NSP-SA-NHS CAS: 199293-83-9 Theko ea Moetsi

NSP-SA-NHS, eo hape e tsejoang e le N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, ke motsoako o atisang ho sebelisoa e le thiol-specific crosslinking reagent ka bioconjugation reactions.Phello ea eona e kholo ke ho thehoa ha maqhama a tsitsitseng a thioester pakeng tsa lihlopha tsa thiol tse teng ho li-biomolecules, tse kang liprotheine kapa li-peptide.

Tšebeliso ea NSP-SA-NHS e haholo-holo tšimong ea phetoho ea liprotheine le immobilization.Tse ling tsa lisebelisoa tsa eona tsa bohlokoa li kenyelletsa:

Protein labeling: NSP-SA-NHS e sebelisetsoa ho hokela lileibole ka kopanelo, joalo ka lidae tsa fluorescent kapa biotin, ho liprotheine kapa li-peptide.Sena se lumella ho lemoha, ho tsebahatsa le ho lateloa ha li-biomolecules tse ngotsoeng litekong tse fapaneng tsa baeloji.

Tšebelisano ea protheine: NSP-SA-NHS e ka sebelisoa ho tšela liprotheine tse sebelisanang le liprotheine ho ithuta ho sebelisana ha liprotheine.Hangata sena se sebelisoa mekhoeng e kang co-immunoprecipitation kapa litlhahlobo tsa ho theola boima ba 'mele ho khetholla balekane ba tlamang kapa liprotheine tsa boithuto.

Protein immobilization: NSP-SA-NHS e lumella ho hokelloa ha liprotheine kapa li-peptide holim'a libaka tse tiileng, ho kenyeletsoa lifaha tsa agarose, lifaha tsa makenete, kapa li-microplates.Sena se bohlokoa lits'ebetsong tse joalo ka tlhoekiso ea kamano, tlhahlobo ea lithethefatsi, kapa nts'etsopele ea biosensor.

Phetoho ea bokaholimo: NSP-SA-NHS e ka sebelisoa ho fetola bokaholimo, joalo ka li-slide tsa khalase kapa li-nanoparticles, tse nang le liprotheine kapa li-peptide, ho theha libaka tse sebetsang ka biomolecule bakeng sa lits'ebetso tse fapaneng joalo ka tlhahlobo, lits'ebetso tsa phano ea lithethefatsi, kapa sethala sa bio-sensing.