HEPES-Na CAS:75277-39-3 Theko ea moetsi

Moemeli oa ho buffer: Letsoai la sodium la HEPES le sebelisoa hangata e le moemeli oa ho buffer litekong tsa baeloji le tsa biochemical. E thusa ho boloka mefuta e tsitsitseng ea pH, haholo-holo mefuta ea 'mele (pH 7.2-7.6). Bokhoni ba eona ba ho buffer bo e etsa hore e be ea bohlokoa ho bolokeng maemo a nepahetseng bakeng sa liketso tse fapaneng tsa enzymatic, setso sa sele le mekhoa ea baeloji ea limolek'hule.

Temo ea lisele: Letsoai la sodium la HEPES le sebelisoa haholo e le sesebelisoa sa ho thibela lisele tse holang. Bokhoni ba ho boloka pH e tsitsitseng bo bohlokoa bakeng sa kholo e nepahetseng le ho phela hantle ha lisele. HEPES hangata e ratoa ho feta lisebelisoa tse ling tsa ho thibela lisele tse phelang kaha ha e bontše liphetoho tse kholo pH ha e pepesehetse CO2 ea sepakapaka.

Lithuto tsa Enzyme: Letswai la sodium la HEPES le thusa haholo lithutong tsa enzymatic moo ho hlokahalang tikoloho ea pH e sa fetoheng le e laoloang. E thibela ho feto-fetoha ho hoholo ha pH nakong ea liketso tsa enzymatic, e leng se netefatsang ts'ebetso e ntle ea enzyme.

Electrophoresis: Letswai la sodium la HEPES le atisa ho sebediswa e le sesebelisoa sa ho thibela dikokwana-hloko mekgweng e fapaneng ya electrophoresis jwalo ka polyacrylamide gel electrophoresis (PAGE) le agarose gel electrophoresis. E thusa ho boloka pH ya buffer, e leng ya bohlokwa bakeng sa ho arola le ho sekaseka di-nucleic acid le diprotheine.

Liteko tsa Biochemical: Letswai la sodium la HEPES le atisa ho sebelisoa litekong tse fapaneng tsa biochemical, ho kenyeletsoa liteko tsa enzyme, liteko tsa protein quantification, le liteko tsa spectrophotometric. E thusa ho boloka mefuta e lakatsehang ea pH e hlokahalang bakeng sa liphetho tse nepahetseng le tse ka ikatisang hape.

Tlhahiso ea meriana: Letsoai la sodium la HEPES le boetse le sebelisoa mefuteng ea meriana e le sesebelisoa sa ho thibela ho tsitsisa metsoako ea meriana le ho boloka pH e lakatsehang.