4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Phello: ONPG ke karoloana e sebelisoang ka ho khetheha ho bona boteng le tšebetso ea enzyme ea β-galactosidase.Ha enzyme ea β-galactosidase e le teng ebile e sebetsa, e arola ONPG hore e be lihlahisoa tse peli: o-nitrophenol le derivative ea galactose.Ho lokolloa ha o-nitrophenol ho fella ka phetoho ea 'mala o mosehla, e ka lekanngoang ho sebelisoa spectrophotometer.

Kopo: ONPG e na le lits'ebetso tse 'maloa ho baeloji ea limolek'hule le lipatlisiso tsa biochemistry:

Boikemisetso ba ts'ebetso ea β-galactosidase: ONPG e sebelisoa hangata ho lekanya le ho lekanya tšebetso ea enzyme ea β-galactosidase.Sekhahla sa sebopeho sa o-nitrophenol, se lekanang ka kotloloho le ts'ebetso ea enzyme, se ka lekanyetsoa spectrophotometrically.

Polelo ea liphatsa tsa lefutso le taolo: ONPG hangata e sebelisoa litekong tse amanang le polelo ea lefutso le lithuto tsa taolo.E sebelisoa khafetsa litekong tsa protheine ea fusion, joalo ka sistimi e sebelisoang hangata ea lacZ fusion, ho ithuta polelo ea liphatsa tsa lefutso tlas'a taolo ea batšehetsi ba itseng.Ts'ebetso ea beta-galactosidase e lekantsoeng ho sebelisoa ONPG e fana ka leseli mabapi le boemo ba polelo ea lefutso.

Ho hlahloba ts'ebetso ea β-galactosidase: ONPG e ka sebelisoa e le mokhoa oa ho hlahloba colorimetric ho theknoloji ea recombinant DNA ho tseba boteng le ts'ebetso ea mofuta oa LacZ, o kenyelletsang β-galactosidase.Mokhoa ona oa ho hlahloba o thusa ho khetholla li-clones tse nang le mofuta oa thahasello.

Lithuto tsa kinetics tsa enzyme: ONPG e boetse e na le thuso ho ithuteng kinetics ea enzyme ea β-galactosidase.Ka ho lekanya sekhahla sa karabelo ea enzyme-substrate libakeng tse fapaneng tsa substrate, hoa khonahala ho tseba li-parameter tsa kinetic tse kang Michaelis-Menten constants (Km) le maximum reaction rates (Vmax).