ONPG CAS: 369-07-3 Theko ea Moetsi

Phello ea ONPG joalo ka substrate e lokela ho petsoha ke enzyme β-galactosidase, e leng se hlahisang ho lokolloa ha sehlahisoa se mosehla, o-nitrophenol.Phetoho ena ea 'mala e ka lekanyetsoa ka mokhoa oa spectrophotometrically, e lumellang hore ho be le palo ea mosebetsi oa β-galactosidase.Tšebeliso ea ONPG e haholo-holo tlhahlobong ea liphatsa tsa lefutso ho baeloji ea limolek'hule le lipatlisiso tsa microbiology.Hangata e sebelisoa ho lekanya tšebetso ea β-galactosidase e le motlalehi oa lithuto tsa liphatsa tsa lefutso, haholo-holo libaktheria tse kang E. coli.Mofuta oa lacZ, o kenyelletsang β-galactosidase, hangata o sebelisoa e le letšoao bakeng sa tlhahlobo ea polelo ea liphatsa tsa lefutso, kaha polelo ea eona e ka susumetsoa ke maemo a itseng kapa ea laoloa ke bahlahisi ba itseng. polelo ea liphatsa tsa lefutso ka ho lekanya tšebetso ea β-galactosidase.Teko ena e sebelisoa haholo lits'ebetsong tse fapaneng, joalo ka ho ithuta tšebetso ea batšehetsi, taolo ea liphatsa tsa lefutso, le tšebelisano ea protheine le protheine.Ho feta moo, e ka sebelisoa ho tseba kinetics ea enzyme le ho lekola litlamorao tsa liphetoho kapa kalafo ts'ebetsong ea enzyme.