HEPBS CAS:161308-36-7 Theko ea Moetsi

N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) (HEPBS) ke zwitterionic buffer eo hangata e sebelisoang liphuputsong tsa baeloji le biochemical.Phello ea eona e ka sehloohong ke ho thusa ho boloka pH e tsitsitseng litharollong, haholo-holo ka har'a physiological pH range (7.2-7.4).

Kopo e ka sehloohong eaHEPBS e ka seleng ea setso, moo e sebelisoang e le karolo ea litaba tsa setso ho boloka pH ea tharollo.E thusa ho fana ka tikoloho e tsitsitseng bakeng sa kholo ea lisele le ho thibela liphetoho leha e le life tse ka bang teng tsa pH tse ka bang kotsi ho lisele.

HEPBS e boetse e sebelisoa e le sesebelisoa se thibelang liphuputso tsa enzyme, kaha e ka tsitsisa pH nakong ea karabelo ea enzymatic.E sebelisoa hangata ho hloekisa protheine le litlhahlobo tsa enzymatic ho ntlafatsa ts'ebetso le botsitso ba li-enzyme.

Ho feta moo,HEPBS e sebelisoa ka mekhoa e fapaneng ea electrophoretic, joalo ka gel electrophoresis le capillary electrophoresis, ho boloka pH e lakatsehang le ho tsitsisa limolek'hule tse qosoang tse arohaneng.

Ntle le thepa ea eona ea buffer,HEPBS e ka boela ea sebetsa e le inhibitor e fokolang ea metalloprotein e itseng le li-enzyme, e leng se etsang hore e be molemo lits'ebetsong tse khethehileng.