3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) ke sesepa se sebediswang haholo ho biochemistry le baeloji ya dimolek'hule. Ke sesepa sa zwitterionic, ho bolelang hore se na le sehlopha se nang le tjhaje e ntle le e mpe.

CHAPS e tsebahala ka bokgoni ba yona ba ho qhibilihisa le ho tsitsisa diprotheine tsa lera, e leng se etsang hore e be molemo bakeng sa ditshebediso tse fapaneng tse kang ho ntsha protheine, ho e hlwekisa le ho e hlalosa. E sitisa dikamano tsa lipid le protheine, e dumella diprotheine tsa lera ho ntshwa boemong ba tsona ba tlhaho.

Ho fapana le disepa tse ding, CHAPS e bobebe haholo mme ha e fokotse boholo ba protheine, e leng se etsang hore e be kgetho e ntle bakeng sa ho boloka sebopeho le tshebetso ya protheine nakong ya diteko. E ka boela ya thusa ho thibela ho kopana ha protheine.

CHAPS e atisa ho sebelisoa mekhoeng e kang SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, le Western blotting. E boetse e sebelisoa khafetsa lithutong tse kenyeletsang li-enzyme tse tlamiloeng ka lera, phetisetso ea matšoao, le likamano tsa protheine le lipid.

Odara kapa Kopa Khoteshene

Qaqiso ea Sehlahisoa

Li-tag tsa Sehlahisoa

Tšebeliso le Phello

Ho Ntša Protheine: CHAPS e atisa ho sebelisoa ho ntša liprotheine tsa lera ho tsoa lisampoleng tsa baeloji. E thusa ho qhibilihisa liprotheine tsena le ho boloka sebopeho sa tsona sa tlhaho.

Ho Hloekisa Protheine: CHAPS e sebelisoa mekhoeng e fapaneng ea ho hloekisa protheine, joalo ka affinity chromatography. E ka eketsoa ho li-buffer tsa ho hloekisa ho qhibilihisa le ho tsitsisa liprotheine tsa lera nakong ea ts'ebetso ea ho hloekisa.

Tlhaloso ea Liprotheine: CHAPS e atisa ho sebelisoa lithutong tse kenyeletsang tlhaloso ea liprotheine tsa lera. E thusa ho boloka sebopeho le ts'ebetso ea protheine nakong ea mekhoa ea liteko joalo ka liteko tsa ts'ebetso ea enzyme, likamano tsa protheine le protheine, le litlhahlobo tsa spectroscopic.

Lithuto tsa Protheine ea Lera: Liprotheine tsa Lera li phetha karolo ea bohlokoa lits'ebetsong tse ngata tsa sele. CHAPS e sebelisoa hangata lipatlisisong tse amanang le phetisetso ea matšoao, ts'ebetso ea kanale ea ion, likamano tsa protheine le lipid, le kristale ea protheine ea lera.

Electrophoresis: CHAPS e sebelisoa mekhoeng e kang SDS-PAGE le isoelectric e shebaneng le ho qhibilihisa liprotheine tsa lera le ho nolofatsa karohano le tlhahlobo ea tsona.

Ho paka Sehlahisoa:

Tlhahisoleseling e 'Ngoe:

Sebopeho	C32H58N2O7S
Teko	99%
Ponahalo	Phofo e tšoeu ea kristale
Nomoro ea CAS	75621-03-3
Ho paka	E nyane le e ngata
Bophelo ba Shelofo	lilemo tse 2
Polokelo	Boloka sebakeng se pholileng le se omileng
Setifikeiti	ISO.