3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ho sibolloa ha ts'ebetso ea beta-galactosidase: ONPG hangata e sebelisoa ho tseba boteng le ts'ebetso ea beta-galactosidase lisampoleng tse fapaneng tsa tlhaho, joalo ka litloaelo tsa baktheria kapa li-lysate tsa lisele.Tlhahiso ea o-nitrophenol, e nang le 'mala o mosehla, e ka lekanyetsoa habonolo ho sebelisa spectrophotometer.

Lithuto tsa polelo ea liphatsa tsa lefutso: ONPG e sebelisoa hangata lipatlisisong tsa baeloji ea limolek'hule ho ithuta polelo ea lefutso.Ka ho kopanya motšehetsi oa mofuta oa thahasello le mofuta oa encoding beta-galactosidase, bafuputsi ba ka lekanya tšebetso ea mothetsi enoa ka ho eketsa ONPG le ho lekanya tlhahiso ea o-nitrophenol.Mokhoa ona, o tsejoang e le tlhahlobo ea moqolotsi oa litaba oa beta-galactosidase, o fana ka leseli mabapi le ts'ebetso ea mongolo oa lefutso.

Boitsebiso ba baktheria: Libaktheria tse ling li hlahisa beta-galactosidase, ha tse ling li sa etse joalo.ONPG e ka sebelisoa hammoho le liteko tse ling tsa biochemical ho tseba mefuta ea baktheria e ipapisitseng le bokhoni ba eona ba ho hlahisa ONPG.Mokhoa ona o sebelisoa hangata lilaboratoring tsa tlhahlobo ea bongaka le li-microbiology.

Ho hlahloba li-enzyme inhibitors kapa activators: ONPG e ka sebelisoa ho lekola metsoako e tsamaisang tšebetso ea beta-galactosidase.Ka ho lekanya tšebetso ea enzyme moo ho nang le metsoako e fapaneng, bafuputsi ba ka tseba li-inhibitors kapa li-activator tse ka batlisisoang ho ea pele bakeng sa matla a bona a kalafo.