FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, eo hape e tsejoang e le FMG, ke motsoako oa fluorescent o sebelisoang hangata e le substrate litekong tse fapaneng tsa biochemical le cell biology.E tsoa ho methyl-beta-D-galactopyranoside ka ho e kopanya le molek'hule ea fluorescein.FMG e sebelisoa haholo ho ithuta mosebetsi oa beta-galactosidase, e leng enzyme e etsang hore hydrolysis ea lactose e be galactose le glucose.Ka ho sebelisa FMG joalo ka substrate, bafuputsi ba ka lekola tšebetso ea enzymatic ea beta-galactosidase ka tekanyo ea tlhahiso ea fluorescence.Hydrolysis ea FMG ka beta-galactosidase e lebisa ho lokolloeng ha fluorescein, e leng se bakang keketseho ea lets'oao la fluorescent le ka lekanyetsoang.Motsoako ona o boetse o sebelisetsoa ho batlisisa ho lemoha le ho sebelisana ha lik'habohaedreite.FMG e ka sebelisoa e le lipatlisiso tsa limolek'hule ho ithuta kamano e tlamang ea li-lectins (liprotheine tse tlamang ka ho khetheha lik'habohaedreite) ho lik'habohaedreite tse nang le galactose.Ho tlamisoa ha li-complexes tsa FMG-lectin ho ka fumanoa le ho lekanyetsoa ho latela liphetoho tsa tlhahiso ea fluorescence.Ka kakaretso, FMG ke sesebelisoa se nang le mekhoa e mengata ea ho ithuta mosebetsi oa enzyme le ho lemoha lik'habohaedreite, ho fana ka mokhoa o bonolo le o hlokolosi oa ho lekanya fluorescence le ho hlahloba mekhoa ena ea likokoana-hloko.

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Fluorescein mono-beta-D-galactopyranoside (FMG) ke molek'hule e sebelisoang hangata lipatlisisong tsa baeloji e le karoloana ea ho bona boteng le tšebetso ea enzyme ea beta-galactosidase.FMG e tsoa ho lactose ea tsoekere 'me e kopantsoe le molek'hule ea fluorescein.

Phello e ka sehloohong ea FMG ke hore e na le hydrolyzed ka ho khetheha ke beta-galactosidase, enzyme e senyang lactose hore e be galactose le glucose.Enzymatic hydrolysis ena ea FMG e lebisa ho lokolloa ha fluorescein, e hlahisang lets'oao le matla la fluorescence.

Tšebeliso ea mantlha ea FMG ke ho lemoha le ho metha tšebetso ea beta-galactosidase ka lisampole tse fapaneng.Enzyme ena e fumaneha linthong tse ngata, ho kenyeletsoa libaktheria le lisele tsa mammalian, 'me mosebetsi oa eona e ka ba sesupo sa lits'ebetso tse fapaneng tsa lisele le mekhoa ea metabolic.

Ka ho sebelisa FMG joalo ka substrate, ts'ebetso ea beta-galactosidase e ka lekanyetsoa ka ho beha leihlo fluorescence e hlahisoang ke liberated fluorescein.Tekanyo ena e ka etsoa ka mekhoa e fapaneng ea liteko, ho kenyeletsoa li-in vitro assays le lithuto tsa litšoantšo tsa lisele.

Ho feta moo, FMG e ka sebelisoa e le sesebelisoa sa ho ithuta kabo le ho fumaneha ha beta-galactosidase ka har'a lisele.Ka ho sebelisa mekhoa ea microscopy ea fluorescent, bafuputsi ba ka bona ka mahlo a kelello fluorescence e hlahisoang ke FMG holim'a hydrolysis, e ba lumellang ho lekola tšebetso ea sebaka le ea nakoana ea beta-galactosidase.

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Tlhahisoleseling e 'Ngoe:

Sebopeho	C26H22O10
Tlhahlobo	99%
Ponahalo	Phofo e tšoeu
Nomoro ea CAS.	102286-67-9
Ho paka	Tse nyenyane le tse ngata
Shelf Life	lilemo tse 2
Polokelo	Boloka sebakeng se phodileng le se omileng
Setifikeiti	ISO.