HEPES-Na CAS: 75277-39-3 Theko ea Moetsi

Buffering agent: HEPES letsoai la sodium le sebelisoa hangata joalo ka ntho e sireletsang litekong tsa baeloji le tsa biochemical.E thusa ho boloka pH e tsitsitseng, haholo-holo ka har'a physiological range (pH 7.2-7.6).Bokhoni ba eona ba ho thibela bo etsa hore e be ea bohlokoa ho boloka maemo a nepahetseng bakeng sa karabelo e fapaneng ea enzymatic, setso sa lisele le mekhoa ea baeloji ea limolek'hule.

Setso sa lisele: Letsoai la HEPES la sodium le sebelisoa haholo joalo ka ntho e sireletsang mecheng ea litaba tsa setso sa lisele.Bokhoni ba ho boloka pH e tsitsitseng bo bohlokoa bakeng sa kholo e nepahetseng le ho sebetsa ha lisele.HEPES hangata e ratoa ho feta lisebelisoa tse ling tse thibelang likokoana-hloko kaha ha e bontše liphetoho tse kholo pH ha e pepesehetse CO2 ea sepakapaka.

Lithuto tsa enzyme: letsoai la sodium la HEPES le thusa haholo lithutong tsa enzymatic moo tikoloho ea pH e sa fetoheng le e laoloang e hlokahalang.E thibela ho feto-fetoha ho hoholo ha pH nakong ea karabelo ea enzymatic, e netefatsa ts'ebetso e nepahetseng ea enzyme.

Electrophoresis: HEPES letsoai la sodium le atisa ho sebelisoa e le ntho e thibelang likokoana-hloko mekhoeng e fapaneng ea electrophoretic e kang polyacrylamide gel electrophoresis (PAGE) le agarose gel electrophoresis.E thusa ho boloka pH ea buffer, e leng bohlokoa bakeng sa ho arola le ho sekaseka nucleic acid le liprotheine.

Litlhahlobo tsa lik'hemik'hale: letsoai la sodium la HEPES le sebelisoa hangata litekong tse fapaneng tsa lik'hemik'hale tsa biochemical, ho kenyelletsa le li-enzyme, li-protein quantification assays, le li-spectrophotometric.E thusa ho boloka pH e batloang e hlokahalang bakeng sa liphetho tse nepahetseng le tse ka hlahisoang hape.

Tlhahiso ea lithethefatsi: letsoai la sodium la HEPES le boetse le sebelisoa mecheng ea meriana e le ntho e thibelang ho tsitsisa metsoako ea lithethefatsi le ho boloka pH e lakatsehang.