3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ho lemoha ts'ebetso ea beta-galactosidase: ONPG hangata e sebelisoa ho fumana boteng le ts'ebetso ea beta-galactosidase lisampoleng tse fapaneng tsa baeloji, joalo ka litso tsa baktheria kapa li-lysate tsa sele. Tlhahiso ea o-nitrophenol, e nang le 'mala o mosehla, e ka lekanngoa habonolo ho sebelisoa spectrophotometer.

Lithuto tsa tlhahiso ea liphatsa tsa lefutso: ONPG e sebelisoa hangata lipatlisisong tsa baeloji ea limolek'hule ho ithuta tlhahiso ea liphatsa tsa lefutso. Ka ho kopanya mothetsi oa liphatsa tsa lefutso tse khahlisang le liphatsa tsa lefutso tse kenyang beta-galactosidase, bafuputsi ba ka lekanya ts'ebetso ea mothetsi enoa ka ho eketsa ONPG le ho lekanya tlhahiso ea o-nitrophenol e hlahang. Mokhoa ona, o tsejoang e le teko ea motlalehi oa beta-galactosidase, o fana ka tlhahisoleseling mabapi le ts'ebetso ea ho ngola ea liphatsa tsa lefutso.

Tlhahlobo ea baktheria: Libaktheria tse ling li hlahisa beta-galactosidase, ha tse ling li sa e hlahise. ONPG e ka sebelisoa hammoho le liteko tse ling tsa biochemical ho khetholla mefuta ea baktheria ho latela bokhoni ba eona ba ho hydrolyze ONPG. Mokhoa ona o sebelisoa hangata lilaboratoring tsa tlhahlobo ea bongaka le tsa microbiology.

Ho hlahloba dithibela-enzyme kapa di-activator: ONPG e ka sebediswa ho hlahloba dikhemikhale tse fetolang tshebetso ya beta-galactosidase. Ka ho lekanya tshebetso ya dikhemikhale moo ho nang le dikhemikhale tse fapaneng, bafuputsi ba ka hlwaya dithibela-enzyme kapa di-activator tse ka fuputswang hape bakeng sa bokgoni ba tsona ba kalafo.