4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Phello: ONPG ke substrate e sebediswang ka ho kgetheha ho lemoha boteng le tshebetso ya enzyme ya β-galactosidase. Ha enzyme ya β-galactosidase e le teng mme e sebetsa, e arola ONPG hore e be dihlahiswa tse pedi: o-nitrophenol le derivative ya galactose. Ho lokollwa ha o-nitrophenol ho fella ka phetoho ya mmala o mosehla, e ka lekanngwang ho sebediswa spectrophotometer.

Kopo: ONPG e na le lits'ebetso tse 'maloa lipatlisisong tsa baeloji ea limolek'hule le biochemistry:

Ho fumana ts'ebetso ea β-galactosidase: ONPG e sebelisoa hangata ho lekanya le ho lekanya ts'ebetso ea enzyme ea β-galactosidase. Sekhahla sa ho thehoa ha o-nitrophenol, se lekanang ka ho toba le ts'ebetso ea enzyme, se ka lekanyetsoa ka spectrophotometric.

Tlhaloso le taolo ea liphatsa tsa lefutso: ONPG hangata e sebelisoa litekong tse amanang le tlhahlobo ea liphatsa tsa lefutso le lithuto tsa taolo. E sebelisoa khafetsa litekong tsa protheine ea ho kopanya, joalo ka sistimi ea ho kopanya ea lacZ e sebelisoang hangata, ho ithuta tlhahiso ea liphatsa tsa lefutso tlas'a taolo ea batšehetsi ba itseng. Mosebetsi oa beta-galactosidase o lekantsoeng ho sebelisoa ONPG o fana ka temohisiso mabapi le boemo ba tlhahiso ea liphatsa tsa lefutso.

Tlhahlobo bakeng sa ts'ebetso ea β-galactosidase: ONPG e ka sebelisoa e le mokhoa oa tlhahlobo ea mebala-bala theknolojing ea DNA e kopantsoeng ho khetholla boteng le ts'ebetso ea gene ea LacZ, e kenyang β-galactosidase. Mokhoa ona oa tlhahlobo o thusa ho khetholla li-clone tse nang le gene e khahlisang.

Lithuto tsa kinetics tsa enzyme: ONPG e boetse e na le thuso ho ithuteng kinetics ea enzyme ea β-galactosidase. Ka ho lekanya sekhahla sa karabelo ea enzyme-substrate maemong a fapaneng a substrate, hoa khoneha ho fumana liparamente tsa kinetic tse kang li-constant tsa Michaelis-Menten (Km) le sekhahla se phahameng sa karabelo (Vmax).