Liphaephe CAS: 5625-37-6 Theko ea moetsi

LIPHAEPE (piperazine-1,4-bisethanesulfonic acid) ke motsoako oa zwitterionic buffering o sebelisoang haholo-holo lipatlisisong tsa baeloji le tsa biochemical. E na le likarolo le lits'ebetso tse 'maloa tsa bohlokoa, ho kenyeletsoa:

Moemeli oa ho boloka pH: LI-PIPES ke sesebelisoa se sebetsang hantle se thusang ho boloka mefuta e tsitsitseng ea pH litekong tse fapaneng tsa baeloji. E sebelisoa hangata mecheng ea temo ea sele, litekong tsa enzyme, le lits'ebetsong tsa baeloji ea limolek'hule.

Bokgoni bo phahameng ba ho thibela: LI-PIPE li na le bokhoni bo botle ba ho thibela ka har'a pH e pakeng tsa 6.1 le 7.5, e leng se etsang hore e lokele ho boloka maemo a tsitsitseng a pH lits'ebetsong tse fapaneng tsa baeloji.

Tšebelisano e fokolang le limolek'hule tsa biomolecules: LI-PIPES li tsebahala ka tšitiso e fokolang ea tsona lits'ebetsong tsa biochemical le ho tlamahana ho fokolang le liprotheine le li-enzymes, e leng se etsang hore e be e loketseng ho boloka botšepehi le ts'ebetso ea limolek'hule tsa biomolecules.

E loketse liteko tse itšetlehileng ka mocheso: LI-PIPES li khona ho boloka thepa ea tsona ea ho thibela mocheso maemong a fapaneng a mocheso, ho kenyeletsoa le mocheso oa 'mele le o phahameng. Sena se etsa hore e lokele liteko tse hlokang botsitso le ho nepahala maemong a fapaneng a mocheso.

Ditshebediso tsa Electrophoresis: Di-PIPE di sebediswa hangata e le sethibela-mafu mekgweng ya gel electrophoresis, jwalo ka RNA kapa DNA agarose gel electrophoresis, ka lebaka la ho monya ha yona mahlaseli a kotsi a UV a tlase le bokgoni bo phahameng ba ho tsamaisa motlakase.

Tlhahiso ea meriana: LI-PIPES li boetse li sebelisoa tlhahisong ea meriana e le sesebelisoa sa ho thibela, ho fana ka botsitso le ho boloka pH e ntle ka ho fetisisa bakeng sa katleho ea meriana.