N-Ethylmaleimide CAS:128-53-0 Theko ea moetsi

N-Ethylmaleimide (NEM) ke motsoako o monyane wa tlhaho o sebediswang haholo dipatlisisong tsa biochemistry le baeloji ya dimolek'hule. E sebetsa e le thibelo e ikgethang ya dihlopha tsa protheine sulfhydryl (thiol) ka ho fetola le ho thibela tshebetso ya tsona ka tsela e ke keng ya fetolwa. NEM e arabela haholo dihlopheng tsa sulfhydryl, tse kang tse fumanwang ho amino acid cysteine, mme e ka arabela ka dihlopha tsa free sulfhydryl le tse ka hara diprotheine. Sena se etsa hore NEM e be sesebediswa se molemo bakeng sa ho ithuta tshebetso ya protheine, dikamano tsa protheine le protheine, le mosebetsi wa enzyme. Dithoto tsa yona tsa thibelo di sebedisitswe dikopong tse fapaneng, ho kenyeletswa proteomics, enzymology, biology ya sebopeho, le ho sibolla dithethefatsi.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Phetoho ea Protheine: NEM e fetola masala a cysteine ka mokhoa o ke keng oa fetoloa liprotheineng ka ho etsa motsoako o kopantsoeng. Phetoho ena e ka ama ts'ebetso ea protheine, sebopeho le likamano. Ho arabela ha NEM ka lihlopha tsa sulfhydryl ho e etsa sesebelisoa sa bohlokoa sa ho ithuta ts'ebetso ea protheine le lits'ebetso tse itšetlehileng ka thiol.

Thibelo ea Li-enzyme: Li-enzyme tse ngata li itšetlehile ka lihlopha tsa thiol bakeng sa ts'ebetso ea tsona ea ho susumetsa. Ka ho arabela le lihlopha tsa thiol, NEM e ka thibela li-enzyme tse itseng ka mokhoa o ikhethileng. Sena se 'nile sa sebelisoa haholo ho ithuta kinetics ea li-enzyme, ho utloisisa mekhoa ea li-enzyme, le ho hlahloba li-enzyme tse ka bang teng.

Lithuto tsa Tšebelisano ea Protheine le Protheine: Hangata NEM e sebelisoa ho batlisisa likamano tsa protheine le protheine. Ka ho thibela masala a cysteine a arabelang ka khetho, NEM e thibela ho thehoa ha maqhama a disulfide pakeng tsa liprotheine tse sebelisanang, ka hona e sitisa tšebelisano ea tsona. Sena se lumella bafuputsi ho ithuta karolo ea li-cysteine tse itseng likamanong tsa protheine le protheine.

Lithuto tsa Taolo ea Redox: NEM e sebelisoa ho ithuteng mekhoa ea taolo ea redox liprotheineng. Ka ho fetola li-cysteine tse arabelang, NEM e ka tsitsisa libopeho tse itseng tsa protheine le ho thibela oxidation e sa batleheng kapa phokotso ea masala a cysteine. Sena se thusa ho utloisisa karolo ea lipontšo tsa redox le litsela tsa taolo tse utloang redox.

Lithuto tsa ho Kopanya: NEM e ka sebelisoa e le moemeli oa ho hokahanya ho tsitsisa metsoako ea protheine kapa likamano tsa protheine le protheine. Ka ho fetola masala a cysteine, NEM e ka theha maqhama a kopaneng pakeng tsa liprotheine tse sebelisanang, e leng se lumellang bafuputsi ho batlisisa metsoako ea protheine le meaho ea tsona. Mokhoa ona o thusa haholo lithutong tsa baeloji ea sebopeho le tsa proteomics.

Ho Sibolloa ha Meriana: Ka lebaka la bokhoni ba eona ba ho sebelisana ka mokhoa o ikhethileng le masala a cysteine, NEM e 'nile ea sebelisoa lipatlisisong tsa ho sibolloa ha meriana. E ka sebelisoa ho khetholla le ho hlalosa lipheo tse ka bang teng tsa meriana kapa ho ithuta ho hlaka ha meriana e ka khethoang ho liprotheine tse nang le cysteine.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C6H7NO2
Teko	99%
Ponahalo	phofo e tšoeu ea kristale
Nomoro ea CAS	128-53-0
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.