FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside (FMG) ke molek'hule e sebelisoang haholo lipatlisisong tsa baeloji e le substrate ho lemoha boteng le ts'ebetso ea enzyme ea beta-galactosidase. FMG ke sehlahisoa sa lactose ea tsoekere 'me se kopantsoe le molek'hule ea fluorescein.

Phello e ka sehloohong ea FMG ke hore e haelloa ke hydrolyze ka ho khetheha ke beta-galactosidase, e leng enzyme e qhaqhang lactose hore e be galactose le glucose. Hydrolysis ena ea enzyme ea FMG e lebisa ho lokolloeng ha fluorescein, e ntšang lets'oao le matla la fluorescence.

Tšebeliso ea mantlha ea FMG ke ho lemoha le ho lekanya ts'ebetso ea beta-galactosidase lisampoleng tse fapaneng. Enzyme ena e fumanoa linthong tse ngata tse phelang, ho kenyeletsoa libaktheria le lisele tsa liphoofolo tse anyesang, 'me ts'ebetso ea eona e ka ba sesupo sa lits'ebetso tse fapaneng tsa sele le litsela tsa metabolism.

Ka ho sebelisa FMG e le substrate, ts'ebetso ea beta-galactosidase e ka lekanyetsoa ka ho beha leihlo khanya e ntšoang ke fluorescein e lokolotsoeng. Tekanyo ena e ka etsoa mefuteng e fapaneng ea liteko, ho kenyeletsoa liteko tsa in vitro le lithuto tsa ho nka litšoantšo tsa lisele tse phelang.

Ho feta moo, FMG e ka sebediswa e le sesebediswa sa ho ithuta kabo le sebaka sa beta-galactosidase ka hara disele. Ka ho sebedisa mekgwa ya microscopy ya fluorescent, bafuputsi ba ka bona lesedi le tswang ho FMG ha e hydrolysis, e leng se ba dumellang ho latela tshebetso ya sebaka le ya nako ya beta-galactosidase.