4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Tekolo ea tšebetso ea enzyme: Hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside ka li-enzyme tse kang β-glucosidase e ka sebelisoa ho lekanya tšebetso ea enzyme.Ho lokolloa ha 4-nitrophenol ho ka baloa ka ho sebelisa spectrophotometry, ho lumella ho etsa qeto ea enzyme kinetics le lithuto tsa thibelo.

Tlhaloso ea liphatsa tsa lefutso le litlhahlobo tsa moqolotsi oa litaba: 4-Nitrophenyl-beta-D-glucopyranoside e ka kopanngoa le li-tag tse khethehileng tsa molek'hule tse kenyelletsoeng liprotheine tsa fusion.Ts'ebetso ea enzyme ea protheine ea fusion e ka lekanyetsoa habonolo ka ho lekanya ho lokolloa ha 4-nitrophenol, ho fana ka leseli mabapi le polelo ea liphatsa tsa lefutso le likamano tsa protheine le protheine.

Ho sibolloa ha lithethefatsi le tlhahlobo e phahameng ea lihlahisoa: Hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside e ka sebelisoa e le tlhahlobo ea ts'ebetso ea ho sibolla lithethefatsi.E lumella ho hlahlojoa ka potlako ha lilaebrari tse kholo tsa metsoako ho tseba li-inhibitors kapa li-activator tsa enzyme.

Teko ea ho hlahloba: Mafu a mang a amahanngoa le liphetoho mesebetsing e itseng ea enzyme.Ka ho sebelisa hydrolysis ea 4-Nitrophenyl-beta-D-glucopyranoside ka li-enzyme tse khethehileng e le tlhahlobo ea tlhahlobo, mafu ana a ka fumanoa kapa a hlahlojoa.Sena se kenyelletsa maemo a amanang le mathata a polokelo ea lysosomal le mafu a hepatobiliary.