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PNPG CAS: 3150-24-1 Theko ea Moetsi
PNPG, kapa p-nitrophenyl β-D-glucopyranoside, ke karoloana e nyenyane ea molek'hule e atisang ho sebelisoa litlhahlobong tsa lik'hemik'hale tsa biochemical ho lekanya tšebetso ea li-enzyme tsa glucosidase.Ha e na 'mala ebile ha e na fluorescent, empa ha hydrolysis e etsoa ke glucosidase, e fetoloa ho p-nitrophenol, e nang le' mala o mosehla 'me e bonahala habonolo spectrophotometrically.
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ONPG CAS: 369-07-3 Theko ea Moetsi
O-nitrophenyl-β-D-galactopyranoside (ONPG) ke substrate ea maiketsetso e sebelisoang litlhahlobong tsa biochemical le molecular biology ho lekanya tšebetso ea enzyme β-galactosidase.Hangata e sebelisoa litlhahlobong tsa ho lemoha liphatsa tsa lefutso tsamaisong ea baktheria, joalo ka Escherichia coli.ONPG ke motsoako o se nang 'mala o phunyeletsoeng ke β-galactosidase, e leng se hlahisang ho lokolloa ha motsoako o mosehla, o-nitrophenol.Mmala o mosehla o hlahisoang o ka lekanyetsoa spectrophotometrically, ho fana ka tekanyo e sa tobang ea ts'ebetso ea enzyme.Teko e sebelisang ONPG hangata e bitsoa tlhahlobo ea ONPG mme ke mokhoa o sebelisoang haholo lipatlisisong tsa baeloji ea limolek'hule ho lekola maemo a polelo a liphatsa tsa lefutso tse laoloang ke lac. operon liseleng tsa baktheria.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) ke sesupo sa redox se atisang ho sebelisoa litlhahlobong tsa baeloji le tsa biochemical.Ke phofo e mosehla e mosehla e fetohang boputsoa ha e fokotsehile, e etsa hore e be molemo bakeng sa ho lemoha boteng ba li-enzyme tse itseng le ts'ebetso ea metabolism.
NBT e sebetsana le li-electron carriers le li-enzyme tse kang dehydrogenases, tse amehang mekhoeng e sa tšoaneng ea lisele.Ha NBT e fokotsoa ke li-enzyme tsena, e etsa putsoa ea formazan precipitate, e lumellang ho lemoha pono kapa spectrophotometric.
Reagent ena e atisa ho sebelisoa litlhahlobong tse kang tlhahlobo ea phokotso ea NBT, moo e sebelisetsoang ho lekola tšebetso ea phefumoloho ea lisele tsa 'mele.E ka boela ea sebelisoa ho ithuta mesebetsi ea enzyme le litsela tsa metabolic lipatlisisong tse amanang le khatello ea oxidative, bokhoni ba lisele, le phapang ea lisele.
NBT e fumane lits'ebetso mafapheng a fapaneng, ho kenyeletsoa microbiology, immunology, le cell biology.E na le mekhoa e mengata, e tsitsitse, 'me e bonolo ho e sebelisa, e etsa hore e be khetho e tsebahalang bakeng sa liprothokholo tse ngata tsa liteko.
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Neocuproine CAS: 484-11-7 Theko ea Moetsi
Neocuproine ke chelating agent e sebelisoang hangata lits'ebetsong tse fapaneng, ho kenyeletsoa chemistry ea tlhahlobo le meriana.E na le kamano e matla bakeng sa li-ion tsa koporo 'me e etsa li-complexes tse tsitsitseng le tsona.Thepa ena e etsa hore neocuproine e be molemo bakeng sa ho bona le ho lekanya koporo ka tharollo kapa lisampole.Ho feta moo, neocuproine e ithutoe bakeng sa ts'ebeliso ea eona ea kalafo e ka bang teng, haholoholo kalafong ea mofetše le mafu a neurodegenerative.
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IPTG CAS: 367-93-1 Theko ea Moetsi
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ke analoge ea maiketsetso ea lactose e sebelisoang hangata liphuputsong tsa baeloji ea limolek'hule le lits'ebetsong tsa biotechnology.IPTG e sebelisoa haholo-holo ho susumetsa tlhahiso ea liphatsa tsa lefutso tsamaisong ea baktheria, moo e sebetsang e le sesosa sa molek'hule ho qala ho ngoloa ha liphatsa tsa lefutso.
Ha e kenyelelitsoe mocheng oa ho hōla, IPTG e nkoa ke libaktheria 'me e ka tlama protheine ea lac repressor, e thibela ho thibela mosebetsi oa lac operon.Lac operon ke sehlopha sa liphatsa tsa lefutso tse amehang ho metabolism ea lactose, 'me ha protheine e hatellang e tlosoa, liphatsa tsa lefutso li hlahisoa.
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HATU CAS:148893-10-1 Theko ea Moetsi
HATU (1- [bis(dimethylamino) methylene] -1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate) ke reagent e atisang ho sebelisoa ho kopanya peptide synthesis le organic chemistry.
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D-fucose CAS: 3615-37-0 Theko ea Moetsi
D-fucose ke monosaccharide, haholo-holo tsoekere e nang le carbon e tšeletseng, e leng ea sehlopha sa tsoekere e bonolo e bitsoang hexoses.Ke isomer ea tsoekere, e fapaneng ka tlhophiso ea sehlopha se le seng sa hydroxyl.
D-fucose ka tlhaho e fumaneha linthong tse fapaneng, ho kenyelletsa libaktheria, li-fungus, limela le liphoofolo.E bapala karolo ea bohlokoa lits'ebetsong tse 'maloa tsa tlhaho, joalo ka ho bonts'a lisele, ho khomarela lisele, le glycoprotein synthesis.Ke karolo ea glycolipids, glycoprotein, le proteoglycans, tse amehang puisanong ea sele le lisele le kananelo.
Ho batho, D-fucose e boetse e ameha ho biosynthesis ea likarolo tsa bohlokoa tsa glycan, tse kang li-antigen tsa Lewis le li-antigens tsa sehlopha sa mali, tse nang le liphello tsa ho lumellana le tšelo ea mali le ho ba le tšoaetso ea mafu.
D-fucose e ka fumanoa mehloling e fapaneng, ho kenyelletsa le limela tsa leoatleng, limela le ho belisoa ha likokoana-hloko.E sebelisoa lipatlisisong le lits'ebetsong tsa biomedical, hammoho le tlhahiso ea meriana e itseng le metsoako ea phekolo.
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DDT CAS: 3483-12-3 Theko ea Moetsi
DL-Dithiothreitol, eo hape e tsejoang e le DTT, ke ntho e fokotsang e sebelisoang haholo lipatlisisong tsa biochemical le molecular biology.Ke molek'hule e nyane e nang le sehlopha sa thiol (se nang le sebabole) pheletsong ka 'ngoe.
Hangata DTT e sebelisoa ho khaola maqhama a disulfide ka liprotheine, tse thusang ho li hlahisa kapa ho li hlahisa.Phokotso ena ea litlamo tsa disulfide e bohlokoa lits'ebetsong tse fapaneng tsa laboratori tse kang tlhoekiso ea protheine, gel electrophoresis, le lithuto tsa sebopeho sa protheine.DTT e ka boela ea sebelisoa ho sireletsa lihlopha tsa thiol le ho thibela oxidation nakong ea mekhoa ea liteko.
DTT ka tloaelo e eketsoa ho tharollo ea liteko maemong a manyane, mme ts'ebetso ea eona e ipapisitse le boteng ba oksijene.Ke habohlokoa ho sebetsana le DTT ka hloko kaha e na le maikutlo a moea, mocheso le mongobo, e leng se ka fokotsang katleho ea eona.
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D-(+)-Galactose CAS: 59-23-4 Theko ea Moetsi
D-(+) -Galactose ke tsoekere ea monosaccharide le karolo ea bohlokoa ea mekhoa e mengata ea likokoana-hloko.Ke tsoekere ea tlhaho e fumanehang lijong tse ngata, joalo ka litholoana, lihlahisoa tsa lebese le meroho.
Galactose e atisa ho sebelisoa 'meleng ka letoto la karabelo ea enzymatic.E bapala karolo ea bohlokoa puisanong ea lisele, tlhahiso ea matla, le biosynthesis ea limolek'hule tsa bohlokoa joalo ka glycolipids, glycoprotein le lactose.
Mabapi le lits'ebetso tsa eona, D-(+) -Galactose e sebelisoa hangata ho microbiology le biotechnology e le mohloli oa khabone mecheng ea litaba tsa setso bakeng sa kholo ea likokoana-hloko tse fapaneng.E boetse e sebelisoa ho hlahisa metsoako e fapaneng ea bioactive, meriana le lihlahisoa tsa lijo.Ho feta moo, hangata e sebelisoa e le moemeli oa tlhahlobo ea bongaka, haholo litekong tsa ho lekola tšebetso ea sebete le ho lemoha mathata a lefutso a amanang le metabolism ea galactose.
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beta-D-Galactose pentaacetate CAS: 4163-60-4
Beta-D-Galactose pentaacetate ke motsoako oa lik'hemik'hale o tsoang ho galactose, tsoekere ea monosaccharide.E thehoa ka acetylating sehlopha se seng le se seng sa hydroxyl ea molek'hule ea galactose e nang le lihlopha tse hlano tsa acetyl.
Motsoako ona o atisa ho sebelisoa e le moemeli oa tšireletso bakeng sa galactose liketsong tse sa tšoaneng tsa lik'hemik'hale le mekhoa ea maiketsetso.Mofuta oa pentaacetate o thusa ho tsitsisa galactose le ho thibela karabelo e sa batleheng kapa liphetoho nakong ea karabelo.
Ho feta moo, motsoako ona o ka sebelisoa e le selelekela sa ho qaptjoa ha lintho tse ling tse tsoang ho galactose.Lihlopha tsa acetyl li ka tlosoa ka mokhoa o ikhethileng ho fumana li-derivatives tse fapaneng tsa galactose ka lihlopha tse itseng tse sebetsang.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium letsoai CAS:129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium letsoai ke motsoako oa lik'hemik'hale o sebelisoang hangata liphuputsong tsa laboratori le tlhahlobo ea mafu.Hangata e bitsoa X-Gluc 'me e sebelisoa haholo e le setsi sa ho lemoha tšebetso ea enzyme ea beta-glucuronidase.
Ha beta-glucuronidase e le teng, e khaola tlamahano ea glucuronide ho X-Gluc, e leng se hlahisang ho lokolloa ha dae e putsoa e bitsoang 5-bromo-4-chloro-3-indolyl.Karabelo ena hangata e sebelisoa ho bona kapa ka spectrophotometric ho bona polelo ea enzyme ea beta-glucuronidase liseleng kapa liseleng.
Mofuta oa letsoai oa sodium oa X-Gluc o ntlafatsa ho qhibiliha ha oona ka har'a litharollo tse nang le metsi, ho nolofatsa tšebeliso ea ona litekong tsa laboratori.X-Gluc e sebelisoa haholo lipatlisisong tsa baeloji ea limolek'hule ho ithuta polelo ea liphatsa tsa lefutso, ts'ebetso ea batšehetsi, le liteko tsa liphatsa tsa lefutso tsa moqolotsi.E ka boela ea sebelisoa ho lemoha boteng ba likokoana-hloko tse hlahisang beta-glucuronidase, tse kang libaktheria tse itseng, lithutong tsa microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside ke karoloana ea chromogenic e sebelisoang litekong tsa enzymatic ho lemoha le ho lekanya tšebetso ea li-enzyme tse bitsoang beta-xylosidase.
