea X-Gal ho ka sebelisoa ho batlisisa ho ba sebakeng sa protheine e ka tlas'a sele. Ka ho kopanya protheine e khahlisang le gene ea lacZ, ts'ebetso ea β-galactosidase e ka bontša moo protheine e fumanehang teng ka har'a sele.

Li-analogue tsa X-Gal: Mefuta e fetotsoeng ea X-Gal, joalo ka Bluo-Gal kapa Red-Gal, e ntlafalitsoe ho lumella merero e meng ea nts'etsopele ea mebala. Li-analogue tsena li nolofalletsa phapang lipakeng tsa lisele kapa lisele tse nang le lacZ-positive le lacZ-negative ho sebelisoa mebala e fapaneng.