4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Phello ea 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ke ho sebetsa e le substrate bakeng sa enzyme beta-glucosidase.Enzyme ena e qhaqha tlamahano ea glucosidic ea MUG, e leng se fellang ka ho lokolloa ha 4-Methylumbelliferon (4-MU) .Tšebeliso ea MUG e haholo-holo tšimong ea microbiology, ka ho khetheha bakeng sa ho lemoha le ho khetholla libaktheria tse hlahisang beta-glucosidase.MUG e atisa ho sebelisoa ha ho fumanoa Escherichia coli (E. coli) metsing le lijong.E. coli e na le enzyme ea beta-glucosidase, e ka etsang hydrolyze MUG le ho hlahisa lets'oao la fluorescent ka pel'a leseli la ultraviolet (UV). Thepa ea fluorescent ea 4-MU e lumella ho lemoha habonolo le ho lekanya.Ha substrate ea MUG e e-na le hydrolyzed, 4-MU e hlahisoang e hlahisa fluorescence e putsoa, ​​​​ho thusa ho tsebahatsa libaktheria tse nang le ts'ebetso ea beta-glucosidase.Mokhoa ona o atisa ho sebelisoa ho hlahloba tikoloho le tlhahlobo ea polokeho ea lijo, kaha o fana ka mokhoa o potlakileng le o bonolo oa ho lemoha tšilafalo ea baktheria. Ho phaella ts'ebetsong ea eona ho microbiology, MUG e ka boela ea sebelisoa ho ithuta mosebetsi le thibelo ea beta-glucosidase lipatlisiso tsa biochemistry le molecular biology.Fluorescence ea eona e thusa ho lekanya li-enzyme kinetics 'me e ka sebelisoa ho shebella li-inhibitors kapa li-activator tsa mosebetsi oa beta-glucosidase.Ka kakaretso, MUG ke motsoako o nang le mefuta e mengata o fumanang ts'ebeliso e atileng masimong a microbiology, biochemistry, le molecular biology bakeng sa ho lemoha. ts'ebetso ea beta-glucosidase le ho tsebahatsa libaktheria tse hlahisang enzyme ena.