Phenylgalactoside CAS: 2818-58-8

Phello mosebetsing oa enzyme: Phenylgalactoside e sebelisoa hangata ho lekanya tšebetso ea enzyme β-galactosidase.Ha phenylgalactoside e entsoe ka hydrolyzed ke β-galactosidase, e ntša p-nitrophenol.Ho bokellana ha p-nitrophenol ho ka lekanyetsoa ka bongata, ho fana ka leseli mabapi le tšebetso ea β-galactosidase.Phello ena e sebelisoa lits'ebetsong tse joalo ka liteko tsa enzyme le litsamaiso tsa tlhahlobo.

Tlhahlobo ea polelo ea liphatsa tsa lefutso: Phenylgalactoside hangata e sebelisoa e le setsi sa liteko tsa baeloji ea limolek'hule ho ithuta polelo ea lefutso.Mofuta oa lacZ, o kenyelletsang β-galactosidase, hangata o kopanngoa le tatelano ea taolo ea mefuta e meng ea thahasello.Polelo ea lefutso la lacZ le hydrolysis ea phenylgalactoside ka β-galactosidase e ka bonts'a mokhoa oa polelo le boemo ba mofuta o ithutoang.

Sistimi ea ho hlahloba: Phenylgalactoside ke karolo ea bohlokoa ea lits'ebetso tsa tlhahlobo tse sebelisang ts'ebetso ea β-galactosidase.Mohlala o mong o tsebahalang haholo ke mokhoa oa ho hlahloba bosoeu bo boputsoa, ​​​​o sebelisoang ho hloaea lisele tse kopaneng kapa tse fetotsoeng litekong tsa baeloji ea limolek'hule.Likolone tse atlehileng ho nka DNA e ncha kapa tse entsoeng bocha li tla hlahisa β-galactosidase, e lebisang ho hydrolysis ea phenylgalactoside le ho thehoa ha 'mala o moputsoa.

Ho hloekisa protheine: Maemong a mang, phenylgalactoside e ka sebelisoa e le ligand bakeng sa affinity chromatography ho hloekisa liprotheine tse tlamang ka ho khetheha kapa tse sebelisoang ke β-galactosidase.Protheine e khahlang e kanna ea ba le tag ea affinity kapa tag ea fusion e nang le β-galactosidase-binding domain.Ka ho fetisa motsoako oa protheine ka har'a kholomo e nang le immobilized phenylgalactoside, protheine e lakatsehang e ka bolokoa ka mokhoa o ikhethileng 'me hamorao ea hlakoloa.