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N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid letsoai la sodium CAS:66992-27-6
N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid letsoai la sodium, le tsejoang hape e le HEPES sodium letsoai, ke motsoako oa lik'hemik'hale o atisang ho sebelisoa e le pH buffering agent lilaboratoring tsa baeloji le lik'hemik'hale.E thusa ho boloka pH e tsitsitseng, e etsa hore e be e loketseng bakeng sa lits'ebetso tse kang setso sa lisele, liteko tsa li-enzyme, lithuto tsa protheine, electrophoresis, le metsoako ea meriana.HEPES letsoai la sodium le netefatsa maemo a nepahetseng bakeng sa lits'ebetso tsa baeloji le ho ntlafatsa ho nepahala le ho ts'epahala ha liphetho tsa liteko.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide ke motsoako oa lik'hemik'hale o sebelisoang hangata litekong tsa biochemical le enzymatic.Ke substrate bakeng sa enzyme butyrylcholinesterase (BChE) 'me e sebelisetsoa ho lekanya mosebetsi oa eona.
Ha S-Butyrylthiocholine iodide e hydrolyzed ke BChE, e hlahisa thiocholine le butyric acid e le lihlahisoa.Ho lokolloa ha thiocholine ho ka lekanyetsoa ho sebelisoa spectrophotometric kapa fluorometric assay, ho lumella quantification ea mosebetsi oa BChE.
S-Butyrylthiocholine iodide hangata e sebelisoa litsing tsa kliniki le tsa lipatlisiso ho lekola ts'ebetso ea BChE ka lisampole tse kang plasma ea mali kapa lisele.E ka sebelisoa ho lekola ts'ebetso ea enzymatic ea BChE le karolo ea eona e ka bang teng lits'ebetsong tse fapaneng tsa bioloji, hammoho le tlhahlobong le tlhokomelong ea maemo a itseng a bongaka.
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ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) letsoai la diammonium) CAS:30931-67-0
Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), eo hangata e bitsoang ABTS, ke karoloana e sebelisoang hangata ea chromogenic litekong tsa biochemical, haholo lefapheng la enzymology.Ke motsoako oa maiketsetso o sebelisetsoang ho lekanya tšebetso ea li-enzyme tse fapaneng, ho kenyelletsa li-peroxidase le oxidase.
ABTS ha e na 'mala ka sebopeho sa eona sa oxidized empa e fetoha botala bo boputsoa ha e tšeloa ke enzyme ka pel'a hydrogen peroxide kapa molecular oxygen.Phetoho ena ea 'mala e bakoa ke ho thehoa ha cation e matla, e nkang leseli ka har'a mahlaseli a bonahalang.
Karabelo lipakeng tsa ABTS le enzyme e hlahisa sehlahisoa se mebala se ka lekantsoeng spectrophotometrically.Matla a 'mala a lekana ka kotloloho le ts'ebetso ea enzymatic, e lumellang bafuputsi ho lekola ka bongata enzyme kinetics, inhibition ea enzyme, kapa litšebelisano tsa enzyme-substrate.
ABTS e na le mefuta e mengata ea lits'ebetso makaleng a fapaneng, ho kenyelletsa tlhahlobo ea bongaka, lipatlisiso tsa meriana le mahlale a lijo.E na le kutloelo-bohloko haholo 'me e fana ka mefuta e mengata e fapaneng, e etsa hore e be khetho e tsebahalang bakeng sa liteko tse ngata tsa biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside ke motsoako oa sehlopha sa li-link tsa α-glycosidic.E tsoa ho maltose, e leng disaccharide e entsoeng ka likarolo tse peli tsa tsoekere.Motsoakong ona, sehlopha sa hydroxyl sa yuniti ea pele ea tsoekere se nkeloa sebaka ke karolo ea nitrophenyl.
Motsoako ona hangata o sebelisoa e le karoloana litlhahlobong tsa enzymatic ho ithuta tšebetso ea li-enzyme tse fapaneng, haholo tse amehang ho metabolism ea carbohydrate.Sehlopha sa nitrophenyl se lumella ho lemoha habonolo le ho lekanya likarolo tsa enzymatic ka ho lekanya ho monya kapa fluorescence ea sehlahisoa se petsotsoeng.
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PIPES CAS: 5625-37-6 Theko ea Moetsi
PIPES (piperazine-1,4-bisethanesulfonic acid) ke motsoako oa zwitterionic buffering o atisang ho sebelisoa lipatlisisong tsa baeloji le lik'hemik'hale.Ke sekoaelo se sebetsang sa pH se nang le bokhoni bo phahameng ba ho boloka maemo a tsitsitseng a pH ho tloha ho 6.1 ho isa ho 7.5.PIPES e na le tšitiso e fokolang ho li-biomolecules 'me e loketse litlhahlobo tse itšetlehileng ka mocheso.Hangata e sebelisoa mekhoeng ea gel electrophoresis le tlhahiso ea meriana e le moemeli oa botsitso.Ka kakaretso, PIPES ke motsoako o feto-fetohang le o sebelisoang haholo maemong a fapaneng a liteko.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3',5,5'-Tetramethylbenzidine, eo hape e tsejoang e le TMB, ke motsoako oa lik'hemik'hale o atisang ho sebelisoa e le chromogenic substrate ka li-enzyme-linked immunosorbent assays (ELISA) le litlhahlobo tse ling tsa biochemical.Hangata e sebelisoa ho lemoha le ho lekanya boteng ba li-enzyme tse kang horseradish peroxidase (HRP) ka lisampole tse sa tšoaneng tsa likokoana-hloko.TMB e fetoha 'mala ho tloha ho se nang 'mala ho ea ho o moputsoa ka boteng ba li-enzyme tsena.Ka mor'a moo, karabelo e ka emisoa ka ho eketsa asiti e fetolang 'mala o moputsoa ho' mala oa ho qetela o mosehla.Matla a 'mala o mosehla a lekana le palo ea enzyme e teng, e lumellang hore ho be le palo.
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APS-5 CAS: 193884-53-6 Theko ea Moetsi
(4-Chlorophenyl)thio-methanol 1-(dihydrogen phosphate) letsoai la disodium (1: 2) ke motsoako oa lik'hemik'hale o leng oa sehlopha sa lihlahisoa tsa acridine.E na le tsamaiso ea ring 10-methylacridine e nang le sehlopha sa thioether se kopantsoeng le boemo ba 4-chlorophenyl.Motsoako ona o boetse o na le sehlopha sa methanol le lihlopha tse peli tsa phosphate tse sa nkeng lehlakore ka li-ion tsa sodium.
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5-Bromo-4-chloro-3-indolyl phosphate disodium letsoai CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) ke motsoako oa lik'hemik'hale o sebelisoang hangata ho baeloji ea limolek'hule le lits'ebetsong tsa biochemistry.Ke karolo ea chromogenic ea li-enzyme tsa alkaline phosphatase.
Hangata BCIP e sebelisoa hammoho le nitroblue tetrazolium (NBT) e le karoloana ea ho lemoha tšebetso ea alkaline phosphatase.Ha BCIP e le dephosphorylated ke alkaline phosphatase, ho hlaha mefuta e putsoa, e lumellang pono ea boteng kapa tšebetso ea enzyme.
Motsoako ona o na le thuso ka ho khetheha lits'ebetsong tse kang immunohistochemistry, in situ hybridization, le li-enzyme-linked immunosorbent assays (ELISAs) ho lemoha boteng kapa sebakeng sa li-biomolecules tse itseng kapa nucleic acid.Pula e putsoa e entsoeng ke BCIP e fana ka letšoao le bonahalang le thusang ho khetholla le ho hlahloba limolek'hule tse shebiloeng lisampoleng tsa liteko.
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Letsoai la letsoai la sodium CAS: 139-41-3 Theko ea Moetsi
N,N-Bis(2-hydroxyethyl) glycine sodium letsoai ke motsoako oa lik'hemik'hale o sebelisoang e le ntho e thibelang lits'ebetso tse fapaneng tsa biochemical le biophysical.E thusa ho boloka boemo ba pH bo tsitsitseng maemong a liteko, ho etsa hore e be molemo lithutong tsa enzyme, lipatlisiso tsa protheine, setso sa lisele, le mekhoa ea ho thibela Bophirimela.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, eo hape e tsejoang e le 4-APhH, ke motsoako oa lik'hemik'hale o nang le foromo ea molek'hule C8H8N2O.Ke ea sehlopha sa metsoako ea hydrazide 'me e tsoa ho phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) ke mofuta o fetotsoeng oa amino acid cysteine.E fana ka mohloli oa cysteine 'me e ka fetoloa habonolo ho tripeptide glutathione, antioxidant e matla 'meleng.NAC e tsebahala ka thepa ea eona ea antioxidant le mucolytic, e e etsang hore e be molemo lits'ebetsong tse fapaneng tsa bophelo bo botle.
E le antioxidant, NAC e thusa ho sireletsa lisele ho tsoa tšenyong e bakoang ke li-radicals tsa mahala, mefuta ea oksijene e sebetsang le chefo.E boetse e ts'ehetsa glutathione synthesis, e bapalang karolo ea bohlokoa lits'ebetsong tsa 'mele tsa detoxification le ho boloka sesole sa' mele se phetseng hantle.
NAC e ithutoe ka melemo ea eona e ka bang teng bophelong ba phefumoloho, haholo-holo ho batho ba nang le maemo a kang bronchitis e sa foleng, COPD le cystic fibrosis.E atisa ho sebelisoa e le expectorant ho thusa ho tšesaane le ho lokolla mucus, ho etsa hore ho be bonolo ho hloekisa litsela tsa moea.
Ho feta moo, NAC e bontšitse tšepiso ea ho tšehetsa bophelo bo botle ba sebete ka ho thusa ho tlosoa ha lintho tse chefo, tse kang acetaminophen, e tloaelehileng ea ho kokobetsa bohloko.E ka boela ea e-ba le liphello tse sireletsang khahlanong le tšenyo ea sebete e bakoang ke ho noa joala.
Ntle le thepa ea eona ea ts'ehetso ea antioxidant le phefumoloho, NAC e 'nile ea hlahlojoa bakeng sa melemo ea eona e ka bang teng bophelong ba kelello.Liphuputso tse ling li fana ka maikutlo a hore e ka ba le phello e ntle mathateng a maikutlo, joalo ka khatello ea maikutlo le khatello ea maikutlo (OCD).
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Acetylthiocholine iodide CAS: 1866-15-5
Acetylthiocholine iodide ke motsoako oa lik'hemik'hale o atisang ho sebelisoa e le substrate litekong tsa enzyme ho lekanya mosebetsi oa enzyme acetylcholinesterase (AChE).AChE ke enzyme e hydrolyzes neurotransmitter acetylcholine, mohato oa bohlokoa oa ho felisa phetiso ea matšoao lipakeng tsa lisele tsa methapo.
Ha acetylthiocholine iodide e sebelisoa ke AChE, sehlopha sa acetyl se tlosoa, se fella ka ho thehoa ha li-ion tsa thiocholine le acetate.Thiocholine e ntan'o sebetsana le reagent e se nang 'mala e bitsoang DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) ho hlahisa motsoako oa 'mala o mosehla o bitsoang 5-thio-2-nitrobenzoate, o ka lekanngoang spectrophotometrically.Sekhahla sa nts'etsopele ea mebala se lekana ka kotloloho le mosebetsi oa AChE sampole.
