Phenylgalactoside, eo hape e tsejoang e le p-nitrophenyl β-D-galactopyranoside (pNPG), ke substrate ea maiketsetso e sebelisoang khafetsa litekong tsa biochemical le molecular biology.Hangata e sebelisoa ho lemoha le ho lekanya tšebetso ea enzyme ea β-galactosidase.
Ha phenylgalactoside e entsoe ka hydrolyzed ke β-galactosidase, e ntša p-nitrophenol, e leng motsoako oa 'mala o mosehla.Tokollo ea p-nitrophenol e ka lekanyetsoa ka bongata ho sebelisoa spectrophotometer, kaha monyetla oa p-nitrophenol o ka bonoa ka bolelele ba 405 nm.