5,5-Dithiobis (2-nitrobenzoic acid) CAS: 69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), e tsejoang hape e le DTNB kapa Ellman's reagent, ke motsoako oa lik'hemik'hale o sebelisoang haholo lipatlisisong tsa biochemical le tsa meriana. Ke phofo e mosehla, e kristale e qhibilihang haholo metsing le li-solvents tsa organic.

DTNB e sebediswa haholoholo ho lekanya mahloriso a dihlopha tsa sulfhydryl (-SH) diprotheineng le dipeptide. Motswako ona o arabela le di-thiol, e leng se fellang ka ho thehwa ha disulfide e tswakilweng mme o lokolla 2-nitro-5-thiobenzoate, e nang le mmala o mosehla o ka lekanngwang ka spectrophotometric. Ka ho lekanya matla a mmala o mosehla, ho a kgoneha ho hakanya mahloriso a di-thiol sampole. Mokgwa ona o atisa ho sebediswa ho ithuta dikamano tsa protheine le protheine, ho mena ha protheine, le dikarabelo tsa enzymatic tse kenyeletsang dihlopha tsa thiol.

Ntle le ho lekanya thiol, DTNB e sebelisitsoe lits'ebetsong tse ling, tse kang ho lekola boemo ba redox ba lisele, ho ithuta botsitso ba metsoako ea tšepe-sulfure, le ho lekanya ts'ebetso ea li-enzyme tse fapaneng.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Thiol Quantification: DTNB e atisa ho sebelisoa ho lekola mahloriso a thiol disampoleng tsa protheine. Tlhahisoleseling ena e bohlokoa bakeng sa ho ithuta likamano tsa protheine le protheine, ho mena ha protheine, le liketso tsa enzymatic tse amang lihlopha tsa thiol. Karabelo ea colorimetric ea DTNB e lumella ho lekanya thiols ka litekanyo tsa spectrophotometric.

Tlhahlobo ea Redox State: DTNB e sebelisitsoe ho lekola boemo ba redox ka har'a lisele, lisele, kapa lisampole tsa baeloji. Ka ho lekanya liphetoho tsa mahloriso a thiol, bafuputsi ba ka fumana temohisiso mabapi le khatello ea oxidative, ts'ebetso ea antioxidant, le litsela tsa ho supa redox.

Liteko tsa Enzyme: Karabelo e ikhethang ea DTNB ka thiols e etsa hore e be sesebelisoa sa bohlokoa bakeng sa liteko tsa enzyme. Lienzyme tse ngata, tse kang glutathione-S-transferases, cystathionine-γ-lyase, le thioredoxin reductase, li itšetlehile ka thiol 'me li ka lekanngoa kapa tsa lekoa ho sebelisoa DTNB.

Tlhahlobo ea Botsitso ba Metsoako ea Tšepe le Sebabole: DTNB e sebelisetsoa ho lekola botsitso le karabelo ea metsoako ea tšepe le sebabole le liprotheine tsa tšepe. Motsoako o ka lemoha le ho lekanya boteng ba li-thiol tse amehang ho chelation ea li-ion tsa tšepe, o fana ka tlhaiso-leseling ea bohlokoa mabapi le likamano tsa tšepe le sebabole le k'hemistri ea khokahano.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C14H8N2O8S2
Teko	99%
Ponahalo	Sete e mosehla
Nomoro ea CAS	69-78-3
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.