HEPBS CAS:161308-36-7 Theko ea moetsi

N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) (HEPBS) ke sesebelisoa sa zwitterionic se sebelisoang haholo lipatlisisong tsa baeloji le tsa biochemical. Phello ea sona ea mantlha ke ho thusa ho boloka pH e tsitsitseng litharollong, haholo-holo ka har'a mefuta ea pH ea fisioloji (7.2-7.4).

Tšebeliso e ka sehloohong eaHEPBS e teng ka hara setso sa sele, moo e sebediswang e le karolo ya tlwaelo ya temo ho boloka pH ya tharollo. E thusa ho fana ka tikoloho e tsitsitseng bakeng sa kgolo ya sele le ho thibela diphetoho dife kapa dife tse ka bang teng tsa pH tse ka bang kotsi ho disele.

HEPBS e boetse e sebelisoa e le sesebelisoa sa ho thibela li-enzyme lithutong tsa enzyme, kaha e ka tsitsisa pH nakong ea liketso tsa enzyme. E sebelisoa hangata tlhoekisong ea protheine le litekong tsa enzyme ho ntlafatsa ts'ebetso le botsitso ba li-enzyme.

Ho feta moo,HEPBS e sebelisoa mekhoeng e fapaneng ea electrophoretic, joalo ka gel electrophoresis le capillary electrophoresis, ho boloka pH e lakatsehang le ho tsitsisa limolek'hule tse tjhajisitsoeng tse arohantsoeng.

Ntle le thepa ea eona ea buffer,HEPBS e ka boela ea sebetsa e le thibelo e fokolang ea liprotheine tse itseng tsa tšepe le li-enzyme, e leng se etsang hore e be molemo lits'ebetsong tse itseng.