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3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3--[(3-cholamidopropyl)dimethylammonio] -1-propanesulfonate) ke sesepa se sebelisoang hangata ho baeloji le baeloji ea limolek'hule.Ke sesepa sa zwitterionic, ho bolelang hore se na le sehlopha se nang le qoso e ntle le e mpe.

CHAPS e tsebahala ka bokhoni ba eona ba ho hlapa le ho tsitsisa liprotheine tsa membrane, ho e etsa hore e be molemo bakeng sa lits'ebetso tse fapaneng joalo ka ho ntšoa ha protheine, tlhoekiso le sebopeho.E sitisa tšebelisano ea lipid-protein, e lumellang liprotheine tsa membrane hore li ntšoe sebakeng sa habo tsona.

Ho fapana le lisebelisoa tse ling tse hloekisang, CHAPS e batla e le bobebe 'me ha e fane ka liprotheine tse ngata, e leng se etsang hore e be khetho e ntle ea ho boloka sebopeho sa protheine le ts'ebetso nakong ea liteko.E ka boela ea thusa ho thibela ho kopana ha protheine.

CHAPS e sebelisoa hangata mekhoeng e kang SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, le Western blotting.E boetse e sebelisoa khafetsa lithutong tse amang li-enzyme tse tlanngoeng ke membrane, phetisetso ea matšoao le litšebelisano tsa protheine-lipid.


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Protein Extraction: CHAPS e atisa ho sebelisoa ho ntša liprotheine tsa lera ho lisampole tsa baeloji.E thusa ho kopanya liprotheine tsena le ho boloka sebopeho sa tsona sa tlhaho.

Tlhoekiso ea Liprotheine: CHAPS e sebelisoa mekhoeng e fapaneng ea tlhoekiso ea protheine, joalo ka affinity chromatography.E ka eketsoa ho li-buffers tsa tlhoekiso ho qhibilihisa le ho tsitsisa liprotheine tsa membrane nakong ea ts'ebetso ea tlhoekiso.

Sebopeho sa Protein: CHAPS e sebelisoa hangata lithutong tse kenyelletsang sebopeho sa liprotheine tsa lera.E thusa ho boloka sebopeho sa protheine le ts'ebetso nakong ea lits'ebetso tsa liteko joalo ka liteko tsa ts'ebetso ea enzyme, tšebelisano ea protheine le protheine, le tlhahlobo ea spectroscopic.

Lithuto tsa Protein ea Membrane: Liprotheine tsa Membrane li bapala karolo ea bohlokoa lits'ebetsong tse ngata tsa cellular.CHAPS e atisa ho sebelisoa liphuputsong tse amanang le phetisetso ea matšoao, ts'ebetso ea mocha oa ion, litšebelisano tsa protheine-lipid, le membrane ea protheine ea crystallization.

Electrophoresis: CHAPS e sebelisoa mekhoeng e kang SDS-PAGE le isoelectric e shebaneng le ho qhibilihisa liprotheine tsa lera le ho thusa karohano le tlhahlobo ea tsona.

 

Ho paka Sehlahisoa:

6892-68-8-3

Tlhahisoleseling e 'Ngoe:

Sebopeho C32H58N2O7S
Tlhahlobo 99%
Ponahalo Phofo e tšoeu ea kristale
Nomoro ea CAS. 75621-03-3
Ho paka Tse nyenyane le tse ngata
Shelf Life lilemo tse 2
Polokelo Boloka sebakeng se phodileng le se omileng
Setifikeiti ISO.

 


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